Article
Cell Biology
Xudong Duan, Xi Chen, Kangji Wang, Li Chen, Oliver Glomb, Nils Johnsson, Lin Feng, Xiao-Qiu Zhou, Erfei Bi
Summary: The study reveals that yeast Ecm25 plays a crucial role in connecting the polarization signal with the endocytic machinery to enable adaptive morphogenesis under stress conditions. Deletion of ECM25 impairs cell elongation under hydroxyurea-induced stress without affecting overall mother-bud polarity.
Review
Cell Biology
Marieke M. Glazenburg, Liedewij Laan
Summary: Cell polarization is an important phenomenon in the evolution of cellular life, driven by self-organization and leading to the establishment of preferential axis. The evolution of polarization can be studied using Saccharomyces cerevisiae and its fungal relatives as model species, providing insights on the relationship between self-organization and evolutionary change at the microscopic scale.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biology
Mirai Tanigawa, Katsuyoshi Yamamoto, Satoru Nagatoishi, Koji Nagata, Daisuke Noshiro, Nobuo N. Noda, Kouhei Tsumoto, Tatsuya Maeda
Summary: The study reveals that Pib2 acts as both a glutamine sensor and a direct TORC1 activator in budding yeast, providing a new model for the metabolic control of cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Yun Quan, Stephen M. Hinshaw, Pang-Che Wang, Stephen C. Harrison, Huilin Zhou
Summary: Chromosome segregation during cell division is controlled and adapted by kinetochores, which connect spindle microtubules to chromosomal centromeres. A mechanism that regulates kinetochore sumoylation has been identified, with a conserved surface of the Ctf3/CENP-I kinetochore protein providing a binding site for the SUMO chain-removing enzyme Ulp2. Disabling Ulp2 recruitment to kinetochores leads to defective chromosome segregation due to elevated sumoylation levels.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Nicholas J. McGlincy, Zuriah A. Meacham, Kendra K. Reynaud, Ryan Muller, Rachel Baum, Nicholas T. Ingolia
Summary: This study introduces a comprehensive yeast CRISPRi library based on empirical design rules, which has been validated to provide comprehensive genome coverage through competitive growth experiments. The fitness defects of essential genes caused by different guides targeting them were used to refine yeast CRISPRi design rules, and guide abundance measurements in pooled screens were improved by linking guides with random nucleotide barcodes and carrying out linear amplification.
Article
Biochemical Research Methods
Daniel Williamson, William Tasker-Brown, James A. H. Murray, Angharad R. Jones, Leah R. Band
Summary: This article investigates the mechanisms of cell-size control in plant cells and establishes a mathematical model to simulate the key interactions in the cell cycle. The study finds that both size-independent synthesis and equal inheritance of cell-cycle proteins contribute to cell-size control, but combining these mechanisms at the two key transitions in the cell cycle is essential in maintaining cell-size homeostasis.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Cell Biology
Mohammad Alfatah, Liang Cui, Corinna Jie Hui Goh, Trishia Yi Ning Cheng, Yizhong Zhang, Arshia Naaz, Jin Huei Wong, Jacqueline Lewis, Wei Jie Poh, Prakash Arumugam
Summary: This study demonstrates that differential glucose metabolism activates TORC1 through three distinct pathways in yeast. The first pathway requires conversion of glucose to fructose 1,6-bisphosphate, the second pathway involves conversion of glucose to glucose 6-phosphate and mitochondrial function, and the third pathway requires complete glycolysis and vacuolar ATPase reassembly.
Review
Biochemistry & Molecular Biology
Magdalena Foltman, Alberto Sanchez-Diaz
Summary: This article focuses on the significance of the highly conserved TOR signaling pathway, particularly the connection between TORC1 complex in budding yeast and the cell cycle, and the importance of this connection for cell size and chromosome stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Douglas R. Kellogg, Petra Anne Levin
Summary: Pioneering work discovered that bacterial cell size is proportional to growth rate set by nutrient availability, known as the growth law. Recent studies have shown that the relationship between growth rate, nutrients, and cell size is more complex than originally thought.
TRENDS IN CELL BIOLOGY
(2022)
Article
Cell Biology
Wangkai Xie, Zheng Han, Ziyi Zuo, Dong Xin, Hua Chen, Juanjuan Huang, Siyu Zhu, Han Lou, Zhiqiang Yu, Chenbin Chen, Sian Chen, Yuanbo Hu, Jingjing Huang, Fabiao Zhang, Zhonglin Ni, Xian Shen, Xiangyang Xue, Kezhi Lin
Summary: This study analyzed the relationship between differential expression of cytoskeleton genes and prognosis in gastric cancer (GC). ASAP1 was found to play a key role in cytoskeletal remodeling and prognosis in GC patients, and its overexpression can be used as a predictor of poor prognosis.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Pranas Grigaitis, Bas Teusink
Summary: Using computational modeling, it is suggested that yeast maintains an excess of glycolytic enzymes at low specific growth rates, which helps in adapting to sugar fluctuations in the environment.
Article
Biochemistry & Molecular Biology
Arun T. John Peter, Sabine N. S. van Schie, Ngaam J. Cheung, Agnes H. Michel, Matthias Peter, Benoit Kornmann
Summary: The membrane lipid composition of eukaryotic cells varies among different organelles. This study focuses on simplifying and reorganizing yeast phospholipid synthesis by redirecting synthesis reactions to specific subcellular locations. Through genetic screening, genes involved in lipid homeostasis, metabolism, and transport were identified, with a particular focus on the requirement for Csf1 in interorganelle lipid transport and homeostatic adaptation.
Review
Mathematics, Applied
Yue Liu, Jun Xie, Hay-Oak Park, Wing-Cheong Lo
Summary: The budding yeast Saccharomyces cerevisiae is a powerful model system for studying cell polarity establishment. Signaling molecules, initially distributed in the cytoplasm, are recruited to the cell membrane in response to spatial cues or spontaneously. Mathematical models have shed light on the complex regulation of polarization and can be used for future applications in understanding cell polarization and morphogenesis.
COMMUNICATIONS ON APPLIED MATHEMATICS AND COMPUTATION
(2023)
Review
Biochemistry & Molecular Biology
David J. J. Krause
Summary: Humans rely on budding yeasts' ability to grow without oxygen in industrial fermentations. Understanding the evolution and genetic basis of this adaptation could expand the use of useful species and provide insights into yeast ecology. However, there is still much to learn about the breadth of species and genes involved in anaerobic growth.
Article
Multidisciplinary Sciences
Debraj Ghose, Katherine Jacobs, Samuel Ramirez, Timothy Elston, Daniel Lew
Summary: This study investigated how small eukaryotic cells interpret chemical gradients, focusing on Saccharomyces cerevisiae cells. Experimental and computational modeling revealed that the movement of polarity sites is biased by local pheromone gradients, leading to effective gradient tracking. This mechanism may be relevant for cells navigating complex chemical landscapes to find targets.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Mid Eum Lee, Wing-Cheong Lo, Kristi E. Miller, Ching-Shan Chou, Hay-Oak Park
JOURNAL OF CELL SCIENCE
(2015)
Article
Biochemistry & Molecular Biology
Mandy Hiu Yi Lam, Jamie Snider, Monique Rehal, Victoria Wong, Farzaneh Aboualizadeh, Luka Drecun, Olivia Wong, Bella Jubran, Meirui Li, Mehrab Ali, Matthew Jessulat, Viktor Deineko, Rachel Miller, Mid Eum Lee, Hay-Oak Park, Alan Davidson, Mohan Babu, Igor Stagljar
JOURNAL OF MOLECULAR BIOLOGY
(2015)
Review
Biochemistry & Molecular Biology
Kristi E. Miller, Yeonsoo Kim, Won-Ki Huh, Hay-Oak Park
JOURNAL OF MOLECULAR BIOLOGY
(2015)
Article
Cell Biology
Kristi E. Miller, Wing-Cheong Lo, Mid Eum Lee, Pil Jung Kang, Hay-Oak Park
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Biology
Wing-Cheong Lo, Hay-Oak Park, Ching-Shan Chou
BULLETIN OF MATHEMATICAL BIOLOGY
(2014)
Article
Cell Biology
Pil Jung Kang, Jennifer K. Hood-DeGrenier, Hay-Oak Park
JOURNAL OF CELL SCIENCE
(2013)
Article
Cell Biology
Pil Jung Kang, Kristi E. Miller, Julia Guegueniat, Laure Beven, Hay-Oak Park
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Biochemistry & Molecular Biology
Jamie Snider, Asad Hanif, Mid Eum Lee, Ke Jin, Analyn R. Yu, Chris Graham, Matthew Chuk, Dunja Damjanovic, Marta Wierzbicka, Priscilla Tang, Dina Balderes, Victoria Wong, Matthew Jessulat, Katelyn D. Darowski, Bryan-Joseph San Luis, Igor Shevelev, Stephen L. Sturley, Charles Boone, Jack F. Greenblatt, Zhaolei Zhang, Christian M. Paumi, Mohan Babu, Hay-Oak Park, Susan Michaelis, Igor Stagljar
NATURE CHEMICAL BIOLOGY
(2013)
Article
Multidisciplinary Sciences
Wing-Cheong Lo, Mid Eum Lee, Monisha Narayan, Ching-Shan Chou, Hay-Oak Park
Article
Genetics & Heredity
Komudi Singh, Mid Eum Lee, Maryam Entezari, Chan-Hun Jung, Yeonsoo Kim, Youngmin Park, Jack D. Fioretti, Won-Ki Huh, Hay-Oak Park, Pil Jung Kang
G3-GENES GENOMES GENETICS
(2019)
Article
Cell Biology
Kristi E. Miller, Wing-Cheong Lo, Ching-Shan Chou, Hay-Oak Park
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Cell Biology
Pil Jung Kang, Rachel Mullner, Haoyu Li, Derek Hansford, Han-Wei Shen, Hay-Oak Park
Summary: Cdc42, a conserved Rho GTPase, plays a central role in polarity establishment in yeast and animals. The active Cdc42 level is sporadically elevated in wild type during repeated cell divisions but rarely in the longlived bud8 deletion cells. Genetic analyses and live-cell imaging suggest that Rga1 and Bud8 oppositely impact life span likely by modulating active Cdc42 levels. Our findings implicate that the interplay among these positive and negative polarity factors limits the life span of budding yeast.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Review
Cell Biology
Kristi E. Miller, Pil Jung Kang, Hay-Oak Park
Review
Cell Biology
Didac Carmona-Gutierrez, Maria Anna Bauer, Andreas Zimmermann, Andres Aguilera, Nicanor Austriaco, Kathryn Ayscough, Rena Balzan, Shoshana Bar-Nun, Antonio Barrientos, Peter Belenky, Marc Blondel, Ralf J. Braun, Michael Breitenbach, William C. Burhans, Sabrina Buttner, Duccio Cavalieri, Michael Chang, Katrina F. Cooper, Manuela Corte-Real, Vitor Costa, Christophe Cullin, Ian Dawes, Jorn Dengjel, Martin B. Dickman, Tobias Eisenberg, Birthe Fahrenkrog, Nicolas Fasel, Kai-Uwe Frohlich, Ali Gargouri, Sergio Giannattasio, Paola Goffrini, Campbell W. Gourlay, Chris M. Grant, Michael T. Greenwood, Nicoletta Guaragnella, Thomas Heger, Juergen Heinisch, Eva Herker, Johannes M. Herrmann, Sebastian Hofer, Antonio Jimenez-Ruiz, Helmut Jungwirth, Katharina Kainz, Dimitrios P. Kontoyiannis, Paula Ludovico, Stephen Manon, Enzo Martegani, Cristina Mazzoni, Lynn A. Megeney, Chris Meisinger, Jens Nielsen, Thomas Nystrom, Heinz D. Osiewacz, Tiago F. Outeiro, Hay-Oak Park, Tobias Pendl, Dina Petranovic, Stephane Picot, Peter Polcic, Ted Powers, Mark Ramsdale, Mark Rinnerthaler, Patrick Rockenfeller, Christoph Ruckenstuhl, Raffael Schaffrath, Maria Segovia, Fedor F. Severin, Amir Sharon, Stephan J. Sigrist, Cornelia Sommer-Ruck, Maria Joao Sousa, Johan M. Thevelein, Karin Thevissen, Vladimir Titorenko, Michel B. Toledano, Mick Tuite, F. -Nora Voegtle, Benedikt Westermann, Joris Winderickx, Silke Wissing, Stefan Woelfl, Zhaojie J. Zhang, Richard Y. Zhao, Bing Zhou, Lorenzo Galluzzi, Guido Kroemer, Frank Madeo