Article
Biochemistry & Molecular Biology
Susanne Soelch, Nathalie Beaufort, Daniela Loessner, Matthias Kotzsch, Ute Reuning, Thomas Luther, Thomas Kirchner, Viktor Magdolen
Summary: The study shows that Rab31 is a potent regulator of TGF-β1 and other components of TGF-β signaling pathway in breast cancer cells, impacting genes involved in EMT processes.
MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Araceli Lopez-Tejada, Carmen Grinan-Lison, Adrian Gonzalez-Gonzalez, Francisca E. Cara, Rafael J. Luque, Carmen Rosa-Garrido, Jose L. Blaya-Canovas, Alba Navarro-Ocon, Maria Valenzuela-Torres, Marisa Parra-Lopez, Jesus Calahorra, Isabel Blancas, Juan A. Marchal, Sergio Granados-Principal
Summary: In triple-negative breast cancer (TNBC), the phosphorylation and localization of NDRG1 play an important role in its pleiotropic effects. TGFβ1 has been found to regulate different aspects of TNBC progression, including migration, invasion, epithelial-mesenchymal transition, and cancer stem cell maintenance, through the downstream activation of NDRG1 and NF-κB. The combination of TGFβ and GSK3β inhibitors can impair cancer stem cells in TNBC. The assessment of both total NDRG1 and p-NDRG1 (Thr346) positivity and subcellular localization is crucial for evaluating patient prognosis and stratification.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Bernhard J. Jank, Julia Schnoell, Katharina Kladnik, Carmen Sparr, Markus Haas, Elisabeth Gurnhofer, Alexander L. Lein, Markus Brunner, Lukas Kenner, Lorenz Kadletz-Wanke, Gregor Heiduschka
Summary: This study explored the role of the TGF-β pathway in HNSCC and evaluated the antineoplastic and radiosensitizing effects of the TGFBR1 inhibitor, vactosertib, in vitro.
Article
Cell Biology
Zhenxia Zhang, Min Lin, Junli Wang, Fenglian Yang, Peikui Yang, Yaqun Liu, Zikai Chen, Yuzhong Zheng
Summary: Calycosin inhibited proliferation, migration, and invasion of breast cancer cells by downregulating BATF and TGF beta 1 signaling, thus suppressing epithelial-mesenchymal transition (EMT) and progression. These findings suggest calycosin as a promising therapeutic option for breast cancer patients.
Article
Biochemistry & Molecular Biology
Bashdar Mahmud Hussen, Hazha Jamal Hidayat, Snur Rasool Abdullah, Sayran Mohamadtahr, Mohammad Fatih Rasul, Majid Samsami, Mohammad Taheri
Summary: This review focuses on the functional properties of lncRNAs and the role of dysregulated TGF-β signaling in BC. Both lncRNAs and the TGF-β pathway play important roles in BC diagnosis, prognosis, and drug resistance.
Article
Genetics & Heredity
Xiu-Xia Zhang, Jun-Hua Luo, Li-Qiang Wu
Summary: This study revealed that high expression of fibronectin 1 (FN1) is correlated with poor clinical outcomes and serves as an independent prognostic factor in breast cancer (BRCA). FN1 is also associated with tumor-infiltrating immune cells and immune checkpoint inhibitors, potentially aiding in the treatment of BRCA patients. The results highlight FN1 as a novel prognostic biomarker and therapeutic target for BRCA.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Yinpei Huang, Zhili Liu, Xin Wang, Yaoxu Li, Lian Liu, Bing Li
Summary: Intermittent hypoxia (IH) is the primary pathological manifestation of obstructive sleep apnea (OSA) and the main cause of OSA-induced cognitive impairment. Transforming growth factor-β3 (TGF-β3) plays a crucial role in resisting hypoxic brain injury, but its role in IH-induced neuronal injury is still unclear. This study aimed to clarify the mechanism of TGF-β3 protecting IH-exposed neurons by regulating oxidative stress and secondary apoptosis.
NEUROCHEMICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Lakshmi Vineela Nalla, Piyush Gondaliya, Kiran Kalia, Amit Khairnar
Summary: This study revealed that miR-128-3p inhibits SP1, thereby limiting TGF-beta 1 induced EMT in breast cancer cell lines. A series of experiments were conducted to explore the mechanism of action of miR-128-3p mimics.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Yingying Yu, Weiwei Wang, Wenying Lu, Wei Chen, Anquan Shang
Summary: Accumulating evidence suggests that INHBA plays a crucial role in promoting migration and invasion of breast cancer cells by inducing EMT through activating TGF-beta-regulated genes, which potentially make it a diagnostic biomarker and therapeutic target for breast cancer treatment.
Article
Engineering, Biomedical
Sejal Patwardhan, Pratiksha Mahadik, Omshree Shetty, Shamik Sen
Summary: Exosomes play a significant role in breast cancer invasiveness triggered by ECM stiffness. High ECM stiffness promotes exosome secretion in a YAP/TAZ pathway-dependent manner, leading to changes in cell morphology, adhesion, and protrusion dynamics that enhance cell motility and invasion. Exosomes from stiff ECM cultures exhibit enrichment of cell adhesion and migration proteins, with thrombospondin-1 identified as a prospective regulator of stiffness-dependent cancer invasion mediated by exosomal communication.
Article
Endocrinology & Metabolism
Jie Xu, Na Xiao, Hailang He, Ailing Cao, Xianmei Zhou
Summary: The study aimed to investigate the action and mechanism of Asperuloside on non-small cell lung cancer (NSCLC). The results showed that Asperuloside significantly suppressed the proliferation, invasion, and migration abilities of NSCLC cells, promoted apoptosis, and reduced tumor size and weight in mice. Moreover, Asperuloside inhibited the TGF-β1/Smad2 pathway, resulting in down-regulation of N-Cadherin and Vimentin protein levels and up-regulation of E-Cadherin protein level.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Biochemistry & Molecular Biology
Duaa Alkaabi, Kholoud Arafat, Shahrazad Sulaiman, Aya Mudhafar Al-Azawi, Samir Attoub
Summary: Triple-negative breast cancer (TNBC) is a highly aggressive type of breast cancer with poor prognosis. This study investigated the role of programmed death-ligand 1 (PD-L1) in TNBC MDA-MB-231 cells independent of its binding to PD-1 receptors on T cells. The study found that knockout of PD-L1 inhibited cell proliferation, colony formation, migration, and invasion in vitro, as well as tumor growth in a chick embryo model in vivo. PD-L1 knockout also affected the expression of several downstream proteins involved in tumor progression. These findings suggest that targeting PD-L1 could be a potential therapeutic strategy for TNBC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Bai Cui, Bin He, Yanping Huang, Cenxin Wang, Huandong Luo, Jinxin Lu, Keyu Su, Xiaoyu Zhang, Yuanyuan Luo, Zhuoran Zhao, Yuqing Yang, Yunkun Zhang, Fan An, Hong Wang, Eric W. -F. Lam, Keith W. Kelley, Ling Wang, Quentin Liu, Fei Peng
Summary: This study reveals the critical role of proline synthetase PYCR1 in maintaining breast cancer stemness and tumor growth. Proline synthesized by PYCR1 activates the cGMP-PKG signaling pathway to enhance cancer stem-like traits. Psychological stress can induce cancer stem-like phenotypes and tumorigenesis mediated by cGMP-PKG signaling.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Shoucheng Huang, Ping Huang, Huazhang Wu, Song Wang, Guodong Liu
Summary: The study identified that LINC02381 is overexpressed in breast cancer and plays crucial roles in tumor progression by promoting proliferation, migration, and invasion of cancer cells. LINC02381 functions as a competing endogenous RNA to increase FN1 by sponging miR-1271-5p, ultimately activating the PI3K/AKT pathway. Rescuing FN1 upregulation or PI3K/AKT activation could counteract the inhibitory effects of LINC02381 knockdown on malignant phenotypes of breast cancer cells.
MOLECULAR CARCINOGENESIS
(2022)
Article
Medicine, Research & Experimental
Siwei Pan, Jiaming Zhu, Pengfei Liu, Qiaochu Wei, Siyu Zhang, Wen An, Yuxin Tong, Zhenguo Cheng, Funan Liu
Summary: The study suggests that FN1 3'-UTR has stronger prognostic implications than FN1 protein in gastric cancer patients and can be a better therapeutic target for constructing targeted drugs.
Article
Gastroenterology & Hepatology
Camille Peneau, Sandrine Imbeaud, Tiziana La Bella, Theo Z. Hirsch, Stefano Caruso, Julien Calderaro, Valerie Paradis, Jean-Frederic Blanc, Eric Letouze, Jean-Charles Nault, Giuliana Amaddeo, Jessica Zucman-Rossi
Summary: This study aimed to characterize HBV integrations in liver tissues of HCC patients and revealed new mechanisms of HBV-associated oncogenesis. HBV integrations have direct oncogenic consequences, posing a challenge for the monitoring of HBV-infected patients.
Article
Oncology
Yann-Alexandre Vano, Reza Elaidi, Mostefa Bennamoun, Christine Chevreau, Delphine Borchiellini, Diane Pannier, Denis Maillet, Marine Gross-Goupil, Christophe Tournigand, Brigitte Laguerre, Philippe Barthelemy, Elodie Coquan, Gwenaelle Gravis, Nadine Houede, Mathilde Cancel, Olivier Huillard, Philippe Beuzeboc, Laure Fournier, Arnaud Mejean, Xavier Cathelineau, Nicolas Doumerc, Philippe Paparel, Jean-Christophe Bernhard, Alexandre de la Taille, Karim Bensalah, Thibault Tricard, Thibaut Waeckel, Geraldine Pignot, Elena Braychenko, Stefano Caruso, Cheng-Ming Sun, Virginie Verkarre, Guillaume Lacroix, Marco Moreira, Maxime Meylan, Antoine Bougouin, Letuan Phan, Christelle Thibault-Carpentier, Jessica Zucman-Rossi, Wolf Herman Fridman, Catherine Sautes-Fridman, Stephane Oudard
Summary: This study demonstrated the feasibility and positive impact of prospective patient selection based on tumor molecular phenotype to choose the most effective treatment between nivolumab with or without ipilimumab and a VEGFR-TKI in the first-line treatment of metastatic clear-cell renal cell carcinoma.
Article
Medical Laboratory Technology
Francesca Di Gaudio, Giuseppina Brunacci, Flavia Contino, Alessia Gallo, Fabio Centineo
Summary: This study developed and conducted an External Quality Assessment (EQA) panel of assay to evaluate the quality of real-time reverse transcription polymerase chain reaction (PCR) used by 62 Sicilian laboratories. The results showed that 75% of the laboratories tested all samples correctly, while the remaining 25% interpreted the EQA exercise samples incorrectly, not meeting the required standards.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Computer Science, Information Systems
Samir Hassoun, Chiara Bruckmann, Stefano Ciardullo, Gianluca Perseghin, Francesca Di Gaudio, Francesco Broccolo
Summary: This study developed a machine learning algorithm to diagnose liver fibrosis in the general US population. By utilizing data processing and feature selection, a model capable of identifying liver fibrosis was obtained and its feasibility was confirmed in a subset of participants.
INTERNATIONAL JOURNAL OF MEDICAL INFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Ignazio Restivo, Alessandro Attanzio, Ilenia Concetta Giardina, Francesca Di Gaudio, Luisa Tesoriere, Mario Allegra
Summary: Research shows that exposure to cigarette smoke can induce apoptosis in red blood cells, involving the initiation of DISC formation by p38 MAPK and activation of caspase-8/caspase-3 through ceramide generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Isabella Polidori, Laura Marullo, Cristiano Ialongo, Flaminia Tomassetti, Roberto Colombo, Francesca di Gaudio, Graziella Calugi, Giulia Marrone, Annalisa Noce, Sergio Bernardini, Francesco Broccolo, Massimo Pieri
Summary: This study examines the dysbiosis status in the Italian population, specifically focusing on patients with type 2 diabetes (T2D) and metabolic disorders. The results show a bias towards Proteobacteria in T2D patients, potentially influenced by their dietary habits. It was also observed that T2D females have altered gut permeability, which may increase the risk of vaginal infections.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Review
Biochemistry & Molecular Biology
Giusi Alberti, Eleonora Russo, Simona Corrao, Rita Anzalone, Peter Kruzliak, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: Extracellular vesicles (EVs) are one of the main mechanisms of communication between cells and the surrounding tissue or at distance. Recent studies have revealed the molecular composition and clinical indications of EVs derived from adult mesenchymal stromal cells (MSCs). These EVs maintain some of the properties of their parent cells and have important implications for cell-free therapies.
Article
Chemistry, Multidisciplinary
Francesca Di Gaudio, Diana Amorello, Marzia Ferrara, Santino Orecchio, Silvia Orecchio
Summary: The aim of this study was to develop an analytical method to quantitatively determine the concentration of 18 elements in tattoo inks using Inductively Coupled Plasma Mass Spectrometry. The results showed that the total concentrations of metals in the 16 analyzed tattoo ink samples ranged from 0.060 to 16.9 g kg(-1). Zinc was the most abundant metal, with concentrations ranging from 3.4 to 13882 mg kg(-1). The concentrations of zinc in three samples exceeded the legal limit (2000 μg g(-1)), and the concentration of chromium in all cases was higher than allowed (0.5 μg g(-1)). Four of the analyzed samples should not be used by tattoo artists according to the limit values.
Review
Cell Biology
Eleonora Russo, Simona Corrao, Francesca Di Gaudio, Giusi Alberti, Martin Caprnda, Peter Kubatka, Peter Kruzliak, Vitale Miceli, Pier Giulio Conaldi, Cesario Venturina Borlongan, Giampiero La Rocca
Summary: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in over 650 million cases and 6.6 million deaths worldwide. The respiratory system is primarily affected, with typical symptoms including fever, cough, sore throat, acute respiratory distress syndrome (ARDS), and fatigue. The abnormal inflammatory response known as the cytokine storm can lead to multiorgan disease and death. While vaccines have been developed to prevent infection, treating severe cases remains challenging. Researchers are exploring new strategies, such as cell therapy using perinatal tissues enriched with mesenchymal stromal cells (MSCs), which have anti-inflammatory properties, modulate immune responses, and promote tissue repair. This review focuses on umbilical-cord-derived MSCs (UC-MSCs) and their potential as a therapeutic strategy, based on in vitro and in vivo studies, as well as recent clinical trials.
Article
Chemistry, Analytical
Francesca Di Gaudio, Salvatore Barreca, Santino Orecchio
Summary: In this study, an analytical method based on reversed-phase ion-pair chromatography coupled with mass spectrometry was optimized for the quantification of ultra-trace levels of DADMAC in drinking water. The method was validated and showed good accuracy, precision, linearity range, and limits of quantification and detection. DADMAC was detected in all analyzed samples, with concentrations ranging from 1.2 to 3.8 μg/L and a mean value of 1.7 μg/L. This is the first study on DADMAC concentration in water samples collected in Italy, and it is of significant interest for future comparison of DADMAC monitoring data in water. Moreover, this study highlights one of the few cases where DADMAC has been found in drinking water.
Article
Nutrition & Dietetics
Valentina Guarnotta, Roberta Amodei, Francesca Di Gaudio, Carla Giordano
Summary: This study confirms the efficacy of a ketogenic diet as an adjunctive therapy for patients with Cushing's disease, showing significant improvements in metabolic disorders. Furthermore, it suggests that a nutritional approach can be combined with conventional treatment for Cushing's disease to improve metabolic and cardiovascular comorbidities.
Review
Cell Biology
Eleonora Russo, Giusi Alberti, Simona Corrao, Cesar V. Borlongan, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: The potential of perinatal cells and their secreted EVs in regenerative medicine is well established. They exhibit therapeutic effects such as tissue repair, immune system modulation, and other functions. However, challenges remain in characterizing and utilizing these cells and EVs.
Article
Obstetrics & Gynecology
Giuseppe Giordano, Giuseppe Gullo, Marco Scaglione, Giovanni Buzzaccarini, Gaspare Cucinella, Domenico Gullo, Daniela Segreto, Vito Chiantera, Antonio Simone Lagana, Francesca Di Gaudio
Summary: Gender medicine is an innovative medical approach that studies the influence of sex and gender on biological variables. This study investigated the correlation between heavy metal exposure and neurodevelopment pathologies in newborns, according to their sex. Results showed no significant differences related to foetal sex in terms of congenital malformations or other variables, but these findings provide a valuable foundation for future research in the field of gender medicine.
MENOPAUSE REVIEW-PRZEGLAD MENOPAUZALNY
(2023)
Article
Biochemistry & Molecular Biology
Giovanni L. Scaglione, Sandro Pignata, Angela Pettinato, Carmela Paolillo, Daniela Califano, Giuseppa Scandurra, Valentina Lombardo, Francesca Di Gaudio, Basilio Pecorino, Liliana Mereu, Paolo Scollo, Ettore D. Capoluongo
Summary: This study compares two different methods of shallow whole-genome sequencing with a reference assay in HGSOC samples. The results show good correlation between the two methods and their correlation with patient's response to treatment. The study also finds prognostic value in the HRD score.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Giuseppa Augello, Mariamena Arbitrio, Lydia Giannitrapani, Francesca Scionti, Domenico Ciliberto, Nicoletta Staropoli, Giuseppe Agapito, Pierfrancesco Tassone, Pierosandro Tagliaferri, Maurizio Soresi, Aurelio Seidita, Marco Affronti, Gaetano Bertino, Maurizio Russello, Francesca Di Gaudio, Rosaria Ciriminna, Francesca Spinnato, Francesco Verderame, Melchiorre Cervello
JOURNAL OF HEPATOLOGY
(2023)
