Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Immunology
Xia Luo, Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu, Ningqiu Li
Summary: In this study, a suspension culture system of Chinese perch brain cell (CPB) was developed for virus vaccine production, utilizing Cytodex 1 microcarrier in a stirred bioreactor.
Article
Biotechnology & Applied Microbiology
Hyoungki Kim, Soonjo Kwon
Summary: The study demonstrates that a hypoxic environment, created using specific chemicals in a suspension culture, enhances the proliferation of mouse clonal mesenchymal stem cells and promotes their differentiation into osteogenic cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Anatomy & Morphology
Masoud Soleimani, Ameneh Salimiyan Naghani
Summary: Hematopoietic stem cell transplantation is a cell-based therapy for hematological disorders, but finding proper donors is challenging. Generating these cells from induced pluripotent stem cells (iPSs) provides a fascinating and endless source. This study focused on mimicking the hematopoietic niche to generate HSCs from iPSs, with dynamic culture conditions proving to be more suitable than static ones. The use of a 3D culture bioreactor with DBM scaffold could enhance the differentiation of iPSs into HSCs and mimic the bone marrow niche.
Article
Biotechnology & Applied Microbiology
Richard Jeske, Shaquille Lewis, Ang-Chen Tsai, Kevin Sanders, Chang Liu, Xuegang Yuan, Yan Li
Summary: This study demonstrates a three-fold expansion of hMSCs in a microcarrier-based suspension bioreactor over seven days, resulting in smaller cell size, higher levels of ROS and Sirtuin-3. The study also shows increased Prostaglandin E-2 secretion and reduced Indoleamine-pyrrole 2,3-dioxygenase secretion in hMSCs in the bioreactor compared to static culture, indicating potential physiological alterations impacted by the bioreactor microenvironment during scalable production.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Materials Science, Biomaterials
Chunhua Luo, Dongyuan Lu, Lu Zheng, Fan Zhang, Xiao Zhang, Shouqin Lu, Chen Zhang, Xiaohua Jia, Xinyu Shu, Peiwen Li, Zhan Li, Mian Long
Summary: Mechanical cues in the form of substrate stiffness and topography play crucial roles in directing the differentiation of human embryonic stem cells into liver cells, improving both efficiency and functionality in liver-specific functions.
BIOMATERIALS SCIENCE
(2021)
Article
Polymer Science
Rachel Lev, Orit Bar-Am, Yoni Lati, Ombretta Guardiola, Gabriella Minchiotti, Dror Seliktar
Summary: This study explores the use of a 3D hydrogel microcarrier for suspention culture of HEK293 cells and recombinant Cripto-1 protein production. The yield of purified Cripto-1 obtained using the 3D microcarriers was significantly higher than that obtained with a 2D culture system. The bioactivity of the 3D-produced Cripto-1 was equivalent to commercially available Cripto-1 in various assays.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Cell & Tissue Engineering
Yizhe Song, Xiaoqian Chen, Decan Liang, Jing Liu, Jingqiu Li, Zhensheng Ou, Tingting Tang, Peiwen Xing, Leilei Guo, Shidu Zhang, Qunrui Ye, Wenjia Li, Yinghua Chen, Xiuli Wang
Summary: A novel 48-well dynamic suspension culture system was developed for human embryonic stem cells (hESCs), maintaining viability, pluripotency, and enabling differentiation into pancreatic beta like cells. This system may serve as an efficient platform for high-throughput drug screening.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Cell Biology
Pouria Fattahi, Jose M. de Hoyos-Vega, Jong Hoon Choi, Caden D. Duffy, Alan M. Gonzalez-Suarez, Yuji Ishida, Kianna M. Nguyen, Kihak Gwon, Quinn P. Peterson, Takeshi Saito, Gulnaz Stybayeva, Alexander Revzin
Summary: This study presents a two-compartment microfluidic device for co-cultivation of adult human hepatocytes and stem cells. The device allows the adult hepatocytes to remain highly functional and serve as a source of paracrine cues to drive hepatic differentiation of the neighboring stem cells. The microfluidic co-culture system improves the efficiency of stem cell differentiation compared to conventional methods.
Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Review
Biotechnology & Applied Microbiology
Ang-Chen Tsai, Christina A. Pacak
Summary: This article summarizes key steps and important bioreactor parameters involved in the planar culture to microcarrier hMSC manufacturing scheme.
BIOENGINEERING-BASEL
(2021)
Article
Pharmacology & Pharmacy
Yoshiki Ando, Fei-Chien Chang, Matthew James, Yang Zhou, Miqin Zhang
Summary: In this study, three-dimensional chitosan scaffolds are developed as microcarriers for the prolonged expansion of human neural stem cells (hNSCs) in bioreactors. The chitosan scaffolds exhibit desirable microstructural characteristics and mechanical properties, allowing for hNSC expansion in dynamic conditions for up to three weeks. This microcarrier approach has the potential for mass production of hNSCs in a three-dimensional setting.
Article
Oncology
Sheng-Xiang Yang, Jing-Fang Xiao, Ting-Kai Liu, Ze-Diao Huang, Xiang Li, Ye-Miao Chen, Xiao-Long Yang
Correction
Cell & Tissue Engineering
Kim Vanuytsel, Qing Cai, Satish Khurana, Swati Shetty, Joris R. Vermeesch, Laura Ordovas, Catherine M. Verfaillie
STEM CELL RESEARCH
(2020)
Review
Genetics & Heredity
Roya Ramezankhani, Neda Minaei, Mahnaz Haddadi, Shukoofeh Torabi, Mahdi Hesaraki, Hamed Mirzaei, Massoud Vosough, Catherine M. Verfaillie
Summary: Gene correction is a promising strategy to treat genetic diseases, with genome editing tools like CRISPR/Cas9 considered novel technologies capable of correcting genetic aberrations. While the increasing number of clinical trials show encouraging outcomes, challenges remain regarding safety and targeted delivery.
Article
Agriculture, Dairy & Animal Science
Laura Garcia-Mendivil, Diego R. Mediano, Adelaida Hernaiz, David Sanz-Rubio, Francisco J. Vazquez, Belen Marin, Oscar Lopez-Perez, Alicia Otero, Juan J. Badiola, Pilar Zaragoza, Laura Ordovas, Rosa Bolea, Inmaculada Martin-Burriel
Summary: This study demonstrates for the first time how ovine mesenchymal stem cells react to scrapie prion infection in vitro and how the differentiation into neuron-like cells increases their susceptibility to prion infection. The results indicate that in neurogenic conditions, ovine MSCs maintain detectable levels of PrPSc and the signal increases progressively over time, while in growth conditions, the PrPSc signal weakens and is lost over time.
Article
Cell Biology
Estel Ramos-Marques, Laura Garcia-Mendivil, Maria Perez-Zabalza, Hazel Santander-Badules, Sabarathinam Srinivasan, Juan Carlos Oliveros, Rafael Torres-Perez, Alberto Cebollada, Jose Maria Vallejo-Gil, Pedro Carlos Fresneda-Roldan, Javier Fananas-Mastral, Manuel Vazquez-Sancho, Marta Matamala-Adell, Juan Fernando Sorribas-Berjon, Javier Andre Bellido-Morales, Francisco Javier Mancebon-Sierra, Alexander Sebastian Vaca-Nunez, Carlos Ballester-Cuenca, Manuel Jimenez-Navarro, Jose Manuel Villaescusa, Elisa Garrido-Huescar, Margarita Segovia-Roldan, Aida Olivan-Viguera, Carlos Gomez-Gonzalez, Gorka Muniz, Emiliano Diez, Laura Ordovas, Esther Pueyo
Summary: This study characterized the transcriptome dynamics of aging left ventricle (LV) in humans, demonstrating the higher sensitivity of biological age (BA) over chronological age (CA) in explaining cardiac dysfunction. The study identified BA-related microRNAs and revealed depleted cardiac-specific processes undetected by CA. Furthermore, a microRNA-gene regulatory network related to cardiac processes was described, showing novel insights into human LV biological aging.
Article
Multidisciplinary Sciences
Laura Garcia-Mendivil, Maria Perez-Zabalza, Konstantinos Mountris, Sam Duwe, Nick Smisdom, Marta Perez, Lluis Lujan, Esther Wolfs, Ronald B. Driesen, Jose Maria Vallejo-Gil, Pedro Carlos Fresneda-Roldan, Javier Fananas-Mastral, Manuel Vazquez-Sancho, Marta Matamala-Adell, Juan Fernando Sorribas-Berjon, Javier Andre Bellido-Morales, Francisco Javier Mancebon-Sierra, Alexander Sebastian Vaca-Nunez, Carlos Ballester-Cuenca, Aida Olivan-Viguera, Emiliano Diez, Laura Ordovas, Esther Pueyo
Summary: Age-related fibrosis in the left ventricle (LV) increases with age, and is correlated with the biological age marker lipofuscin. Fibrosis modulates arrhythmia vulnerability and distribution should be considered for risk assessment.
Article
Engineering, Biomedical
Jingfang Xiao, Lijuan Zeng, Shuaishuai Ding, Yemiao Chen, Xiao Zhang, Xiu-wu Bian, Gan Tian
Summary: Nanoparticles lack specificity to tumors, however, mesenchymal stem cells can act as delivery vehicles for nanoparticles to enhance the treatment effect. In this study, bismuth selenide nanoparticles loaded adipose-derived mesenchymal stem cells were developed for targeted radiotherapy of non-small cell lung cancer. The results demonstrate the feasibility and potential of this tumor-targeting system for radiotherapy.
ADVANCED HEALTHCARE MATERIALS
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
N. Hernandez-Bellido, A. Postigo, C. Orrite, E. Garrido-Huescar, L. Garcia-Mendivil, E. Pueyo, S. Hernandez-Ainsa, L. Ordovas
CARDIOVASCULAR RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
E. Garrido-Huescar, L. Ordovas, E. Pueyo
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Julian Krauskopf, Kristel Eggermont, Rodrigo Furtado Madeiro Da Costa, Sacha Bohler, Duncan Hauser, Florian Caiment, Theo M. de Kok, Catherine Verfaillie, Jos C. Kleinjans
Summary: Parkinson's disease is a neurodegenerative disease with both genetic and sporadic cases, affecting a significant portion of the elderly population. Induced pluripotent stem cells can be used to derive dopaminergic neurons for in vitro disease modeling and studying the pathomolecular mechanisms of PD.
MOLECULAR PSYCHIATRY
(2022)
Article
Agriculture, Dairy & Animal Science
Marta Moya-Jodar, Giulia Coppiello, Juan Roberto Rodriguez-Madoz, Gloria Abizanda, Paula Barlabe, Amaia Vilas-Zornoza, Asier Ullate-Agote, Chiara Luongo, Ernesto Rodriguez-Tobon, Sergio Navarro-Serna, Evelyne Paris-Oller, Maria Oficialdegui, Xonia Carvajal-Vergara, Laura Ordovas, Felipe Prosper, Francisco Alberto Garcia-Vazquez, Xabier L. Aranguren
Summary: The generation of organs using pluripotent stem cells combined with genetically engineered pig embryos through blastocyst complementation could provide a solution to the worldwide shortage of transplantable organs. In this study, a protocol to create vascular-disabled pig embryos using the CRISPR/Cas9 system was optimized, which could contribute to the generation of rejection-free humanized organs in pigs.
Article
Pharmacology & Pharmacy
Jingfang Xiao, Qinghua Ma, Ruili Cai, Jingya Miao, Zexuan Yan, Xiaolong Yang, Yemiao Chen
Summary: This study demonstrated the potential of extracts obtained from Chaetomium cochliodes and Penicillium sp. for tumor treatment, showing inhibitory effects on tumor cell growth and induction of apoptosis. In vivo experiments also showed that these extracts significantly reduced the volume and size of subcutaneous transplanted tumors.
INTERNATIONAL JOURNAL OF PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Sergei Grebenyuk, Abdel Rahman Abdel Fattah, Manoj Kumar, Burak Toprakhisar, Gregorius Rustandi, Anja Vananroye, Idris Salmon, Catherine Verfaillie, Mark Grillo, Adrian Ranga
Summary: By developing micro-perfused 2-photon printing of 3D microfluidics, the authors have successfully engineered large-scale, viable and functional neural and hepatic 3D tissues. They have achieved the perfusion of multi-mm(3) tissue constructs by generating networks of synthetic capillary-scale 3D vessels, addressing the challenge of vascularization in regenerative medicine.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Samantha Zaunz, Jonathan De Smedt, Lukas Lauwereins, Lana Cleuren, Charlie Laffeber, Manmohan Bajaj, Joyce H. G. Lebbink, Jurgen A. Marteijn, Kim De Keersmaecker, Catherine Verfaillie
Summary: APEX1 plays a crucial role in adult regenerative hematopoiesis, with its nuclease and redox domains impacting proliferating HSPCs differently.
STEM CELL REVIEWS AND REPORTS
(2023)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Maria Perez-Zabalza, Laura Garcia-Mendivil, Kostantinos A. Mountris, Nick Smisdom, Jose M. Vallejo-Gil, Pedro C. Fresneda-Roldan, Javier Fananas-Mastral, Marta Matamala-Adell, Fernando Sorribas-Berjon, Manuel Vazquez-Sancho, Javier Andre Bellido-Morales, Francisco Javier Mancebon-Sierra, Alexander Sebastian Vaca-Nunez, Carlos Ballester-Cuenca, Aida Olivan-Viguera, Laura Ordovas, Esther Pueyo
Summary: This study quantified the amount and spatial organization of collagen in the human left ventricle, showing an increase in collagen amount and clustering with age. Fibrosis induced a decrease in conduction velocity, while increased clustering did not impact conduction velocity. In terms of ventricular repolarization, an increase in fibrosis percentage led to a reduction in action potential duration.
2021 COMPUTING IN CARDIOLOGY (CINC)
(2021)