Article
Plant Sciences
Meiping Li, Dan Wang, Xiaofei Long, Zhaodong Hao, Ye Lu, Yanwei Zhou, Ye Peng, Tielong Cheng, Jisen Shi, Jinhui Chen
Summary: This study established and optimized a highly efficient genetic transformation system for Liriodendron hybrid using Agrobacterium-mediated transformation. By investigating various factors, the study successfully inserted functional genes into embryogenic calli and achieved plant regeneration and selection. The results demonstrated successful expression and integration of the inserted genes into the Liriodendron hybrid genome.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Article
Plant Sciences
Huimin Ren, Yan Xu, Xiaohong Zhao, Yan Zhang, Jamshaid Hussain, Fuqiang Cui, Guoning Qi, Shenkui Liu
Summary: Casuarina equisetifolia is widely used for agroforestry plantations for various purposes. This study established an efficient regeneration system using stem segment explants, optimizing callus induction and regeneration processes. Genetic transformation protocol using Agrobacterium tumefaciens was also optimized, leading to improved transformation efficiency to obtain transgenic plants in about 4 months. The findings will pave the way for further genetic manipulation and functional genomics of C. equisetifolia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Shengnan Liu, Yunlu Shi, Fang Liu, Yan Guo, Minhui Lu
Summary: This study presents an optimized transformation system that improves the infection and genetic transformation frequency of immature maize embryos mediated by Agrobacterium tumefaciens. The use of LaCl3 pretreatment enhances the infection frequency, increases the proportion of positive callus, yields more positive regenerated plantlets, and significantly increases the transformation frequency from 8.40% to 17.60%.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Xiaoping Wang, Shanshan Chen, Haonan Zhang, Ping Luo, Fangping Zhou, Bingshan Zeng, Jianmin Xu, Chunjie Fan
Summary: An efficient adventitious bud regeneration and transformation system of Eucalyptus was established using the genotype E. urophylla x E. grandis DH32-29, providing a powerful tool for genetic improvement and gene function analysis in Eucalyptus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Yuhuan Jin, Li Guo, Danqing Liu, Yongguang Li, Hao Ai, Xianzhong Huang
Summary: Translation: Establishment of tissue culture and efficient Agrobacterium-mediated genetic transformation in ephemeral plant Arabidopsis pumila for studying adaptation mechanisms to desert environments. Callus tissue formation was induced on different parts of the plant, and multiple adventitious shoots were obtained. The highest callus induction rate was observed in petiole explants. Using Agrobacterium tumefaciens GV3101-mediated transformation, the target gene ApP5CS1.1 was found to be overexpressed in the transgenic plants. These protocols provide a research method for studying the cellular-level adaptive mechanisms of A. pumila to desert environments.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Agricultural Engineering
Yanfang Wu, Na Zhou, Xingnan Ni, Charles Obinwanne Okoye, Yongli Wang, Xia Li, Lu Gao, Gongke Zhou, Jianxiong Jiang
Summary: The study successfully obtained a high percentage of embryogenic calli from different genotypes of Miscanthus sinensis using mature seeds as explants, and developed a novel selective culture strategy to improve the efficiency of selecting resistant transformants. By optimizing the co-cultivation method and duration, the transformation frequency was significantly increased, providing an efficient tool for cultivar improvement and genetic analysis of Miscanthus sinensis or other Miscanthus species.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Michelle M. M. Dominguez, Carmen S. S. Padilla, Kranthi K. K. Mandadi
Summary: Developing an efficient transformation system is crucial for genetically engineering recalcitrant crops, especially trees. In this study, we present a stable plant transformation method for citrus genetic engineering using Agrobacterium tumefaciens. The methodology has been optimized for fourteen citrus varieties and includes infection, co-cultivation, selection, and culture media conditions. The transformation efficiency can reach 11%, and transgenic plants can be obtained in as early as six months, depending on the variety. The incorporation of helper virulence genes and antioxidants improves the transformation efficiency of specific recalcitrant cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Valentina Maggini, Priscilla Bettini, Fabio Firenzuoli, Patrizia Bogani
Summary: An efficient transformation protocol has been established for A. oleracea using Agrobacterium tumefaciens LBA4404 strain bearing the binary vector pBI121. Plant genetic transformation has been verified by direct polymerase chain reaction and GUS assay. The transformation efficiency has been affected by the high level of kanamycin as the selection agent.
Article
Plant Sciences
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao
Summary: This study developed a regeneration system for Chinese cabbage and established a simple and efficient genetic transformation method. The method can obtain transformants in a relatively short period of time, paving the way for further gene editing and functional studies.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Agricultural Engineering
Wenmai Mao, Huiyun Song, Yue Li, Yueyang Wang, Huijuan Lin, Chi Yao, Wei Zhou, Bo Yang, Xiaoyang Chen, Pei Li
Summary: Brazilian mahogany has significant economic value for its high-quality materials and medicines, but its growth and sustainable development are limited by environmental changes, poor natural regeneration ability, and excessive logging. This study successfully established a highly efficient regeneration and genetic transformation system using sterile seedling leaves from a good provenance, with T1 medium showing promising results for callus formation and adventitious bud production.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Review
Plant Sciences
Aparajita Choudhury, Manchikatla Rajam
Summary: Genetic transformation in legume plants is an important area of agricultural research. Despite some progress in improving crop yields, challenges such as recalcitrance and genotype-dependence still exist. Future research may focus on overcoming these obstacles and further increasing yields.
PLANT CELL REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Phanikanth Jogam, Dulam Sandhya, Anshu Alok, Mahipal S. Shekhawat, Venkataiah Peddaboina, Kashmir Singh, Venkateswar Rao Allini
Summary: A reliable and stable Agrobacterium-mediated genetic transformation system was developed for Artemisia pallens using cell suspension cultures. Attached cotyledon-derived callus was suitable for initiating suspension cultures, and heat shock treatment increased the transformation efficiency.
Article
Forestry
Shaofei Dang, Lifeng Zhang, Suying Han, Liwang Qi
Summary: A simple and efficient protocol for the genetic transformation of Japanese larch was developed and multiple transgenic cell lines were obtained. However, the expression levels of exogenous genes in the transgenic system were relatively low, possibly due to the large genome of Japanese larch.
Article
Plant Sciences
Nikhil Mehta, Aparna Singh, Raman Saini
Summary: This study standardized the parameters affecting regeneration and transformation using shoot apices in Vigna umbellata. The results showed that by priming the explants with 6-benzylaminopurine (BAP) and optimizing the concentration of growth regulators and antioxidants, efficient shoot formation and successful transformation could be achieved.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)