Journal
JOURNAL OF BIOTECHNOLOGY
Volume 145, Issue 1, Pages 79-83Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.10.011
Keywords
Cell surface display; Yeast; Biotin; Streptavidin; Biotin ligase
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Funding
- Ministry of Education, Science, Sports and Culture
- Grant-in-Aid for Scientific Research [13]
- MEXT, Japan
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Here we expand the yeast cell surface display system to display non-natural, functional molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli(BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through co-expression of BAP-fused Flo428 with BirA, biotinylated BAP could be displayed on the yeast cell surface. Subsequent addition of streptavidin-FITC resulted in the display of streptavidin-FITC, and, the display of biotin-FITC was successful using streptavidin as a linker. Our strategy provides a powerful tool for displaying functional molecules on yeast cell surfaces. (c) 2009 Elsevier B.V. All rights reserved.
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