Journal
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Volume 135, Issue 1, Pages 39-44Publisher
SPRINGER
DOI: 10.1007/s00432-008-0449-4
Keywords
Extrahepatic cholangiocarcinoma; Loss of heterozygosity; Real time reverse transcription-polymerase chain reaction Immunohistochemistry
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Funding
- Shanghai Natural Science Foundation
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Objectives WW domain-containing oxidoreductase ( WWOX) is a tumor suppressor gene that maps to the common fragile site FRA16D on chromosome 16q23.3-24.1. To investigate the role of the WWOX gene in the development of extrahepatic cholangiocarcinoma (ECC), 30 tissue samples of ECC were examined. Methods Loss of heterozygosity (LOH), real time reverse transcription PCR (RT-PCR), and immunohistochemistry were used to assess the role of WWOX in cholangiocarcinoma. Results LOH was observed in 50% of cases, loss of mRNA was observed in 46.67% of the cases and loss of WWOX protein expression was found in 56.67% of ECC tissue samples. LOH, mRNA, and protein expression had a significant correlation with histological grading. The correlation coefficients were -0.623, -0.475 and -0.543, respectively. However, WWOX expression had no correlations with other clinicopathological factors such as age, gender, clinical staging or the status of preoperative hepatic function ( p > 0.05). Poorly differentiated ECC had significantly lower expression of WWOX than that of moderately or well differentiated ones ( p < 0.05). Conclusions Loss of WWOX may be involved in the tumorigenesis of extrahepati cholangiocarcinoma.
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