Review
Biochemistry & Molecular Biology
Jie Liu, Jian-Zhong Xu, Bingbing Wang, Zhi-Ming Rao, Wei-Guo Zhang
Summary: L-valine is an essential branched-chain amino acid that has a wide range of applications in various industries. Traditional metabolic engineering is no longer sufficient to meet the demand for efficient biosynthesis of L-valine, and systematic metabolic engineering has become the new trend.
Article
Biotechnology & Applied Microbiology
Melanie Mindt, Lenny Ferrer, Dirk Bosch, Katarina Cankar, Volker F. Wendisch
Summary: Indole has gained increasing interest in the flavor and fragrance industry due to its distinct floral odor. However, the current production methods are non-sustainable and unprofitable. To offer an alternative approach, biosynthesis of indole has been studied, including glucose-based production using genetically-engineered strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Qinglan Lv, Mengkai Hu, Lingzhi Tian, Fei Liu, Qing Wang, Meijuan Xu, Zhiming Rao
Summary: The study successfully obtained a high-yielding L-glutamine-producing strain through ARTP mutagenesis and high-throughput screening, followed by using a series of rational metabolic strategies to further increase production. A two-stage pH control strategy was proposed to optimize the inconsistent effects of pH on cell growth and L-glutamine production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Taehee Han, Sang Yup Lee
Summary: The biosynthesis of Valerolactam (VL) was achieved by metabolically engineered Corynebacterium glutamicum, which overproduces L-lysine. This provides a green and sustainable method for the production of precursor chemicals for nylon-5 and nylon 6,5.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Lynn S. Schwardmann, Tong Wu, Aron K. Dransfeld, Steffen N. Lindner, Volker F. Wendisch
Summary: Researchers introduced the formamide/formamidase system from Helicobacter pylori into producer strains to achieve efficient formamide-based production of nitrogenous compounds. Stable isotope labeling confirmed the utilization of nitrogen from formamide and the exploitable ammonium leakage during formamidase-based access. Overexpression of formate dehydrogenase improved the efficient utilization of formamide as sole nitrogen source.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Zemin Li, Qiuting Wang, Hui Liu, Yating Wang, Zhaoyi Zheng, Yang Zhang, Tianwei Tan
Summary: In this study, the GlcNAc synthesis pathway was constructed in Corynebacterium glutamicum, resulting in increased yield and titer of GlcNAc. Optimal fermentation conditions and feeding strategy further enhanced the production, showing potential for industrial production of GlcNAc.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Eldin Kurpejovic, Volker F. Wendisch, Berna Sariyar Akbulut
Summary: The increase in elderly patients with Parkinson's disease is driving market growth for l-DOPA, with biotechnological approaches in Corynebacterium glutamicum cells showing potential for economically feasible production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shunsuke Ogata, Takashi Hirasawa
Summary: The study found that copper addition can induce glutamic acid production in Corynebacterium glutamicum, which requires OdhI, biotin limitation, penicillin addition, and a gain-of-function mutation in the NCgl1221 mechanosensitive channel. Disruption of the csoR gene can enhance copper-induced glutamic acid production in the NCgl1221 gain-of-function mutant strain, suggesting that unidentified CsoR-regulated genes may contribute to this process.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Cong Chen, Zhenzhen Cui, Juntao Zhao, Shuting Li, Xiaoting Ren, Tao Chen, Zhiwen Wang
Summary: The study investigated the use of Corynebacterium glutamicum as a chassis for diacetyl production by linking the diacetyl metabolic pathway and the respiratory chain to achieve redox balance. Through genetic modifications, a significant increase in diacetyl production was achieved with improved NADH/NAD+ ratio. The study showed that modifying the respiratory chain is an effective strategy to reduce intracellular cofactor imbalance and improve the production of diacetyl as well as other bio-based products in C. glutamicum.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lingling Shangguan, Huiyan Zhang, Zixiong Liu, Feiran An, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen
Summary: This study obtained a mutant strain, P-45, with improved glutamic acid production capacity and genetic stability through strain mutagenesis and screening. The glutamic acid concentration of P-45 under fed-batch fermentation conditions in 20 L tanks was increased by 81.5% compared to the starting strain.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Jaehyun Park, Dong Hun Kang, Han Min Woo
Summary: Engineering Corynebacterium glutamicum enabled the production and secretion of hydrophobic squalene without the need for in situ extractions, resulting in increased total squalene production. By promoting extraction and formulating bio-squalene nanoemulsions, creaming effects were analyzed for cosmetic applications. Combining metabolic pathway engineering and facilitated secretion in microbial cells shows promise for sustainable production of hydrophobic chemicals.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Agricultural Engineering
Dan Huang, Xin Wang, Wei-Bing Liu, Bang-Ce Ye
Summary: This study successfully remodeled the metabolic pathway of Corynebacterium glutamicum and achieved de novo synthesis of dencichine, offering a promising approach for natural product production through microbial fermentation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Bingnan Liu, Xinyu Sun, Yue Liu, Mengmeng Yang, Liang Wang, Ying Li, Jihui Wang
Summary: This study successfully increased the supply of reducing power NADPH and improved the production of L-methionine through genetic modification and heterologous expression. The engineered strain YM6 with high NADPH supply and high L-methionine production was constructed, and metabolic flux analysis confirmed the increase in its synthesis.
Article
Biochemistry & Molecular Biology
Soong-bin Kang, Jong-il Choi
Summary: The introduction of the response regulator DR1558 successfully enhanced the production of cadaverine in C. glutamicum under acidic conditions, indicating its impact on gene expression and stress defense mechanisms.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Arthur Burgardt, Carina Prell, Volker F. Wendisch
Summary: The study focuses on utilizing industrial sidestreams, such as wheat concentrate, to produce functionalized monomers for eco-friendly plastics. Metabolically engineered Corynebacterium glutamicum strains were used to efficiently convert WSC into l-lysine-derived bifunctional monomers, reducing environmental impact. Heterologous gene expression from Xanthomonas campestris and E. coli allowed for enhanced production of l-lysine and enabled production of cadaverine and 5-aminovalerate from WSCH.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Thermodynamics
Helena Dolezalova-Weissmannova, Stanislav Maly, Martin Brtnicky, Jiri Holatko, Michael Scott Demyan, Christian Siewert, David Tokarski, Eliska Kamenikova, Jiri Kucerik
Summary: Thermogravimetry (TG) is a simple method for rapid analysis of soil properties, but its link with microbiological soil properties has not been systematically tested. This study aimed to search and validate the relationships between thermal mass losses (TML) and various soil properties. Results showed significant correlations between TML and basic soil properties, and equations developed from these correlations were successfully validated for prediction, mainly for arable soils.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2023)
Article
Plant Sciences
Jiri Holatko, Tereza Hammerschmiedt, Antonin Kintl, Adnan Mustafa, Muhammad Naveed, Tivadar Baltazar, Oldrich Latal, Petr Skarpa, Pavel Ryant, Martin Brtnicky
Summary: Improving manure quality through co-composting with biochar and sulphur enhances soil fertility and crop growth. Sulphur-enriched manure types show significant effects on soil enzyme activities and respiration.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Misha Iqbal, Muhammad Naveed, Muhammad Sanaullah, Martin Brtnicky, M. Iftikhar Hussain, Jiri Kucerik, Jiri Holatko, Adnan Mustafa
Summary: This study aims to investigate the influence of microorganisms such as Enterobacter sp., Bacillus sp., and Piriformospora indica on canola growth, and it was found that they can significantly improve canola biomass, root system, and yield.
JOURNAL OF SOILS AND SEDIMENTS
(2023)
Article
Environmental Sciences
Maja Radziemska, Aurelia Blazejczyk, Mariusz Z. Gusiatin, Agnieszka Cydzik-Kwiatkowska, Grzegorz Majewski, Tereza Hammerschmiedt, Wioletta Rogula-Kozlowska, Malgorzata Majder-Lopatka, Jiri Kucerik, Ayla Bilgin, Jiri Holatko, Antonin Kintl, Martin Brtnicky
Summary: The addition of natural zeolite to trace-element-contaminated soil can increase plant biomass and soil pH, and significantly decrease the total content of copper, lead, cadmium, and zinc in the soil, as well as the bioavailable and leachable content of these elements. Moreover, the presence of zeolite can also influence the bacterial diversity in the soil, favoring the growth of certain bacterial species with ecological importance.
LAND DEGRADATION & DEVELOPMENT
(2023)
Article
Chemistry, Physical
Jiri Holatko, Martin Brtnicky, Adnan Mustafa, Antonin Kintl, Petr Skarpa, Pavel Ryant, Tivadar Baltazar, Ondrej Malicek, Oldrich Latal, Tereza Hammerschmiedt
Summary: Digestate with amendments improves soil microbiological properties and provides plants with available nutrients. Addition of biochar and sulphur in digestate can significantly enhance the effects on soil health and plant biomass. Short-term and long-term fertilization effects were observed, with increased total nitrogen and carbon, as well as sulphur and arylsulfatase activity. However, no significant differences were observed in aboveground plant biomass.
Article
Energy & Fuels
Maja Radziemska, Mariusz Zygmunt Gusiatin, Zbigniew Mazur, Algirdas Radzevicius, Agnieszka Bes, Raimondas Sadzevicius, Jiri Holatko, Midona Dapkiene, Inga Adamonyte, Martin Brtnicky
Summary: The presence of potentially toxic elements (PTEs) in soils can be harmful to the natural balance and increase the risk of PTE incorporation into the food chain. Utilizing composite biochar and municipal sewage sludge compost (MSSC/C) can effectively manage waste and support phytostabilization processes. The study confirmed that the addition of MSSC/C to PTE-contaminated soil increased plant biomass, improved soil pH, and reduced PTE contents, particularly Cd, Cr, and Ni.
Article
Multidisciplinary Sciences
Jiri Holatko, Tereza Hammerschmiedt, Adnan Mustafa, Antonin Kintl, Petr Skarpa, Pavel Ryant, Tivadar Baltazar, Ondrej Malicek, Oldrich Latal, Martin Brtnicky
Summary: This study aimed to evaluate the differences in the effect of unenriched manure and manures co-matured with biochar, elemental sulfur or both amendments. The results showed that co-matured manure with biochar had the highest rate of maturation and significantly increased dry aboveground biomass weight in the half-time of the experiment. However, this effect vanished after 24 weeks. On the other hand, co-matured manure with biochar and elemental sulfur led to short-term carbon sequestration due to presumed retardation of microbial-mediated transformation of nutrients, increasing the recalcitrancy of soil organic matter and slowing down soil nutrient transformation.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Tereza Hammerschmiedt, Jiri Holatko, Radim Zelinka, Antonin Kintl, Petr Skarpa, Zuzana Bytesnikova, Lukas Richtera, Adnan Mustafa, Ondrej Malicek, Martin Brtnicky
Summary: The impact of graphene oxide on soil properties is mixed, with both negative and positive effects. There are few studies on its single amendment to soil or in combination with nanosized sulfur. An eight-week pot experiment showed that graphene oxide had a positive effect on soil respiration, but its combination with high nano-sulfur had a negative effect on some types of respiration. Single application of graphene oxide enhanced arylsulfatase activity.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Environmental Sciences
Adnan Mustafa, Usman Zulfiqar, Muhammad Zahid Mumtaz, Maja Radziemska, Fasih Ullah Haider, Jiri Holatko, Tereza Hammershmiedt, Muhammad Naveed, Hassan Ali, Antonin Kintl, Qudsia Saeed, Jiri Kucerik, Martin Brtnicky
Summary: Scientists are interested in nickel (Ni) due to its essentiality and toxicity on terrestrial biota. It has been found that plants cannot complete their life cycle without an adequate supply of Ni. The safe limit of Ni for plants is 1.5 μg/g, and it plays crucial roles in various physiological functions.
Article
Agronomy
Jiri Holatko, Tereza Hammerschmiedt, Jiri Kucerik, Antonin Kintl, Tivadar Baltazar, Ondrej Malicek, Oldrich Latal, Martin Brtnicky
Summary: This research aimed to evaluate the effect of injecting digestate deeper into the soil profile of permanent grassland on soil and plant quality indicators. The results showed that the injector application of digestate improved long-term carbon sequestration, respiration, nitrate content, and urease activity. It also improved grass biomass yield and nutritional value, making it a viable agriculture management practice for digestate-based fertilisation of permanent grassland.
EUROPEAN JOURNAL OF AGRONOMY
(2023)
Article
Agronomy
Jakub Elbl, Antonin Kintl, Martin Brtnicky, Petr Sirucek, Jiri Mezera, Vladimir Smutny, Jan Vopravil, Jiri Holatko, Igor Hunady, Vojtech Lukas
Summary: This research investigates the impact of plot size changes on crop yield. The findings indicate that dividing plots into smaller parts increases the heterogeneity of crop yields and leads to an uneven distribution of yields. When dividing plots, higher yielding portions may accumulate in specific plots at the expense of others.
PLANT SOIL AND ENVIRONMENT
(2023)
Article
Environmental Sciences
Maja Radziemska, Mariusz Z. Gusiatin, Agnieszka Cydzik-Kwiatkowska, Aurelia Blazejczyk, Jiri Holatko, Martin Brtnicky
Summary: This article investigates the effect of a combined amendment, biochar+compost (BC), on the immobilization of Cd, Cu, Ni, Pb, and Zn in soil under freezing and thawing conditions (FTC). The study analyzes the speciation of these elements using sequential extraction and assesses the variability of the soil microbiome using 16S rRNA gene sequencing. The results show that BC promotes the biomass yield of L. perenne, but FTC decreases it. BC increases the content of heavy metals in plant roots and improves soil pH. BC has the greatest impact on increasing Cu stability by redistributing it from certain fractions to others. FTC affects the stability of heavy metals in the soil, especially for Pb and Zn. The core genera in the soil samples include Sphingomonas sp. and Mycobacterium sp., and FTC favors the growth of Bacteroidetes and Proteobacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agriculture, Multidisciplinary
Martin Brtnicky, Adnan Mustafa, Tereza Hammerschmiedt, Antonin Kintl, Lukas Trakal, Luke Beesley, Pavel Ryant, Carol Omara-Ojungu, Tivadar Baltazar, Jiri Holatko
Summary: This study investigates the effects of biochar application on soil microbial and plant parameters in agricultural soils. The results show that the addition of organic fertilizer with biochar significantly increases soil enzyme activity and plant biomass, while the activation of biochar reduces nutrient leaching from mineral fertilizers. Therefore, biochar has the potential to improve soil fertility and microbial activity in agriculture.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Agronomy
Antonin Kintl, Igor Hunady, Tomas Vitez, Martin Brtnicky, Julie Sobotkova, Tereza Hammerschmiedt, Monika Vitezova, Jiri Holatko, Vladimir Smutny, Jakub Elbl
Summary: The study found that silages made from mixed cultures of legumes intercropped with maize are almost of the same or even better quality in terms of dry matter yield compared with pure maize silage. The addition of different legumes in mixed silages significantly affected the content of various nutrients, with some legumes increasing certain components such as lipids and others decreasing components like neutral detergent fiber and starch. Furthermore, the production of biogas from these mixed substrates was directly proportional to the content of certain nutrients, showing potential for sustainable biogas production.
Article
Biotechnology & Applied Microbiology
Antonin Kintl, Igor Hunady, Jiri Holatko, Tomas Vitez, Tereza Hammerschmiedt, Martin Brtnicky, Vladena Ondriskova, Jakub Elbl
Summary: With the increasing number of biogas plants, the demand for suitable alternative plant substrates is also growing. Maize silage is currently the dominant substrate, but the intensive cultivation of maize as an energy crop poses environmental risks. This study presents the experimental methane production results from alternative substrates such as fodder mallow, white sweet clover, and their mixture.
FERMENTATION-BASEL
(2022)
