Review
Cell Biology
Virginia Andrade, Arnaud Echard
Summary: This paper reviews the mechanical context of cytokinetic abscission and summarizes the mechanisms of intercellular bridge tension release and abscission trigger. Additionally, it discusses whether traction-induced mechanical cell rupture could be an alternative mechanism of abscission and suggests future research directions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Bhavna Rani, Deepesh K. K. Gupta, Staffan Johansson, Siamak A. A. Kamranvar
Summary: Recent research indicates that integrin-mediated adhesion plays a role in regulating cell division at two crucial steps. This review examines the involvement of integrin signals in different stages of the cell cycle under normal conditions and the safety mechanisms that counteract the generation of aneuploid cells in cases of defective integrin signals.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Federico Gulluni, Lorenzo Prever, Huayi Li, Petra Krafcikova, Ilaria Corrado, Wen-Ting Lo, Jean Piero Margaria, Anlu Chen, Maria Chiara De Santis, Sophie J. Cnudde, Joseph Fogerty, Alex Yuan, Alberto Massarotti, Nasrin Torabi Sarijalo, Oscar Vadas, Roger L. Williams, Marcus Thelen, David R. Powell, Markus Schueler, Michael S. Wiesener, Tamas Balla, Hagit N. Baris, Dov Tiosano, Brian M. McDermott, Brian D. Perkins, Alessandra Ghigo, Miriam Martini, Volker Haucke, Evzen Boura, Giorgio Roberto Merlo, David A. Buchner, Emilio Hirsch
Summary: The cytokinetic process in the lens, which depends on PI3K-C2alpha, PI(3,4)P-2, and VPS36, is essential for preventing premature senescence and cataract. Loss of these components impairs cytokinesis and leads to cell damage, triggering senescence in the lens of fish, mice, and humans.
Article
Multidisciplinary Sciences
Julia Carrillo-Garcia, Victor Herrera-Fernandez, Selma A. Serra, Fanny Rubio-Moscardo, Marina Vogel-Gonzalez, Pablo Donate-Macian, Covadonga F. Hevia, Cristina Pujades, Miguel A. Valverde
Summary: Mechanical forces play a role in cytokinesis by activating the mechanosensitive Piezo1 channel at the intercellular bridge; inhibition of Piezo1 leads to multinucleation. Piezo1 generates Ca2+ signals to facilitate ALIX delivery to the intercellular bridge.
Article
Multidisciplinary Sciences
Shai Adar-Levor, Dikla Nachmias, Shani T. Gal-Oz, Yarden M. Jahn, Nadine Peyrieras, Assaf Zaritsky, Ramon Y. Birnbaum, Natalie Elia
Summary: Animal cytokinesis ends with the formation of intercellular bridges that connect sibling cells, ultimately resolved by abscission. In early zebrafish development, delayed abscission leads to interconnected cell clusters during rapid division cycles, while acceleration of abscission occurs during the midblastula transition (MBT). Components of the ESCRT machinery enriched at intercellular bridges post-MBT suggest a role in driving abscission and influencing cell behavior coordination during embryonic development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Rahul Kumar, Vincent Francis, Maria S. Ioannou, Adriana Aguila, Maleeha Khan, Emily Banks, Gopinath Kulasekaran, Peter S. McPherson
Summary: Cytokinesis is regulated by Rab proteins and guanine nucleotide exchange factors (GEFs). DENND2B, a protein associated with cancer and congenital disorders, acts as a GEF for Rab35 and plays a crucial role in cytokinetic abscission. Knockdown of DENND2B impairs actin depolymerization, delays abscission, and activates checkpoint proteins, leading to multinucleated cells and altered cytokinesis.
Article
Multidisciplinary Sciences
Janna Luessing, Chituru C. Okowa, Emer Brennan, Muriel Voisin, Noel F. Lowndes
Summary: This study reveals a cell cycle-specific role of ATR in abscission, which is important for genome stability. ATR and ATRIP localize to the midbody during late cytokinesis and regulate abscission independently of RPA or detectable ssDNA. The regulation of abscission by ATR requires the presence of CHMP4C, CDC7 kinase, and ETAA1.
Article
Biochemistry & Molecular Biology
Adrien Presle, Stephane Fremont, Audrey Salles, Pierre-Henri Commere, Nathalie Sassoon, Clarisse Berlioz-Torrent, Neetu Gupta-Rossi, Arnaud Echard
Summary: The midbody remnant (MBR) generated during cell division contains information that induces cancer cell proliferation, influences cell polarity, and promotes dorso-ventral axis specification. However, the mechanisms by which MBRs are captured by daughter cells or distant cells are poorly understood. A study found that BST2/tetherin, a restriction factor known for blocking the release of enveloped viruses, plays a role in retaining MBRs after cytokinesis. Knocking out BST2 leads to detachment of MBRs from cell surface and their transfer to distant cells, suggesting a new link between cytokinesis and viral biology.
Article
Plant Sciences
Jinhua Li, Ziqi Yan, Hongmei Li, Qiong Shi, Vidhula Ahire, Shenqiu Zhang, Naganna Nimishetti, Dun Yang, Thaddeus D. Allen, Jing Zhang
Summary: The study identified scoulerine as a compound capable of inducing mitotic and cytokinetic defects in human cancer cells through AURKB inhibition. Scoulerine may work upstream of the chromosomal passenger protein complex to reduce AURKB activity, making it a potential target for cancer therapy. The findings suggest that scoulerine or its analogues could be used as non-toxic components in combination therapies aimed at inhibiting the chromosomal passenger protein complex.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Pingping Dong, Ziqing Cai, Bingfeng Li, Yueqin Zhu, Alice K. Y. Chan, Michael W. L. Chiang, Chun Hang Au, Wing Kin Sung, Tan To Cheung, Chung Mau Lo, Kwan Man, Nikki P. Lee
Summary: The study reveals a novel function of HFE in promoting cytokinesis during mitotic cell division in HCC, independent of its iron-regulating ability. Clinical analysis shows that upregulation of HFE is associated with larger tumors and poor prognosis. The findings lay the groundwork for the development of tumor intolerable therapeutics.
Article
Cell Biology
Zachary McDargh, Tianyi Zhu, Hongkang Zhu, Ben O'Shaughnessy
Summary: This study used molecular simulations to investigate the role of cofilin in the contractile ring during cell division. The simulations revealed that the absence of cofilin led to an increase in ring tension, resulting in bridging instabilities. This research identified the critical role of cofilin in actomyosin turnover and its importance in protecting the contractile ring during cytokinesis.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biology
Dawn M. Wenzel, Douglas R. Mackay, Jack J. Skalicky, Elliott L. Paine, Matthew S. Miller, Katharine S. Ullman, Wesley Sundquist, Aurelien Roux
Summary: In this study, the interactions between human MIT domains and ESCRT-III tails were comprehensively mapped and quantified. Several new interactions were discovered, and the structure of the SPASTIN MIT domain in complex with the IST1 C-terminal tail was reported. The study also revealed the localization and functions of three MIT enzymes in cytokinetic abscission.
Review
Cell Biology
Joseph O. Magliozzi, James B. Moseley
Summary: Cell polarity signaling pathways play a direct role in regulating the contractile actomyosin ring (CAR) during cytokinesis, contributing to both local organization of CAR proteins and structures, as well as global organization of the division plane within a cell. Recent phosphoproteomics studies have identified new connections between cell polarity kinases and CAR proteins, pointing to conserved processes across different organisms. Opportunities for future research exist to further explore the conservation and divergence in the cell division process among organisms.
Article
Cell Biology
James Glover, Edward J. Scourfield, Leandro N. Ventimiglia, Xiaoping Yang, Steven Lynham, Monica Agromayor, Juan Martin-Serrano
Summary: This study reveals that UMAD1 is an important component in the process of cytokinetic abscission, facilitating the final stage of cell division by interacting with ESCRT-I.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Physical
Yunju Cho, Youhyun Nam, Hyung Ho Lee, Rakwoo Chang
Summary: Cytokinesis requires ALIX and CEP55 to activate cell abscission in somatic cells, while in germ cells, TEX14 forms intercellular bridges with CEP55, blocking cell abscission. These bridges play important roles in germ cell synchronization and organelle/molecule transfer. Understanding TEX14's function can provide insights into abscission inactivation and cancer cell proliferation inhibition.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Cell Biology
Benjamin Lacroix, Gaelle Letort, Laras Pitayu, Jeremy Salle, Marine Stefanutti, Gilliane Maton, Anne-Marie Ladouceur, Julie C. Canman, Paul S. Maddox, Amy S. Maddox, Nicolas Minc, Francois Nedelec, Julien Dumont
DEVELOPMENTAL CELL
(2018)
Editorial Material
Cell Biology
Julie C. Canman, Clemens Cabernard
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Biochemistry & Molecular Biology
Vanessa M. Hill, Reed M. O'Connor, Gunter B. Sissoko, Ifeoma S. Irobunda, Stephen Leong, Julie C. Canman, Nicholas Stavropoulos, Mimi Shirasu-Hiza
Article
Biology
Tim Davies, Han X. Kim, Natalia Romano Spica, Benjamin J. Lesea-Pringle, Julien Dumont, Mimi Shirasu-Hiza, Julie C. Canman
Article
Cell Biology
Jia Wang, Ekatherina Batourina, Kerry Schneider, Spenser Souza, Theresa Swayne, Chang Liu, Christopher D. George, Tiffany Tate, Hanbin Dan, Gregory Wiessner, Yelena Zhuravlev, Julie C. Canman, Indira U. Mysorekar, Cathy Lee Mendelsohn
Article
Biology
Frances Edwards, Gilliane Maton, Nelly Gareil, Julie C. Canman, Julien Dumont
Article
Biology
Rebecca Delventhal, Reed M. O'Connor, Meghan M. Pantalia, Matthew Ulgherait, Han X. Kim, Maylis K. Basturk, Julie C. Canman, Mimi Shirasu-Hiza
Article
Multidisciplinary Sciences
Matt Ulgherait, Anna Chen, Sophie F. McAllister, Han X. Kim, Rebecca Delyenthal, Charlotte R. Wayne, Christian J. Garcia, Yocelyn Recinos, Miles Oliva, Julie C. Canman, Martin Picard, Edward Owusu-Ansah, Mimi Shirasu-Hiza
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Sophia M. Hirsch, Frances Edwards, Mimi Shirasu-Hiza, Julien Dumont, Julie C. Canman
Summary: This study found that astral microtubules can support midbody assembly in the absence of a central spindle, and midbody assembly is more predictive of successful cytokinesis than central spindle assembly.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Matt Ulgherait, Adil M. Midoun, Scarlet J. Park, Jared A. Gatto, Samantha J. Tener, Julia Siewert, Naomi Klickstein, Julie C. Canman, William W. Ja, Mimi Shirasu-Hiza
Summary: Studies have shown that intermittent Time-restricted feeding (iTRF) extends the lifespan of fruit flies and delays aging markers, with circadian-regulated autophagy playing a crucial role in this process.
Article
Cell Biology
Etan R. Aber, Christopher J. Griffey, Tim Davies, Alice M. Li, Young Joo Yang, Katherine R. Croce, James E. Goldman, Jaime Grutzendler, Julie C. Canman, Ai Yamamoto
Summary: This study reveals that mature oligodendrocytes rely on macroautophagy to degrade myelin sheath and maintain its homeostasis, and disruption of this process can lead to neurodegenerative diseases and death.
Article
Biology
Nicolas Macaisne, Laura Bellutti, Kimberley Laband, Frances Edwards, Laras Pitayu-Nugroho, Alison Gervais, Thadshagine Ganeswaran, Helene Geoffroy, Gilliane Maton, Julie C. Canman, Benjamin Lacroix, Julien Dumont
Summary: During cell division, the microtubule-based spindle plays a crucial role in chromosome segregation. The interaction between spindle microtubules and kinetochores is essential for the proper alignment and segregation of chromosomes. This study focuses on a kinetochore module called the BHC module, which consists of BUB-1, HCP-1/2, and CLS-2, and shows that it stabilizes microtubules, promoting spindle formation and accurate chromosome segregation.
Meeting Abstract
Cell Biology
T. Davies, H. X. Kim, N. Romano Spica, B. J. Lesea-Pringle, J. Dumont, M. Shirasu-Hiza, J. C. Canman
MOLECULAR BIOLOGY OF THE CELL
(2018)
Meeting Abstract
Cell Biology
G. Maton, N. Gareil, J. C. Canman, J. Dumont
MOLECULAR BIOLOGY OF THE CELL
(2017)
Meeting Abstract
Cell Biology
S. Hirsch, S. Sundaramoorthy, Y. Zhuravlev, T. Davies, J. C. Waters, M. M. Shirasu-Hiza, J. Dumont, J. C. Canman
MOLECULAR BIOLOGY OF THE CELL
(2017)
