Journal
JOURNAL OF BIOTECHNOLOGY
Volume 167, Issue 2, Pages 85-93Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2013.02.005
Keywords
Compatible solute; Ectoine; 5-Hydroxyectoine; Hansenula polymorpha; Whole-cell biocatalysis; Halomonas elongata
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Funding
- German National Ministry of Education and Research (BMBF)
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Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to mainly contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition the cell's essential functions are maintained under stress conditions like high salinity, heat or aridity stress. Hansenula polymorpha was engineered to catalyze the transformation of monomeric substrates to 5-hydroxyectoine. For this purpose four genes encoding the enzymes of the 5-hydroxyectoine biosynthesis pathway of Halomonas elongata, EctA, EctB, EctC, and EctD, were inserted into the genome of H. polymorpha. Subsequently the syntheses of ectoine and 5-hydroxyectoine were analyzed and optimized. We showed that H. polymorpha is a suitable system for recombinant 5-hydroxyectoine synthesis in gram per liter scale (2.8 g L-1 culture supernatant, 365 mu mol/g dcw) in which almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity were achieved. (C) 2013 Elsevier B.V. All rights reserved.
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