Article
Biochemistry & Molecular Biology
Tao Wu, Xiaotian Liu, Ting Wang, Li Tian, Hao Qiu, Feng Ge, Jing Zhu, Liang Shi, Ailiang Jiang, Hanshou Yu, Ang Ren
Summary: This study found that the HO/CO system can respond to heat stress and regulate Ganoderma lucidum's ganoderic acid biosynthesis and cell wall integrity by regulating the Slt-MAPK phosphorylation level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Khosrow Kashfi, Kush K. Patel
Summary: This article reviews the history of carbon monoxide, highlighting its toxic effects as well as its roles as an endogenously produced molecule and therapeutic agent. The research into its physiological functions and therapeutic applications are often overshadowed by its reputation as a lethal substance.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yixiao Luo, Rafi Ullah, Jinfeng Wang, Yuru Du, Shihao Huang, Li Meng, Yuan Gao, Miao Gong, Ewa Galaj, Xi Yin, Haishui Shi
Summary: The study demonstrated that carbon monoxide (CO) has significant antidepressant and anxiolytic effects, providing protection through activation of HO-1 and exogenous CO administration. This research revealed a novel function of the HO-1/CO system, offering a promising therapeutic target for the treatment of depression and anxiety.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xingrong Lai, Yanhua Sun, Xuedi Zhang, Dan Wang, Jialing Wang, Haihua Wang, Yao Zhao, Xinling Liu, Xin Xu, Haoran Song, Wenjia Ping, Yanli Sun, Zhenbo Hu
Summary: Honokiol is an effective anti-leukemia agent that reduces the viability of AML cells and induces cell cycle arrest. It triggers a noncanonical ferroptosis pathway by upregulating intracellular lipid peroxide and HMOX1 levels.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lucia Longhitano, Giuseppe Broggi, Sebastiano Giallongo, Maria Failla, Lidia Puzzo, Teresio Avitabile, Daniele Tibullo, Alfio Distefano, Valeria Pittala, Michele Reibaldi, Guido Nicola Zanghi, Antonio Longo, Andrea Russo, Rosario Caltabiano, Giovanni Li Volti, Nicolo Musso
Summary: The study revealed the important role of HO-1 in regulating uveal melanoma progression, with an increase of HO-1 promoting cellular proliferation and wound healing ability of tumor cells, suggesting HO-1 protein expression may serve as a potential prognostic and therapeutic factor in UM patients.
Review
Biochemistry & Molecular Biology
Yoon Kyung Choi, Young-Myeong Kim
Summary: Heme oxygenase and its metabolites have dual roles in neurovascular diseases, potentially causing cytotoxic ferroptosis via iron accumulation, but also exhibiting regenerative potential and neurovascular protective effects through CO-mediated signaling pathway, antioxidant properties of bilirubin, and iron-mediated ferritin synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Kinga Krukowska, Marcin Magierowski
Summary: Gastrointestinal tract cancers present a challenge for researchers in terms of finding effective molecular agents for targeted therapies. Ongoing clinical trials offer new perspectives, but long-term treatments remain unsuccessful. Novel pharmacological and molecular approaches are being studied for the prevention and treatment of GI cancers. Additionally, pharmacological tools that release carbon monoxide have shown therapeutic properties in treating GI pathologies.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Chemistry, Inorganic & Nuclear
Ana C. Pena, Ana Pamplona
Summary: Transition metals, especially iron, play important roles in the chemistry of life. Hemoproteins, including hemoglobin, are common targets for binding with biologically active gases such as NO and CO. Heme oxygenases, particularly HO-1, are crucial enzymes involved in heme metabolism and have documented antioxidant and anti-inflammatory properties. However, their role in infection, such as malaria, can be dual, either limiting pathogen burden or promoting pathogen growth. CO-releasing molecules have been considered as a potential alternative to CO gas inhalation for therapeutic purposes.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Health Care Sciences & Services
Jun Fang, Rayhanul Islam, Shanghui Gao, Cheng Zhang, Ryotaro Kunisaki, Shogo Sakaguchi, Naoya Honda, Jian-Rong Zhou, Kazumi Yokomizo
Summary: Heme oxygenase (HO-1) is crucial for cellular protection against stresses and diseases, but its overexpression in tumors is also commonly observed. Maintaining a balance of HO-1 levels in both host and tumor cells is essential for cancer occurrence, progression, and prognosis, with high HO-1 levels in the host being beneficial for cancer prevention and suppressing HO-1 in tumor cells possibly becoming a therapeutic strategy.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cell Biology
Ramachandran Srinivasan, Hyo-Shim Han, Parthiban Subramanian, Anbazhagan Mageswari, Seong-Hoon Kim, Srikanth Tirumani, Vaibhav Kumar Maurya, Gothandam Kodiveri Muthukaliannan, Mohandass Ramya
Summary: The phenomenon of heat stress leading to ferroptosis-like cell death has recently been observed in bacteria as well as plant cells. However, the evidence of ferroptosis in microalgae remains unknown. This study investigated the induction of reactive oxygen species (ROS) and iron-dependent ferroptotic cell death in Chlamydomonas reinhardtii, comparing it with RSL3-induced ferroptosis. The results showed that both heat shock and RSL3 treatment triggered ferroptotic cell death with several characteristic biomarkers, indicating that ferroptotic cell death affects iron homeostasis and lipid peroxidation metabolism in Chlamydomonas.
Article
Biochemistry & Molecular Biology
Libor Vitek
Summary: The liver has evolved defense systems to protect against harmful stimuli, including antioxidant substances and enzymes, anti-inflammatory tools, enzymatic biotransformation systems, and metabolic pathways. Recent research indicates the significant role of the heme catabolic pathway in regulating the pathogenesis of liver and other diseases.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Chemistry, Multidisciplinary
Xiaodi Rong, Yiyuan Peng, Caiyun Liu, Mingzhu Li, Jiafan Shi, Miaohui Yu, Shuaikang Ba, Wenlong Sheng, Baocun Zhu
Summary: In this study, the issues of sensitivity, selectivity, and water solubility of CO fluorescent probes were investigated. A probe named HS-CO was designed to greatly increase the rigid plane of the probe through intramolecular cyclization, resulting in high sensitivity for CO detection. The probe was successfully used to detect CO in HeLa cells and zebrafish, demonstrating its great potential as a tool for functional analysis of CO in organisms.
NEW JOURNAL OF CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Tamas Gall, David Petho, Annamaria Nagy, Gyorgy Balla, Jozsef Balla
Summary: The study shows that oxidized hemoglobins, globin-derived peptides, and heme trigger diverse biological responses, including toll-like receptor 4 activation with inflammatory response, reprogramming of cellular metabolism, differentiation, stress, and even death. The damage caused by oxidized hemoglobin and heme to cells and tissues is related to the pathological consequences of hemorrhage and hemolysis in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Stefan W. Ryter
Summary: Heme molecule is essential for oxygen transport, cellular metabolic enzyme activities, and can promote human diseases if dysfunction occurs. It has pro-oxidant properties, inducing cytotoxicity and injury, while also modulating inflammatory and immune system functions. Therapeutic strategies targeting the heme/HO-1 pathway show potential in inflammatory conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Slawomir Nowak, Przemyslaw Gilun, Katarzyna Koziol, Maria Romerowicz-Misielak, Magdalena Koziorowska-Gilun, Barbara Wasowska
Summary: Exogenous CO regulates mRNA transcription and protein expression of HO-1, HO-2, and PCNA, similar to a light cue.
Article
Chemistry, Inorganic & Nuclear
Kaplan Kirakci, Michaela Kubanova, Tomas Pribyl, Michaela Rumlova, Jaroslav Zelenka, Tomas Ruml, Kamil Lang
Summary: Singlet oxygen photosensitizers that target cell membranes have been developed for enhanced photodynamic therapy. A singlet oxygen photosensitizing complex, made of a molybdenum-iodine nanocluster stabilized by triazolate apical ligands, was prepared using copper-free click chemistry. This complex targeted cell membranes and exhibited a significant phototoxic effect against HeLa cells. The nature of the apical ligands plays a dominant role in the biological properties of the nanocluster. The complex also showed antibacterial effects on pathogenic strains.
INORGANIC CHEMISTRY
(2022)
Article
Biochemical Research Methods
Marketa Kulisova, Olga Matatkova, Tomas Branyik, Jaroslav Zelenka, Lucie Drabova, Irena Jarosova Kolouchova
Summary: Microscopic filamentous fungi are adaptable microorganisms that can form biofilms on various surfaces. This study aimed to find a reliable method for measuring the biofilm growth of selected fungi strains. Multiple methods were tested, and crystal violet staining was found to be suitable for determining total biofilm biomass, while the MTT assay was suitable for determining its metabolic activity. Fluorescent stains successfully dyed the hyphae of microscopic fungi.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Gastroenterology & Hepatology
Katerina Zizalova, Barbora Novakova, Marek Vecka, Jaromir Petrtyl, Vera Lanska, Kveta Pelinkova, Vaclav Smid, Radan Bruha, Libor Vitek, Martin Lenicek
Summary: The study suggests that taurochenodeoxycholic acid (TCDCA) may be a promising noninvasive marker of clinically significant portal hypertension. Its predictive potential may be further enhanced when combined with the AST/ALT ratio and spleen diameter.
LIVER INTERNATIONAL
(2023)
Article
Chemistry, Organic
Marek Martinek, Lucie Ludvikova, Maria Srankova, Rafael Navratil, Lucie Muchova, Jiri Huzlik, Libor Vitek, Petr Klan, Peter Sebej
Summary: Fluorescein, eosin Y, and rose bengal, commonly used dyes in clinical medicine, have been found to release carbon monoxide (CO) and low-mass secondary photoproducts when extensively irradiated with visible light in aqueous solutions. This photochemistry should be taken into consideration in their applications and they could also be used as visible-light activatable CO-releasing molecules with biological implications.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Carmen C. C. Mayorga-Martinez, Marketa Castoralova, Jaroslav Zelenka, Tomas Ruml, Martin Pumera
Summary: In this study, a method based on magnetic microrobots was developed for rapid isolation and identification of Staphylococcus aureus bacteria in milk. The method does not produce harmful compounds for the milk microbiota and can be efficiently applied in food production.
Article
Chemistry, Multidisciplinary
Carmen C. Mayorga-Martinez, Jaroslav Zelenka, Karel Klima, Michaela Kubanova, Tomas Ruml, Martin Pumera
Summary: Modern micro/nanorobots have versatile capabilities for biomedical and environmental tasks. Magnetic microrobots are particularly promising for biomedical applications, as they can be fully controlled by a rotating magnetic field without the use of toxic fuels. These microrobots can also form swarms to perform tasks at a larger scale.
ADVANCED MATERIALS
(2023)
Article
Endocrinology & Metabolism
Ondrej Uher, Katerina Hadrava Vanova, Radka Lencova, Andrea Frejlachova, Herui Wang, Zhengping Zhuang, Jan Zenka, Karel Pacak
Summary: Cancer immunotherapy has achieved significant clinical progress recently. However, there is a lack of preclinical studies focused on age-dependent effects during cancer immunotherapy. This study compares the efficacy of a previously developed intratumoral immunotherapy in young and aged mice with pheochromocytoma. The results show that the immunotherapy is effective in both age groups, indicating its potential as a therapeutic intervention for tumors in both young and aged hosts.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Regis Guegan, Xiaoxue Cheng, Xiang Huang, Zuzana Nemeckova, Michaela Kubanova, Jaroslav Zelenka, Tomas Ruml, Fabien Grasset, Yoshiyuki Sugahara, Kamil Lang, Kaplan Kirakci
Summary: The photoinactivation of pathogenic bacteria using materials generating reactive oxygen species upon exposure to visible light can overcome the challenge of multidrug-resistant microbial pathogens. A nanocomposite material consisting of graphene oxide sheets adorned with nanoaggregates of octahedral molybdenum cluster complexes has been developed. The electrostatic interaction and close cohesion between the components in aqueous medium contributed to the potent antibacterial effect against Staphylococcus aureus upon illumination with blue light. The photoinduced electron transfer from the nanoaggregates to the graphene oxide sheets triggers the generation of reactive oxygen species, making graphene oxide and light-harvesting octahedral molybdenum cluster complexes promising for effective antibacterial strategies.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Alena Jiraskova, Jan Skrha, Libor Vitek
Summary: This study assessed serum bilirubin concentrations and microsatellite variations in UGT1A1 and HMOX1 genes in patients with type 2 diabetes mellitus (T2DM). Results showed that T2DM patients had lower serum bilirubin concentrations compared to controls, and the prevalence of phenotypic Gilbert syndrome and (TA)(7/7) UGT1A1 genotype was lower in male T2DM patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Inorganic & Nuclear
Regis Guegan, Xiaoxue Cheng, Xiang Huang, Zuzana Nemeckova, Michaela Kubanova, Jaroslav Zelenka, Tomas Ruml, Fabien Grasset, Yoshiyuki Sugahara, Kamil Lang, Kaplan Kirakci
Summary: The emergence of multidrug-resistant microbial pathogens poses a significant threat to effective antibiotic therapy. Photoinactivation using materials generating reactive oxygen species upon exposure to visible light can overcome this challenge by targeting vital components of living cells, reducing the likelihood of resistance development.
INORGANIC CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Suman Ghosal, Katerina Hadrava Vanova, Ondrej Uher, Shaoli Das, Mayank Patel, Leah Meuter, Thanh-Truc Huynh, Abhishek Jha, Sara Talvacchio, Marianne Knue, Tamara Prodanov, Martha A. Zeiger, Naris Nilubol, David Taieb, Joakim Crona, Uma T. Shankavaram, Karel Pacak
Summary: In this study, we analyzed transcriptomic data from NENs to identify immune cell infiltration signatures, particularly in PCPG. We validated the immune signature of PCPG using an independent cohort. We identified five dominant immune clusters in PCPG, associated with different immune cell types. Furthermore, we developed a prognostic immune score model that could predict non-aggressive and non-metastatic diseases.
Review
Sport Sciences
Kyle D. Flack, Libor Vitek, Christopher S. Fry, David E. Stec, Terry D. Hinds Jr
Summary: Exercise performance is influenced by various factors, including muscular strength, cardiovascular capacity, liver health, and metabolic flexibility. Recent studies have shown that exercise training can increase plasma levels of bilirubin, which has generally been considered a marker of liver dysfunction, and elite athletes may have higher levels. It is speculated that this adaptation could be due to the antioxidant properties of bilirubin in response to reactive oxygen species produced during exercise. Current research has redefined bilirubin as a metabolic hormone that interacts with nuclear receptors, potentially contributing to weight reduction. Additionally, bilirubin has been linked to reduced adiposity and improved cardiovascular function. While no studies have directly examined the impact of elevated bilirubin levels on athletic performance, it is an area worthy of exploration in future research.
FRONTIERS IN SPORTS AND ACTIVE LIVING
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Barbora Novakova, Katerina Zizalova, Vaclav Smid, Karel Dvorak, Ales Kubena, Libor Vitek, Martin Lenicek, Radan Bruha
JOURNAL OF HEPATOLOGY
(2023)