Article
Multidisciplinary Sciences
Masahiro Tominaga, Kenta Nozaki, Daisuke Umeno, Jun Ishii, Akihiko Kondo
Summary: The authors have introduced a workflow for parallel screening, selection and evolution of yeast genetic switches, aiming to address the complexity in eukaryotic genetic switch design.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Nai-Xin Lin, Rui-Zhen He, Yan Xu, Xiao-Wei Yu
Summary: The robust P. pastoris mutant demonstrates higher expression levels of not only lipase but also other heterogeneous proteins under methanol induction conditions. The mutant shows lower intracellular ROS levels compared to the WT. RNA-seq analysis revealed upregulation of key pathways related to antioxidant, MAPK pathway, ergosterol synthesis pathway, transcription factors, and the peroxisome pathway in the robust mutant, contributing to its enhanced oxidative stress tolerance and high heterologous protein expression efficiency.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Carlos Eduardo de Oliveira Nascimento, Lorena Caixeta de Oliveira Simoes, Josiani de Cassia Pereira, Ronivaldo Rodrigues da Silva, Evandro Antonio de Lima, Gabriel Cimonetti de Almeida, Ana Lucia Barretto Penna, Mauricio Boscolo, Eleni Gomes, Roberto da Silva
Summary: This study produced Xylooligosaccharides (XOs) by extracting xylan from sugarcane bagasse and hydrolyzing it using an enzyme. The XOs stimulated the growth of beneficial intestinal bacteria and could be used as a supplement to promote the growth of probiotic strains.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Peng Qin, Xin-Yi Lu, Jian-He Xu, Hui-Lei Yu
Summary: This study established an excellent expression system in Pichia pastoris to increase the secretion of Baeyer-Villiger monooxygenase and developed a high throughput screening method for directed evolution. The mutant with the highest protein expression level was obtained and high protein yield was achieved through high-density fermentation. This work is of great importance for efficient secretion of other BVMOs in Pichia pastoris.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Mirelle Flores-Villegas, Corinna Rebnegger, Viktoria Kowarz, Roland Prielhofer, Diethard Mattanovich, Brigitte Gasser
Summary: The promoter of the high-affinity glucose transporter Gth1 (PGTH1) was improved through random and rational engineering, resulting in stronger induction in glucose-limitation and enabling methanol-free production processes. Single point mutations and key promoter segments were identified, leading to a library of novel promoter variants with significantly higher activity. The study also discovered nucleotide positions and corresponding TFBS that distinguish between glycerol- and glucose-mediated repression of the native promoter.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Immunology
Nicholas J. MacDonald, Kavita Singh, Karine Reiter, Nguyen Vu, Richard Shimp, Apostolos G. Gittis, Beth Chen, Martin Burkhardt, Baoshan Zhang, Zhixiong Wang, Raul Herrera, Mackenzie Moler, Duck-Yeon Lee, Sachy Orr-Gonzalez, Jessica Herrod, Lynn E. Lambert, Kelly M. Rausch, Olga Muratova, David S. Jones, Yimin Wu, Albert J. Jin, David N. Garboczi, Patrick E. Duffy, David L. Narum
Summary: The development of a malaria vaccine that can block transmission of the parasite from mosquitoes to humans is crucial for malaria elimination efforts. The use of Pfs25, a protein on the surface of the parasite, is being investigated as a transmission-blocking vaccine. Two recombinant forms of Pfs25, Pfs25H and Pfs25M, have been studied for their structural and immunological differences.
Article
Biotechnology & Applied Microbiology
Ting Miao, Abdul Basit, Junquan Liu, Fengzhen Zheng, Kashif Rahim, Huiqiang Lou, Wei Jiang
Summary: The study improved the production of Myceliophthora thermophila xylanase through signal peptide engineering, resulting in enhanced enzyme activity. Further analysis showed that Mtxyn11C was more effective than commercial xylanase in degrading xylan, and when combined with other xylanolytic enzymes, it improved the hydrolysis efficiency of xylan.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Zhenyang Liu, Sitao Wen, Guogan Wu, Huawei Wu
Summary: A new glycoside hydrolase family 10 (GH10) xylanase gene, ArXyn10c20, from anaerobic rumen microorganism Anaeromyces robustus, was successfully synthesized and expressed. The recombinant xylanase showed optimal pH and temperature of 5.5 and 40 degrees Celsius, respectively. It exhibited stability in a pH range of 5.0-9.0 and its function was enhanced by the addition of 1 mM Cu2+. The hydrolysis products of ArXyn10c20 on beechwood xylan were xylobiose, xylotriose, and xylotetraose. Additionally, when added to bread making, ArXyn10c20 significantly improved dough and bread quality.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Biology
Binyang Deng, Yu Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, Chao Zhai
Summary: In this study, highly glycosylated IsPETase enzyme was obtained by controlling the glycosylation modification of PETase in Pichia pastoris, improving the enzyme's specific activity and thermostability. The partially deglycosylated IsPETase still maintained high thermostability and could completely degrade PET flakes at 50 degrees C. This study provides a strategy to modulate the thermostable IsPETase through glycosylation engineering, promoting PET biodegradation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Fidan Erden-Karaoglan, Mert Karaoglan
Summary: This study investigated the applicability of heterologous yeast promoters in Pichia pastoris, and found inducible and constitutive regulation modes as well as the optimal carbon sources for these promoters. The study also determined the DNA region with the highest activity for all promoters through promoter deletion analysis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Carmela Bonaccorsi di Patti, Antimo Cutone, Zuzana Pakanova, Marek Nemcovic, Peter Barath, Giovanni Musci
Summary: Ceruloplasmin is crucial for iron metabolism in vertebrates, and the production of high-quality recombinant human ceruloplasmin represents a significant advancement. Further improvement in productivity and glycoengineering is necessary for potential therapeutic use in aceruloplasminemia patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Hewen Deng, Mengjiao Li, Qiuyang Zhang, Chencheng Gao, Zhanyun Song, Chunhua Chen, Zhuo Wang, Xin Feng
Summary: Bacillary White Diarrhea, caused by Salmonella pullorum, is a severe disease that leads to high mortality in chicks. Antibiotic resistance has made treatment challenging, so a Salmonella bacteriophage endolysin, LySP2, was explored as a potential treatment option in this study.
Article
Biochemistry & Molecular Biology
Tianpeng Chen, Shimeng Wang, Huanqing Niu, Guanjia Yang, Sinan Wang, Yuqi Wang, Chaowei Zhou, Bin Yu, Pengpeng Yang, Wenjun Sun, Dong Liu, Hanjie Ying, Yong Chen
Summary: A biofilm-based fermentation system was constructed in P. pastoris by combining the surface display of beta-galactosidase and biofilm formation. The results showed a catalytic conversion rate of 50.3% for GOS with a maximum enzyme activity of 5125 U/g. This study provides a basis for developing biofilm-based surface display methods in P. pastoris.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Patricia Gomez de Santos, Manh Dat Hoang, Jan Kiebist, Harald Kellner, Rene Ullrich, Katrin Scheibner, Martin Hofrichter, Christiane Liers, Miguel Alcalde
Summary: In this study, two unusual acidic peroxygenases were successfully produced at a large scale in yeasts, with expression levels boosted to 290mg/liter through signal peptide modification and mutagenesis. These highly secreted, active, and stable peroxygenases have a broad pH activity profile, making them promising tools for future engineering endeavors and direct industrial application.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lin Cui, Huoqing Huang, Honglian Zhang, Xiaolu Wang, Xing Qin, Tao Tu, Jie Zhang, Xiaoyun Su, Huimin Yu, Yingguo Bai, Huiying Luo, Bin Yao, Yuan Wang
Summary: A novel fusion protein expression strategy was proposed to increase the expression level of hen egg white lysozyme (HEWL). The results showed that the fusion protein expressed using this strategy had higher expression level and activity compared to HEWL alone.
Article
Food Science & Technology
Zixing Dong, Xiaoming Hao, Daman S. Pokhrel, Xiaoling Chen, Xiaoguang Liu, Nokuthula P. Mchunu, Kugenthiren Permaul, Suren Singh, Dandan Niu, Zhengxiang Wang
Article
Agricultural Engineering
Bibhuti Ranjan, Santhosh Pillai, Kugenthiren Permaul, Suren Singh
BIORESOURCE TECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Jinfeng Sun, Jie Wang, Kangming Tian, Zixing Dong, Xiaoguang Liu, Kugenthiren Permaul, Suren Singh, Bernard A. Prior, Zhengxiang Wang
BIOTECHNOLOGY LETTERS
(2018)
Article
Biotechnology & Applied Microbiology
Kerisha Raghunandan, Ashwani Kumar, Santhosh Kumar, Kugenthiren Permaul, Suren Singh
Article
Biotechnology & Applied Microbiology
Kerisha Raghunandan, Ashwani Kumar, Santhosh Kumar, Kugenthiren Permaul, Suren Singh
Article
Engineering, Environmental
Bibhuti Ranjan, Santhosh Pillai, Kugenthiren Permaul, Suren Singh
JOURNAL OF HAZARDOUS MATERIALS
(2019)
Article
Agricultural Engineering
Johnson Tungamirai Zininga, Adarsh Kumar Puri, Algasan Govender, Suren Singh, Kugen Permaul
BIORESOURCE TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Ruth Nyanduko Okongo, Adarsh Kumar Puri, Zhengxiang Wang, Suren Singh, Kugen Permaul
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Agricultural Engineering
Meng Zhang, Adarsh Kumar Puri, Zhengxiang Wang, Suren Singh, Kugen Permaul
BIORESOURCE TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Bibhuti Ranjan, Philip H. Choi, Santhosh Pillai, Kugenthiren Permaul, Liang Tong, Suren Singh
Summary: The cyanase from Thermomyces lanuginosus has the highest catalytic efficiency among these enzymes, and its crystal structure study provides insights into both catalytic mechanism and inhibition, with potential applications in biotechnological fields.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Peili Shen, Dandan Niu, Kugen Permaul, Kangming Tian, Suren Singh, Zhengxiang Wang
Summary: This study identified 1443 differently expressed genes in Bacillus licheniformiscultivated in media supplemented with ammonia, with 859 upregulated and 584 downregulated genes. Ammonia-inducible promoters were shown to mediate amyL expression, with P-plp yielding the highest enzyme expression level. B. licheniformis possesses glucose-independent ammonia-inducible promoters that can enhance enzyme yield in fermentations traditionally fed with ammonia.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sarisha Singh, Bruce Sithole, Prabashni Lekha, Kugenthiren Permaul, Roshini Govinden
Summary: This study demonstrates the concept of cyclic fed-batch fermentation for enhanced PHA productivity by Bacillus thuringiensis using a glucose-rich hydrolyzate as the sole carbon source. The cyclic strategy resulted in almost threefold increase in biomass concentration and fourfold increase in PHA concentration compared with batch fermentation. The approach showcases the potential of coupling fermentation strategies with hydrolyzate from lignocellulosic waste to achieve high cell density biomass and enhanced PHA productivity.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Materials Science, Paper & Wood
Sarisha Singh, Bruce Sithole, Prabashni Lekha, Kugenthiren Permaul, Roshini Govinden
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
(2020)
