Journal
NEUROCHEMICAL RESEARCH
Volume 40, Issue 4, Pages 674-687Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-015-1514-4
Keywords
Stem cells; Neuronal differentiation; Src kinases; Sodium channels
Categories
Funding
- NIH [NS04 58710, NS057255, NS058710]
- American Heart Association
- [12GRNT12060222]
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Voltage-gated Na+ channel activity is vital for the proper function of excitable cells and has been indicated in nervous system development. Meanwhile, the Src family of non-receptor tyrosine kinases (SFKs) has been implicated in the regulation of Na+ channel activity. The present investigation tests the hypothesis that Src family kinases influence neuronal differentiation via a chronic regulation of Na+ channel functionality. In cultured mouse embryonic stem (ES) cells undergoing neural induction and terminal neuronal differentiation, SFKs showed distinct stage-specific expression patterns during the differentiation process. ES cell-derived neuronal cells expressed multiple voltage-gated Na+ channel proteins (Na-v) and underwent a gradual increase in Na+ channel activity. While acute inhibition of SFKs using the Src family inhibitor PP2 suppressed the Na+ current, chronic inhibition of SFKs during early neuronal differentiation of ES cells did not change Na-v expression. However, a long-lasting block of SFK significantly altered electrophysiological properties of the Na+ channels, shown as a right shift of the current-voltage relationship of the Na+ channels, and reduced the amplitude of Na+ currents recorded in drug-free solutions. Immunocytochemical staining of differentiated cells subjected to the chronic exposure of a SFK inhibitor, or the Na+ channel blocker tetrodotoxin, showed no changes in the number of NeuN-positive cells; however, both treatments significantly hindered neurite outgrowth. These findings suggest that SFKs not only modulate the Na+ channel activation acutely, but the tonic activity of SFKs is also critical for normal development of functional Na+ channels and neuronal differentiation or maturation of ES cells.
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