Article
Microbiology
Xiaoyan Chi, Yanhua Wang, Jing Miao, Wei Wang, Yanyang Sun, Zhifen Yu, Zhibin Feng, Shiwei Cheng, Lijuan Chen, Yihe Ge
Summary: The study showed that the transcriptional regulator gene eppR plays a crucial role in the production of phenazine-1-carboxylic acid in Pseudomonas chlororaphis G05, but has no effect on pyrrolnitrin biosynthesis.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Malik Jan, Sheng-Jie Yue, Ru-Xiang Deng, Yan-Fang Nie, Hong-Yan Zhang, Xiang-Rui Hao, Wei Wang, Hong-Bo Hu, Xue-Hong Zhang
Summary: Filamentous fungi are considered robust microorganisms for the biotransformation of natural products in green chemistry. In this study, a new fungus strain Aspergillus sclerotiorum was isolated, which efficiently converts phenazine-1-carboxylic acid (PCA) into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). An effective whole cells biotransformation system using optimal conditions and carbon sources was designed. The results showed promising potential for using filamentous fungi in large-scale biocatalysis of phenazine and efficient production of 3-OH-PCA.
FERMENTATION-BASEL
(2023)
Article
Microbiology
Youzhou Liu, Yaqiu Zhou, Junqing Qiao, Wenjie Yu, Xiayan Pan, Tingting Zhang, Yongfeng Liu, Shi-En Lu
Summary: The study found that phenazine-1-carboxylic acid (PCA) is the main antimicrobial compound produced by bacterial strain YL-1 in liquid medium, enhancing the inhibitory effects against Acidovorax citrulli by increasing the accumulation of reactive oxygen species and the activity of catalase.
Article
Biochemistry & Molecular Biology
Shouting Wu, Xi Liang, Fang Luo, Hua Liu, Lingyi Shen, Xianjiong Yang, Yali Huang, Hong Xu, Ning Wu, Qilong Zhang, Carl Redshaw
Summary: A phenazine-1-carboxylic acid intermediate was synthesized and used to obtain 10 new hydrazone compounds, one of which showed good cytotoxic activity in vitro cell experiments.
Article
Microbiology
Vijay K. K. Singh, Marianna Almpani, Kelsey M. M. Wheeler, Laurence G. G. Rahme
Summary: Pseudomonas aeruginosa is a common multidrug-resistant bacterial pathogen that poses a serious threat to critically ill and immunocompromised patients. Intestinal colonization by this pathogen is associated with increased mortality rates. In this study, the role of the three major communication systems in P. aeruginosa was investigated, and it was found that the MvfR system plays a key role in intestinal inflammation while the other systems have relatively minor effects. This study reveals the interrelationships of the quorum sensing systems during infection and provides important insights for developing effective antivirulence strategies.
Article
Chemistry, Applied
Yongtong Xiong, Xiang Zhu, Jinyu Hu, Yunping Wang, Xiaoying Du, Junkai Li, Qinglai Wu
Summary: Introducing amino acids into the phenazine ring can enhance the phloem mobility of phenazine carboxylic acid, compared to conjugating amino acids with the carboxyl group. Particularly, the compound 7d substituted with L-isoleucine showed a significantly higher phloem transport concentration than previous research.
NATURAL PRODUCT RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Kai Wang, Xia Li, Chunxi Yang, Shihao Song, Chaoyu Cui, Xiaofan Zhou, Yinyue Deng
Summary: The study demonstrated a new downstream regulator, Bcal3178, which controls biofilm formation and protease production in Burkholderia cenocepacia through modulation by AHL and BDSF QS systems. Bcal3178 regulates target gene expression by directly binding to promoter DNA regions, and it is directly controlled by the AHL system regulator CepR. These results unveil a novel molecular mechanism by which QS systems regulate target gene expression and biological functions in B. cenocepacia.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Seenivasan Boopathi, Rajesh Vashisth, Ashok Kumar Mohanty, Ai-Qun Jia, Natesan Sivakumar, Jesu Arockiaraj
Summary: Cell-to-cell communication is crucial for bacteria to produce virulence factors and colonize the host. The study reveals that Bacillus subtilis BR4 has potential activity in inhibiting biofilm formation and virulence factor production of Pseudomonas aeruginosa, making it a promising therapeutic agent to combat pathogenesis mediated by the quorum sensing system of P. aeruginosa.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yanjie Yu, Lijuan Chen, Mengyue Hao, Liyao Wang, Zhifen Yu, Yanhua Wang, Xiaoyan Chi, Zhibin Feng, Shiwei Cheng, Yihe Ge
Summary: This study elucidated the regulatory role of the pip gene in phenazine-1-carboxylic acid and pyrrolnitrin production in P. chlororaphis G05. Loss of pip gene significantly increased pyrrolnitrin production while decreasing phenazine-1-carboxylic acid synthesis.
BIOLOGICAL CONTROL
(2021)
Article
Biotechnology & Applied Microbiology
Qian Zhu, Xuekun Bai, Qian Li, Mingliang Zhang, Gang Hu, Kaihua Pan, Hongfei Liu, Zhijian Ke, Qing Hong, Jiguo Qiu
Summary: This study identified and characterized a transcriptional regulator PcaR that represses the transcription of the pcaA1A2A3A4 cluster and the pcaR gene. The binding site of PcaR in the ORF5205-pcaA1 intergenic promoter region contains a TNGT/ANCNA box, which is important for binding. These findings enhance our understanding of the molecular mechanism of PCA degradation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Plant Sciences
Xu-Jun Li, Wei Zhang, Chi-Na Zhao, Qing-Lai Wu, Jun-Kai Li, Zhi-Hong Xu
Summary: Novel triazole derivatives based on PCA were synthesized, showing strong fungicidal activity, with compounds 8b, 8h, and 8i displaying broad spectrum activity and compounds 8b, 8c, and 8h showing better efficacy against rice blast than PCA. These compounds are potential fungicidal candidates for further studies.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2021)
Article
Microbiology
Kaiquan Liu, Zhenghua Li, Xiaoli Liang, Yanpeng Xu, Yufei Cao, Ruiming Wang, Piwu Li, Ling Li
Summary: Phenazine-1-carboxylic acid (PCA) is a biologically active substance that can prevent and control crop diseases. A Pseudomonas chlororaphis strain, Lzh-T5, was used as the starting strain and genetic modifications were made to enhance PCA production. Through various strategies, PCA production was increased to 10,653 mg/L, indicating the potential of P. chlororaphis Lzh-T5 as an efficient cell factory for biologically active substances.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yun-Ling Fang, Ying Cui, Lian Zhou, Chitti Thawai, Tatheer Alam Naqvi, Hong-Yan Zhang, Ya-Wen He
Summary: The H-NS family member protein MvaU in Pseudomonas aeruginosa regulates PCA biosynthesis by self-regulation, repressing the expression of the phz2 cluster and consequently reducing PCA production. MvaU binds directly to the promoter of the phz2 cluster, demonstrating a new regulatory pathway for PCA biosynthesis in PA1201.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Selene Garcia-Reyes, Miguel Cocotl-Yanez, Martin Paolo Soto-Aceves, Abigail Gonzalez-Valdez, Luis Servin-Gonzalez, Gloria Soberon-Chavez
Summary: This study characterized the PqsE-dependent production of PYO in Pseudomonas aeruginosa ATCC 9027 and found that PqsE promotes the RhlR-dependent production of PYO, although this production is not strictly dependent on PqsE. The interaction between PqsE and RhlR modifies the transcription of genes involved in PYO production, highlighting the variability of the quorum-sensing response among different P. aeruginosa clades and strains.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xia Li, Gerun Wang, Quan Guo, Binbin Cui, Mingfang Wang, Shihao Song, Liang Yang, Yinyue Deng
Summary: Outer membrane vesicle (OMV)-delivered Pseudomonas quinolone signal (PQS) interferes with quorum sensing (QS) in Burkholderia cenocepacia by regulating the expression of QS signals and reduces competitiveness of B. cenocepacia. The LysR-type regulator ShvR is involved in the regulation of QS signals cis-2-dodecenoic acid (BDSF) and N-acyl homoserine lactone (AHL) by directly binding to their promoters. Perception of PQS influences the regulatory activity of ShvR and ultimately reduces QS signal production and virulence in B. cenocepacia.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kang Li, Gitte Petersen, Lisa Barco, Kristian Hvidtfeldt, Liping Liu, Anders Dalsgaard
Article
Environmental Sciences
Kang Li, Liping Liu, Jia Zhan, Marie-Louise Scippo, Kristian Hvidtfeldt, Yuan Liu, Anders Dalsgaard
SCIENCE OF THE TOTAL ENVIRONMENT
(2017)
Article
Microbiology
Yang Liu, Zheng Wang, Muhammad Bilal, Hongbo Hu, Wei Wang, Xianqing Huang, Huasong Peng, Xuehong Zhang
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Yujie Liu, Huimin Shi, Zheng Wang, Xianqing Huang, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Fisheries
Kang Li, Liping Liu, Jia Zhan, Marie-Louise Scippo, Anders Dalsgaard
AQUACULTURE RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Huimin Shi, Xianqing Huang, Zheng Wang, Yejun Guan, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Lingyu Wu, Zheng Wang, Yejun Guan, Xianqing Huang, Huimin Shi, Yujie Liu, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Zheng Wang, Xianqing Huang, Malik Jan, Deyu Kong, Jingwen Pan, Xuehong Zhang
Summary: The study revealed that the biosynthesis of antimicrobial secondary metabolites in Pseudomonas protegens H78 is regulated by the RNA chaperone Hfq and the catabolite repression control (Crc) protein. Hfq has a more extensive and intensive regulatory capacity compared to Crc, positively controlling gene clusters involved in secondary metabolism, secretion systems, and compound degradation.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zheng Wang, Xianqing Huang, Malik Jan, Deyu Kong, Wei Wang, Xuehong Zhang
Summary: Lon mutation significantly improves the antimicrobial activity of Pseudomonas chlororaphis HT66 strain by degrading PhzI protein, thus regulating the biosynthesis of PCN. Lon forms a new autoregulatory feedback circuit to modulate its own degradation and is responsible for the negative regulation of PCN biosynthesis and phzABCDEFG transcription mediated by PhzI in HT66.
MOLECULAR MICROBIOLOGY
(2021)
Article
Environmental Sciences
Zheng Wang, Xianqing Huang, Chenxi Nie, Tao Xiang, Xuehong Zhang
Summary: In P. protegens H78, Lon protease negatively regulates Plt biosynthesis by decreasing the abundance and stability of GacA and PltR proteins.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Environmental Sciences
Kamala Gharti, Li Yan, Kang Li, Nattida Boonpeng, Liping Liu
Summary: In-pond raceway aquaculture (IPRA) has been shown to effectively reduce water eutrophication, improve pond environment, and enhance fish production and quality. This study compared the growth performances and muscle quality of grass carp raised in IPRA and traditional pond culture (TPC). While there were no significant differences in hepatosomatic index, visceral mass ratio, and correction factor between IPRA and TPC, grass carp raised in IPRA exhibited higher weight gain rate and crude lipid content in their flesh. However, the pH, water holding capacity, hardness, and chewiness of grass carp muscle were similar between the two culture systems. The concentration of 2-MIB in the muscle increased continuously in IPRA. Additionally, the bacterial community analysis showed higher abundance of Proteobacteria in TPC and higher richness of Planctomycetes in IPRA. Despite the higher stocking density, the off-flavor in IPRA-produced grass carp had less impact on the aesthetic quality of their flesh compared to TPC. Overall, the results suggest that IPRA can produce grass carp with better muscle quality.
Article
Biotechnology & Applied Microbiology
Shuqi Guo, Yining Wang, Bona Dai, Wei Wang, Hongbo Hu, Xianqing Huang, Xuehong Zhang
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Xuemei Shen, Zheng Wang, Xianqing Huang, Hongbo Hu, Wei Wang, Xuehong Zhang
Article
Biotechnology & Applied Microbiology
Qiang Zhao, Hong-Bo Hu, Wei Wang, Xian-Qing Huang, Xue-Hong Zhang
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2017)
Article
Microbiology
Xianqing Huang, Zheng Wang, Yujie Liu, Xuehong Zhang
GENOME ANNOUNCEMENTS
(2017)