☆ 4.0 Article

Semisynthesis of Erythropoietin Analog Having Three Oligosaccharides

JOURNAL OF CARBOHYDRATE CHEMISTRY (2011)

Journal

JOURNAL OF CARBOHYDRATE CHEMISTRY

Volume 30, Issue 4-6, Pages 306-319

Publisher

TAYLOR & FRANCIS INC

DOI: 10.1080/07328303.2011.604570

Keywords

Erythropoietin; Glycopeptide; Native chemical ligation; Sialic acid

Categories

Biochemistry & Molecular Biology Chemistry, Organic

Funding

Japan Society for the Promotion of Science [17GS0420, B 20350081]
RIKEN, Japan

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Abstract

An erythropoietin (EPO) analog having three complex-type biantennary sialyloligosaccharides was prepared. The full-length EPO glycopeptide was prepared through native chemical ligation of a triglycosylated peptide-(alpha)thioester corresponding to EPO 1-32 amino acid residues, which was synthesized chemically, and a polypeptide corresponding to EPO 33-166 amino acid residues, which was prepared by E. coli expression. Oxidative folding of the full-length glycopolypeptide afforded the biologically active EPO analog.

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