Journal
JOURNAL OF CARBOHYDRATE CHEMISTRY
Volume 31, Issue 4-6, Pages 466-503Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07328303.2012.683909
Keywords
Nanoporous gold; Self-assembled monolayer; 8-Mercaptooctyl alpha-D-mannopyranoside; Lectin; Concanavalin A; Electrochemical impedance spectroscopy; Atomic force microscopy; Scanning electron microscopy
Funding
- UM-St. Louis
- NIGMS [R01-GM090254]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM090254] Funding Source: NIH RePORTER
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We have prepared SAMs containing 8-mercaptooctyl alpha-D-mannopyranoside either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding, near 1:9 solution molar ratio of thiolated alpha-mannoside to n-octanethiol, whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated alpha-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers and to image the bound Con A protein. Supplemental materials are available for this article. Go to the publisher's online edition of Journal of Carbohydrate Chemistry to view the free supplemental file.
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