Article
Biochemistry & Molecular Biology
Hongliang Gao, Wei Zhang, Jing Zhang, Yimin Huang, Jingyu Zhang, Jiangtao Tian, Yanning Niu, Chunjing Zou, Caifeng Jia, Zhongyi Chang, Xuexia Yang, Deming Jiang
Summary: Curdlan, a water-insoluble exopolysaccharide produced by Agrobacterium species under nitrogen starvation, was significantly impaired in AmdeA, AmetA, AmetH, and AmetZ mutants. The impaired curdlan production was associated with down-regulation of genes involved in the electron transfer chain (ETC) and accumulation of ADP and FAD in the AmetH and AmetZ mutants. Addition of dibasic sodium succinate and methionine restored curdlan production in the mutants, indicating the importance of the methionine biosynthesis pathway in energy ATP supply during curdlan biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Minjie Gao, Zhilei Liu, Zhongsheng Zhao, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Zhiyong Zheng, Xiaobei Zhan
Summary: This study explored the roles of exoR and exoX genes in curdlan biosynthesis in Agrobacterium sp. ATCC 31749. The results showed that the curdlan yields and glucose utilization increased in delta exoR and delta exoX strains compared with the wild-type strain during batch fermentation. However, the curdlan yields and gel strength decreased in exoR and exoX overexpression strains.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Zhilei Liu, Ying Xu, Zichao Wang, Li Zhu, Zhitao Li, Yun Jiang, Xiaobei Zhan, Minjie Gao
Summary: During curdlan production, the encapsulation of Agrobacterium sp. by secreted exopolysaccharide (EPS) inhibits substrate uptake and curdlan synthesis. This study found that the addition of endo-ss-1,3-glucanase (BGN) effectively alleviated the EPS encapsulation effect and improved glucose consumption and curdlan yield. The upregulation of related genes and enhanced metabolic activity contributed to the increased production of curdlan.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Minjie Gao, Guoshuai Yang, Feifei Li, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Xiaobei Zhan
Summary: This study involved the production of curdlan oligosaccharides by expressing endo-11-1,3-glucanase in Pichia pastoris and constructing a co-culture system. The optimized conditions in a 7-L bioreactor led to a maximum yield of 18.77 g/L of curdlan oligosaccharides with 3-10 degrees of polymerization, presenting a novel strategy for oligosaccharide production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Ng. Kunjarani Chanu, Madan Kumar Mandal, Akanksha Srivastava, Neha Chaurasia
Summary: The study investigated the effects of pyrethroid insecticide alpha-cypermethrin on cyanobacteria Anabaena sp. NC-K1, revealing upregulation of various proteins involved in stress responses, photosynthesis, translation, and DNA repair. This proteomic modulation allowed Anabaena sp. NC-K1 to maintain cellular metabolism and homeostasis against pesticide stress.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Plant Sciences
Zheng-Jun Guan, Min Zheng, Zhi-Xi Tang, Wei Wei, C. Neal Stewart Jr
Summary: In this study, differential expression of total protein in leaves of two transgenic oilseed rape lines and their non-transgenic parent plant was analyzed. Fourteen differential protein spots were identified, including eleven upregulated and three downregulated proteins, involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. These changes in protein spots may be attributed to the insertion of foreign transgenes in transgenic oilseed rape, but transgenic manipulation does not necessarily cause significant changes in the proteomes of oilseed rape.
Article
Multidisciplinary Sciences
Lorenzo Toma, Giulia Vignali, Elisa Maffioli, Stefano Tambuzzi, Roberta Giaccari, Monica Mattarozzi, Simona Nonnis, Marco Milioli, Lorenzo Franceschetti, Gianluca Paredi, Armando Negri, Benedetta Riccardi, Cristina Cattaneo, Maria Careri, Gabriella Tedeschi, Stefano Bruno
Summary: Mass spectrometry (MS)-based proteomics has gained the attention of forensic pathologists. This study developed a shotgun bottom-up proteomic approach using rapid protein extraction and nano-liquid chromatography/high-resolution mass spectrometry on human skin. It identified potential biomarkers for bruise characterization and dating. The study successfully identified proteins specific to ecchymotic tissues, including the known biomarker Glycophorin A, suggesting its potential as a MS-detectable biomarker. Further analysis is needed to validate the findings and explore the potential of MS-based techniques in forensic pathology.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Agata Piasecka, Andrea Baier
Summary: This study investigated the impact of three trophic modes on the growth parameters and biochemical composition of Chlorella species. The results showed that mixotrophic cultivation using an agro-industrial by-product stimulated the growth of all species, yielding high amounts of protein-rich biomass. The study also provided insights into the proteome of Chlorella cells cultured under molasses supplementation conditions.
Article
Biochemistry & Molecular Biology
Jaime Barros, Him K. Shrestha, Juan C. Serrani-Yarce, Nancy L. Engle, Paul E. Abraham, Timothy J. Tschaplinski, Robert L. Hettich, Richard A. Dixon
Summary: Ammonia-lyases play a key role in coordinating the allocation of carbon for lignin synthesis and nitrogen availability for plant growth.
Article
Environmental Sciences
Juan Wang, Lin Lin, Da-Zhi Wang
Summary: Quantitative proteomic analysis revealed that OA exposure caused liver damage, including dilation of rough endoplasmic reticulum and mitochondrial swelling in hepatocytes. Increased serum malondialdehyde content and decreased hepatic protein phosphatase activity were dose-dependently associated with OA exposure. Apoptotic cells in the liver showed a significant dose-response relationship.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Plant Sciences
Saroj Kumar Sah, Salah Jumaa, Jiaxu Li, K. Raja Reddy
Summary: This study investigates the proteomic response of rice leaves to UV-B stress and identifies several proteins involved in the adaptation of rice to UV-B stress. The findings provide valuable insights for the development of UV-B stress-resistant rice varieties in the future.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Aldo Almeida, Bruno Trevenzoli Favero, Lemeng Dong, Pablo D. Gardenas, Jorge Saenz-Mata, Henrik Lutken, Soren Bak
Summary: Cucurbitaceae species are used in traditional medicine globally, and cucurbitacins have shown potent anticancer activity. Hairy roots of Cucurbita pepo can be used for metabolic engineering of cucurbitacins. The formation of hairy roots leads to changes in cucurbitacin accumulation, and transcription factors play a significant role in this process.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Swetha Sudha Nagamalla, Malini Devi Alaparthi, Sunitha Mellacheruvu, Ravindar Gundeti, Jana Priya Sony Earrawandla, Someswar Rao Sagurthi
Summary: The study investigated the impact of drought stress on two cotton varieties, revealing that Bt cotton showed stronger drought tolerance compared to non-Bt cotton at the protein expression and physiological levels. This finding may aid in breeding new drought-tolerant and high-yielding cotton hybrid varieties.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Environmental
Jiayu Zhang, Xiaoyan Li, Huaxin Lei, Renxin Zhao, Wenhui Gan, Kaiyan Zhou, Bing Li
Summary: Biological treatment is an efficient and economical method to remove thiamphenicol (TAP) residues from the environment. The discovery of TAP-degrading bacteria and the decryption of its biodegradation mechanism is beneficial for enhancing the biological removal of TAP. Sphingomonas sp. CL5.1 was found to be capable of catabolizing TAP, degrading a significant amount of TAP in a short time period. Additionally, a novel metabolism pathway of TAP was constructed with potential enzymes predicted for TAP metabolism in strain CL5.1.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Maria Luisa Castrejon-Godinez, Efrain Tovar-Sanchez, Ma Laura Ortiz-Hernandez, Sergio Encarnacion-Guevara, Angel Gabriel Martinez-Batallar, Magdalena Hernandez-Ortiz, Enrique Sanchez-Salinas, Alexis Rodriguez, Patricia Mussali-Galante
Summary: Methyl parathion, a widely used pesticide, poses a significant threat to ecosystems and human health due to its high toxicity and persistence in the environment. Identifying microorganisms capable of degrading methyl parathion is crucial for bioremediation efforts at pesticide-polluted sites.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)