Journal
JOURNAL OF BIOTECHNOLOGY
Volume 182, Issue -, Pages 54-60Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2014.04.017
Keywords
Cyanobacteria; Biofuels; Tolerance; Reactive oxygen species; Transcription factors
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Funding
- Swedish research council Formas
- Knut and Alice Wallenberg Foundation
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Among phenotypes of interest for an industrial cyanobacteria host are improved tolerance to temperature, salt, and solvent stress. Cellular responses to many stresses are controlled by the network of sensory receptors and downstream regulatory proteins. We applied transcription factor engineering to Synechocystis and tested mutant strains for tolerance to temperature and the biofuel 1-butanol. Histidine kinases (Hik), response regulators (Rre), and an RNA polymerase sigma factor (SigB) were overexpressed or deleted. Overexpression of SigB increased both temperature and butanol tolerance and lowered the intracellular concentration of reactive oxygen species. This report demonstrates that alteration of regulatory proteins in a cyanobacterium can be a useful tool to improve stress tolerance. (C) 2014 Elsevier B.V. All rights reserved.
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