Journal
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Volume 135, Issue 1, Pages 131-140Publisher
SPRINGER
DOI: 10.1007/s00432-008-0429-8
Keywords
Colon cancer; IL-4; PP2A; SHP-1; SOCS-1; Stat6
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Funding
- National Natural Science Foundation of China [30470981]
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Purpose To investigate potential differences in the expression of Stat6 regulatory genes that may influence IL-4/ Stat6 activities ( phenotypes) in colon cancer cells. Methods RT-PCR method was employed to examine the constitutive mRNA expression of Stat6 negative regulators SOCS-1 and SHP-1, and positive regulator PP2A in colon cancer cell lines HT-29 and Caco-2. Stat6 protein expression and nuclear phosphorylation were detected using Western blotting. Results Caco-2 cells carrying inactive Stat6(null) phenotype showed normal constitutive expression of Stat6 but decreased phosphorylation of nuclear Stat6 compared with HT-29 cells carrying active Stat6(high) phenotype. Stat6(null) Caco-2 cells expressed increased levels of mRNA and protein of SOCS-1 and SHP-1, and decreased mRNA expression of PPP2CA and PPP2CB, encoding two critical subunits of PP2A. Conclusions Constitutively increased expression of Stat6 negative regulators SOCS-1 and SHP-1, together with decreased expression of positive regulator PP2A, may play a role in forming the inactive Stat6(null) phenotype in colon cancer cells.
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