Article
Biochemical Research Methods
Yusuke Shibafuji, Nobuyoshi Nagao, Masafumi Yohda
Summary: Multi-omics analysis compared the effects of adding Tyr and Cystine in CHO cells. Adding Cystine resulted in decreased viability and productivity due to ER stress, ERAD, and apoptosis. On the other hand, adding Tyr suppressed ER stress and apoptosis by increasing ubiquinone biosynthesis. Simultaneously adding Tyr with Cystine improved growth, viability, and mAb productivity by activating GSH metabolism, suppressing ER stress and oxidative stress, reducing ERAD, and activating the tricarboxylic acid cycle.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Cell Biology
Chuan Zhang, Lu Wang, Chi Jin, Jiahui Zhou, Chaofan Peng, Yong Wang, Ziwei Xu, Dongsheng Zhang, Yuanjian Huang, Yue Zhang, Dongjian Ji, Wen Peng, Kangpeng Jin, Junwei Tang, Yifei Feng, Yueming Sun
Summary: Colorectal cancer is a common cancer worldwide, with liver metastasis being a key factor affecting prognosis. A study revealed that Lnc-LALC can regulate CRC cell metastasis ability by affecting DNA methylation status, highlighting its important role in CRLM.
CELL DEATH & DISEASE
(2021)
Review
Biochemical Research Methods
James Donaldson, Dirk-Jan Kleinjan, Susan Rosser
Summary: Fed-batch culture of Chinese hamster ovary (CHO) cells is the most commonly used method for producing biopharmaceuticals. Static CHO cell-line engineering has improved productivity and quality, but conditions change during fed-batch culture. Dynamic engineering strategies can optimize traits by implementing synthetic sense-and-respond programs.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Virology
Liwei Li, Sina Qiao, Shumao Wang, Jiachen Liu, Kuan Zhao, Yanjun Zhou, Guoxin Li, Yifeng Jiang, Changlong Liu, Guangzhi Tong, Wu Tong, Fei Gao
Summary: This study successfully expressed ASFV p17 in CHO cells and generated a specific antibody against p17. The identified linear epitope of the antibody was conservative among different ASFV strains, indicating its potential for efficient detection and promising application prospects in future ASF vaccine research.
Article
Plant Sciences
Daniel Matsusaka, Javier F. Botto, Diego H. Sanchez
Summary: This study reveals the central regulatory role of specific noncoding tandem repeats in the intersection between epigenetic silencing and gene expression, and their importance in plant stress responses.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Biotechnology & Applied Microbiology
Kexue Liang, Hongzhen Luo, Qi Li
Summary: In this study, the effects of different perfusion culture strategies on process stability, product titer, and product quality were investigated. Continuous perfusion was found to be more effective in maintaining high levels of cell density, product titer, and quality compared to intermittent perfusion culture. Furthermore, the study optimized the perfusion culture conditions by adjusting perfusion rates and culture temperature. The optimized strategy resulted in significantly higher product titer and maintained excellent product quality.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yanjing Wang, Siyi Wang, Yuchen Niu, Buyong Ma, Jingjing Li
Summary: CXCL14 is a tumor suppressor gene in colorectal carcinoma that is initially activated and then silenced during tumor occurrence and deterioration. Its silencing is primarily caused by promoter hypermethylation. The methylation level of CXCL14 is associated with the anatomical site of tumor occurrence and is correlated with patient age and prognosis. Reversing the hypermethylation of CXCL14 may serve as an epigenetic therapy for colon cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Daai Zhang, Chengcheng Zhong, Neil A. Smith, Robert de Feyter, Ian K. Greaves, Steve M. Swain, Ren Zhang, Ming-Bo Wang
Summary: This study shows that nucleotide mismatches can prevent intrinsic self-silencing of hpRNA transgenes in plants.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Margaux Olivier, Amy Hesketh, Marie-Noelle Pouch-Pelissier, Thierry Pelissier, Ying Huang, David Latrasse, Moussa Benhamed, Olivier Mathieu
Summary: Transcriptional silencing is a crucial mechanism for gene expression control, and it is regulated by specific epigenetic marks on chromatin. This study demonstrates that the DNA helicase RTEL1 has an important role in transcriptional silencing. RTEL1 deficiency leads to upregulation of genes enriched in H3K27me3 mark, decreased DNA methylation, and hypermethylation and increased H3K27me3 in heterochromatin. Additionally, RTEL1 is involved in stabilizing DNA methylation and H3K27me3 patterns. These findings highlight the previously unappreciated roles of RTEL1 in transcriptional silencing.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xin Tong, Jia-Jia Zhao, Ya-Lan Feng, Jing-Ze Zou, Jian Ye, Junfeng Liu, Chenggui Han, Dawei Li, Xian-Bing Wang
Summary: Symptom recovery is achieved through a balanced arms race between viruses and plants. A small peptide called VISP1 can mediate the autophagic degradation of viral silencing suppressors, thus maintaining the balance between virus pathogenicity and plant immunity.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Kanako Kojima-Kita, Satomi Kuramochi-Miyagawa, Manabu Nakayama, Haruhiko Miyata, Steven E. Jacobsen, Masahito Ikawa, Haruhiko Koseki, Toru Nakano
Summary: The PIWI-interacting-RNA pathway plays a crucial role in repressing transposable element expression in mouse embryonic male germ cells via de novo DNA methylation, with proteins like MIWI2 and MORC3 involved in the process.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Rin Miyajima, Hitomi Manaka, Tatsuya Honda, Noritaka Hashii, Masato Suzuki, Masahiro Komeno, Koichi Takao, Akiko Ishii-Watabe, Kazuei Igarashi, Toshihiko Toida, Kyohei Higashi
Summary: The depletion of polyamines in CHO cells increases IgG galactosylation and induces ER stress related to glycoprotein folding, leading to growth arrest. Introducing polyamines, especially SPD, to serum-free CHO culture medium may contribute to consistent antibody production and quality control.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Megan Beetch, Cayla Boycott, Sadaf Harandi-Zadeh, Tony Yang, Benjamin J. E. Martin, Thomas Dixon-McDougall, Kevin Ren, Allison Gacad, John H. Dupuis, Melissa Ullmer, Katarzyna Lubecka, Rickey Y. Yada, Carolyn J. Brown, LeAnn J. Howe, Barbara Stefanska
Summary: The study investigated the dynamics between oncogenic TF OCT1 binding and de novo DNA methyltransferase DNMT3B binding in PTS-treated invasive breast cancer cells. It was found that PTS led to decreased OCT1 binding and increased DNMT3B binding in gene regulatory regions associated with oncogenic functions, resulting in gene methylation and silencing. Our findings provide insights into the mechanistic players and gene targets that contribute to the anticancer action of stilbenoid polyphenols.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Engineering, Chemical
Mei-Wei Lin, Chih-Che Shen, Yen-Ju Lin, Min-Yuan Chou, Nam Ngoc Pham, Yi-Hao Chang, Chin-Wei Chang, Jih Ru Hwu, Mai Thanh Thi Nguyen, Yu-Chen Hu
Summary: By utilizing the CRISPR-Cas13d system to silence endogenous genes controlling lactate production, fucosylation, and cell death, a CHO cell platform with improved IgG production in terms of quantity and quality was successfully developed.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2021)
Article
Cell Biology
Pierre Balmer, William V. J. Hariton, Beyza S. Sayar, Vidhya Jagannathan, Arnaud Galichet, Tosso Leeb, Petra Roosje, Eliane J. Muller
Summary: This study investigates the functional role of SUV39H2 in maintaining the pool of stem and progenitor cells by restricting gene expression through inhibitory H3K9me3 marks on gene promoters. Loss of SUV39H2 function leads to premature exit of progenitor cells from the cell cycle due to enhanced Wnt activity, compromising epidermal differentiation and genome stability. SUV39H2 is identified as a major gatekeeper in regulating progenitor fate conversion through H3K9me3 rate-limiting road blocks in basal layer keratinocytes.
JOURNAL OF CELL BIOLOGY
(2021)