Article
Microbiology
Arjun Chakrawal, Salvatore Calabrese, Anke M. Herrmann, Stefano Manzoni
Summary: This study presents a theoretical framework to quantify the microbial growth rate under conditions of C, N, and energy limitations. The research shows that the degree of reduction of organic matter, availability of electron acceptors, and different nitrogen sources together control the growth rate of microorganisms under C, nutrient, and energy-limited conditions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biodiversity Conservation
Chupei Shi, Carolina Urbina-Malo, Ye Tian, Jakob Heinzle, Steve Kwatcho Kengdo, Erich Inselsbacher, Werner Borken, Andreas Schindlbacher, Wolfgang Wanek
Summary: Increasing global temperatures accelerate soil carbon cycling and promote nitrogen and phosphorus dynamics in terrestrial ecosystems. Warming differentially affects ecosystem carbon, nitrogen, and phosphorus dynamics, intensifying imbalances between soil resources, plants, and soil microorganisms. Long-term soil warming shifts microbial element limitation from carbon to carbon-phosphorus co-limitation, with significant consequences for soil carbon, nitrogen, and phosphorus cycles under warming.
GLOBAL CHANGE BIOLOGY
(2023)
Article
Ecology
Michelle Y. Wong, Christopher Neill, Roxanne Marino, Divino Silverio, Robert W. Howarth
Summary: Biological nitrogen fixation in tropical forests is affected by atmospheric molybdenum inputs and soil weathering. Experimental additions of molybdenum and phosphorus increased their availability and soil pH, but did not consistently enhance biological nitrogen fixation rates. High nitrogen availability in the Amazon region likely suppresses biological nitrogen fixation.
Article
Ecology
Ji Liu, Linchuan Fang, Tianyi Qiu, Haijian Bing, Yongxing Cui, Jordi Sardans, Enzai Du, Ji Chen, Wenfeng Tan, Manuel Delgado-Baquerizo, Guiyao Zhou, Qingliang Cui, Josep Penuelas
Summary: This study investigated the patterns of plant-microbial N/P limitation in forests across China and found significant divergence between the two. The N/P limitation was disconnected in 42.6% of plant-microbial communities, with only 17.7% of N and 39.7% of P limitations consistent. The divergence was more evident at mid-latitudes, where plants were mainly N limited and microbes were mainly P limited. The findings were consistent with ecological stoichiometry and highlighted the importance of soil chemistry in driving the divergence.
FUNCTIONAL ECOLOGY
(2023)
Review
Agronomy
Ruzhen Wang, Jiayu Lu, Yong Jiang, Feike A. Dijkstra
Summary: In this study, the authors expanded the conceptual framework of carbon allocation for nutrient acquisition in plants by introducing a new parameter called carbon efficiency for nutrient acquisition (CENA). They found that CENA increases with higher nutrient availability, but reaches a plateau when the availability of one nutrient increases at the expense of another. The relationship between CENA and mycorrhizal plants may differ from non-mycorrhizal plants, with CENA potentially being higher in mycorrhizal plants under low nutrient availability. Additionally, the CENA of nitrogen-fixing plants is independent of soil nitrogen availability but increases with soil phosphorus availability. The researchers conclude that these updated frameworks provide a better understanding of how plants optimize belowground carbon allocation for nutrient acquisition under varying nutrient availability conditions.
Article
Microbiology
Catherine Bannon, Insa Rapp, Erin M. Bertrand
Summary: Nutrient co-limitation, known as the simultaneous limitation of productivity by two or more nutrients, is of great importance in marine microbial communities and biogeochemical cycles. Community interaction co-limitation (CIC) is a new category that takes into account the interactions within microbial communities, where one part of the community is limited by one nutrient that affects the production or transformation of another nutrient required by another part of the community. Understanding and identifying CIC mechanisms require an expanded toolset and the incorporation of processes and theories from microbial ecology and evolution. There are still data gaps that limit our understanding of CIC mechanisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Peter M. Vitousek, Jesse Bloom Bateman, Oliver A. Chadwick
Summary: The study used a simple toy model to evaluate the controls of biogeochemical patterns along a climate gradient, showing that water is the most important factor shaping these patterns, with nitrogen and phosphorus also playing important roles. The model simulated peaks in delta N-15 and total P in surface soil at intermediate levels of precipitation and water balance on the gradient.
Article
Environmental Sciences
S. K. Schmidt, B. W. Johnson, A. J. Solon, P. Sommers, J. L. Darcy, K. Vincent, L. Vimercati, A. G. Fountain, D. L. Porazinska
Summary: This study evaluated the dynamics and limitations of nutrients in cryoconite hole sediments in the McMurdo Dry Valleys of Antarctica using various biogeochemical methods. The results showed variations in nutrient concentrations and isotope signatures among different glaciers, with Taylor Glacier being the most oligotrophic and the others relatively nutrient-rich. Phosphorus was identified as the ultimate limiting nutrient across all three glaciers. The study provides a comprehensive framework for understanding the biogeochemical cycling of carbon, nitrogen, and phosphorus in the cryobiosphere and highlights the need for further experimental research on the relative controls of climate, microbes, and nutrients on the biogeochemistry of cryoconite holes and other cryospheric ecosystems.
Article
Biotechnology & Applied Microbiology
Giseli Swerts Rocha, Christopher C. Parrish, Evaldo L. G. Espindola
Summary: This study evaluated the effects of nitrogen limitation and cadmium exposure on the photosynthetic parameters and lipid classes of Ankistrodesmus densus. The results showed that both nitrogen and cadmium had significant impacts on algal growth, chlorophyll a production, and lipid composition. The combined stressors had synergistic effects on various parameters, indicating their influence on algal physiology. However, the algae's photoprotective mechanisms effectively prevented photodamage.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Katarzyna Wierzchowska, Bartlomiej Zieniuk, Dorota Nowak, Agata Fabiszewska
Summary: Microbial lipids are considered a sustainable alternative to traditional vegetable oils and have gained attention among researchers. This study investigated the impact of limiting inorganic phosphorus and nitrogen sources on cellular lipid biosynthesis in Y. lipolytica yeast, revealing a significant relationship between phosphorus concentration and lipid accumulation. Simultaneously limiting both phosphorus and nitrogen sources promoted lipid accumulation but hindered biomass growth, highlighting the importance of phosphorus as a factor in cultivating oleaginous microorganisms.
APPLIED SCIENCES-BASEL
(2021)
Article
Microbiology
Sophie Rabouille, Douglas A. Campbell, Takako Masuda, Tomas Zavrel, Gabor Bernat, Lubos Polerecky, Kimberly Halsey, Meri Eichner, Eva Kotabova, Susanne Stephan, Martin Lukes, Pascal Claquin, Jose Bonomi-Barufi, Ana Teresa Lombardi, Jan Cerveny, David J. Suggett, Mario Giordano, Jacco C. Kromkamp, Ondrej Prasil
Summary: Marine diazotrophs play key roles in biogeochemical fluxes, but their growth efficiency can be hindered by carbon and nitrogen limitations. The unicellular diazotrophic cyanobacterium Cyanothece faces a double burden of ATP-demanding nitrogen acquisition and metabolic losses when grown in obligate diazotrophy.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Pawlok Dass, Benjamin Z. Houlton, Yingping Wang, David Warlind, Scott Morford
Summary: The study shows that rock nitrogen weathering has a much greater impact on nitrogen-carbon interactions than anthropogenic nitrogen deposition. Rock nitrogen inputs and biological nitrogen fixation contribute more to terrestrial carbon uptake than anthropogenic nitrogen deposition, indicating that natural nitrogen inputs play a major role in terrestrial CO2 sequestration in Earth's ecosystems.
GLOBAL BIOGEOCHEMICAL CYCLES
(2021)
Article
Mechanics
Heng Wei, David A. Hutchins, Paul D. Ronney, Niema M. Pahlevan
Summary: This study proposes a growth model based on fluid dynamics to explore the formation and growth of bacterial colonies. The results show that the flow field significantly affects the colony growth rate and shape, with the Reynolds number playing a crucial role in shaping the colonies.
Article
Biotechnology & Applied Microbiology
Juan F. Ortiz-Medina, Mark R. Poole, Amy M. Grunden, Douglas F. Call
Summary: The anode potential has a significant impact on nitrogen gas fixation rates and ammonium production in Geobacter sulfurreducens. Low anode potential results in increased nitrogen fixation activity and higher intracellular concentrations of organic compounds, contributing to improved energy generation and use in microbial electrochemical technologies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Paul M. D'Agostino, Anna C. Y. Yeung, Anne Poljak, Trevor David Waite, Brett A. Neilan
Summary: This study compares the proteomic responses of different toxic and non-toxic strains of Raphidiopsis raciborskii under reduced iron concentrations, revealing that the toxic strain is more competitive during the exponential growth phase, while the non-toxic strain shows reduced protein expression in multiple primary metabolism pathways.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Maria A. Sinetova, Roman A. Sidorov, Anastasiya A. Medvedeva, Alexander Y. Starikov, Alexandra G. Markelova, Suleyman I. Allakhverdiev, Dmitry A. Los
Summary: The eustigmatophycean alga IPPAS H-242 possesses good growth characteristics and high content of the long chain polyunsaturated eicosapentaenoic fatty acid (EPA). Under salt stress, biomass accumulation and cell division were severely inhibited, with cells showing larger size, higher chloroplast volume, more proteins, and higher EPA proportion in lipids. Despite the increase in EPA proportion in total FA, salt stress leads to a decrease in total EPA productivity.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Microbiology
Lubos Polerecky, Takako Masuda, Meri Eichner, Sophie Rabouille, Marie Vancova, Michiel V. M. Kienhuis, Gabor Bernat, Jose Bonomi-Barufi, Douglas Andrew Campbell, Pascal Claquin, Jan Cerveny, Mario Giordano, Eva Kotabova, Jacco Kromkamp, Ana Teresa Lombardi, Martin Lukes, Ondrej Prasil, Susanne Stephan, David Suggett, Tomas Zavrel, Kimberly H. Halsey
Summary: Unicellular nitrogen fixing cyanobacteria (UCYN) are abundant in marine phytoplankton communities and can shift their strategies of intracellular N and C allocation under different N availabilities. This study revealed detailed mechanisms in Cyanothece for C and N management, including subpopulations fixing nitrogen at different times and the impact of external and internal nitrogen assimilation sources on cyanophycin synthesis. The prioritization of cyanophycin synthesis when N assimilation rates are highest was observed, along with the bypassing of cyanophycin synthesis in favor of protein synthesis once a cyanophycin quota is met.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Elena Kupriyanova, Maria A. Sinetova, Anna Leusenko, Alexander S. Voronkov, Dmitry A. Los
Summary: This study expressed different forms of EcaA protein in Escherichia coli and found that the recombinant proteins with a leader peptide could form mature EcaA in the outer layers of the cells, showing high extracellular carbonic anhydrase activity. The cyanobacterial signal peptide played an important role in the secretion machinery of E. coli.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Marine & Freshwater Biology
Elena Krivina, Anna Temraleeva, Maria Sinetova
Summary: The study presented in the article identifies newly isolated strains from a lake in Russia as a new species Micractinium kostikovii through morphological, molecular, and environmental characteristics analysis. The research highlights the importance of molecular tools in revolutionizing the study of algal diversity.
PHYCOLOGICAL RESEARCH
(2022)
Correction
Biochemistry & Molecular Biology
Keisuke Inomura, Takako Masuda, Meri Eichner, Sophie Rabouille, Tomas Zavrel, Jan Cerveny, Marie Vancova, Gabor Bernat, Gabrielle Armin, Pascal Claquin, Eva Kotabova, Susanne Stephan, David J. Suggett, Curtis Deutsch, Ondrej Prasil
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biology
David A. Gabrielyan, Boris Gabel, Maria A. Sinetova, Alexander K. Gabrielian, Alexandra G. Markelova, Natalia Shcherbakova, Dmitry A. Los
Summary: Microalgae are being increasingly utilized for carbon dioxide capture and conversion into valuable compounds. This study investigated the productivity of Chlorella sorokiniana IPPAS C-1 under different CO2 concentrations and ventilation coefficients. The results provide insights for improving the efficiency of intensive cultivation of microalgae.
Article
Biology
David A. Gabrielyan, Maria A. Sinetova, Boris Gabel, Alexander K. Gabrielian, Alexandra G. Markelova, Margarita Rodionova, Vladimir S. Bedbenov, Natalia Shcherbakova, Dmitry A. Los
Summary: This paper presents the growth characteristics of the microalgae Chlorella sorokiniana IPPAS C-1 in a specially designed cultivation system. The results show a significant increase in biomass through three-stage scale-up cultivation. The key parameters of the photobioreactors are also discussed.
Article
Chemistry, Physical
Ayshat M. Bozieva, Makhmadyusuf Kh. Khasimov, Roman A. Voloshin, Maria A. Sinetova, Elena V. Kupriyanova, Sergey K. Zharmukhamedov, Dmitry O. Dunikov, Anatoly A. Tsygankov, Tatsuya Tomo, Suleyman I. Allakhverdiev
Summary: This study aims to investigate the H2-releasing ability of new strains of cyanobacteria and evaluate the effects of the photosystem II inhibitor DCMU on H2 production under light and dark conditions. The results demonstrate that cultures treated with DCMU produced several times more H2 than untreated cells. The highest H2 photoproduction rate of 4.24 mmol H2 (mg Chl a h)-1 was observed in Dolichospermum sp. IPPAS B.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Agricultural Engineering
Miroslav Rebej, Tomas Jurena, Jiri Vondal, David Fuente Herraiz, Jan Cerveny, Zdenek Jegla
Summary: This paper presents the use of PIV technique to measure velocity fields in a stirred photobioreactor and validate a CFD model. The study includes grid independence analysis, velocity profiles, and shear stresses for different operating conditions. The results show good correlation between the numerical model and experimental data, but uncertainties exist in PIV measurements for bubbly flows.
BIOSYSTEMS ENGINEERING
(2023)
Article
Plant Sciences
D. A. Gabrielyan, M. A. Sinetova, A. K. Gabrielyan, L. A. Bobrovnikova, V. S. Bedbenov, A. Yu. Starikov, A. A. Zorina, B. V. Gabel, D. A. Los
Summary: This paper presents a laboratory system for intensive cultivation of microalgae and cyanobacteria, and showcases the results of experiments conducted using this system.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Elena Krivina, Maria Sinetova, Tatyana Savchenko, Evgeniy Degtyaryov, Elizaveta Tebina, Anna Temraleeva
Summary: The study on Micractinium genus species diversity is still relevant due to its simple morphology and high phenotypic plasticity. These studies have practical significance as some of these species have high biotechnological potential, capable of accumulating valuable metabolites, including polyunsaturated fatty acids. The studied strains were identified as two new species, M. lacustre and M. thermotolerans, based on morphological characteristics, habitat, phylogenetic analysis, and fatty acids profile.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biodiversity Conservation
Elena S. Krivina, Lidia A. Bobrovnikova, Anna D. Temraleeva, Alexandra G. Markelova, David A. Gabrielyan, Maria A. Sinetova
Summary: Despite the large number of publications about Chlorella-like algae, their identification remains challenging due to their simplicity and ability to change their appearance. This study uses molecular techniques to understand the diversity of these algae and proposes a new genus, Neochlorella, with IPPAS C-1210 as the type species. Additionally, the study reassigns the strain C. thermophila ITBB HTA 1-65 to this new genus. The study also discusses the unique ultrastructural and physiological traits of the new species.
Review
Biology
Natalya Yuorieva, Maria Sinetova, Ekaterina Messineva, Irina Kulichenko, Artem Fomenkov, Olga Vysotskaya, Ekaterina Osipova, Angela Baikalova, Olga Prudnikova, Maria Titova, Alexander V. V. Nosov, Elena Popova
Summary: This review focuses on six genetic collections of algae, cyanobacteria, and plant materials maintained at the Institute of Plant Physiology of the Russian Academy of Sciences. These collections have been preserved since the 1950-1970s using in vitro and cryopreservation techniques. They play a significant role in research, biotechnological interventions, commercial application, and biodiversity conservation, and serve as a genetic basis for green biotechnologies.
Article
Agricultural Engineering
Joao S. Rodrigues, Laszlo Kovacs, Martin Lukes, Rune Hoper, Ralf Steuer, Jan Cerveny, Pia Lindberg, Tomas Zavrel
Summary: Engineering cyanobacteria for isoprene production has attracted attention in biotechnology. This study focuses on optimizing cultivation conditions. Results show that isoprene production increases under violet light and at elevated temperature. The increased thermotolerance in the isoprene producer is attributed to the physical presence of isoprene. This opens new possibilities for sustainable bio-production of isoprene and other products.
BIORESOURCE TECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Diana Buzova, Maria Rita Braghini, Salvatore Daniele Bianco, Oriana Lo Re, Marco Raffaele, Jan Frohlich, Antoniya Kisheva, Annalisa Crudele, Antonella Mosca, Maria Rita Sartorelli, Clara Balsano, Jan Cerveny, Tommaso Mazza, Anna Alisi, Manlio Vinciguerra
Summary: This study identified enrichment of histone macroH2A1.2 in the plasma of children with NAFLD, and a significant correlation between cfDNA methylation levels and NASH. The combination of cfDNA methylation, PNPLA3 rs738409 variant, and biochemical markers can strongly predict the progression of pediatric NAFLD to NASH.
HEPATOLOGY COMMUNICATIONS
(2022)