Journal
JOURNAL OF BIOTECHNOLOGY
Volume 135, Issue 1, Pages 123-126Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2008.03.005
Keywords
Oldenlandia offinis; in vitro cultures; cyclotides; kalata B1 production; morphogenesis; drug discovery
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Cyclotides are naturally occurring mini-proteins that have a diverse range of therapeutically useful biological activities. Although a choice of approaches is available for cyclotides synthesis; most studies have involved the use of peptides extracted from plants. in order to facilitate the screening for structure-activity studies or to exploit them in drug development, a convenient and reliable route for the biosynthesis of cyclotides is of vital importance. Callus, suspension cultures and hydroponic plants of Oldenlandia affinis were established and have been evaluated for effective cyclotides production processes. The specific accumulation of kalata B1 was affected by cell differentiation as well as agitation; highest accumulation of 2.7 mg g(-1) dry weight was detected in agitated hydroponic plant cultures resulting in a productivity of 1.4 mg kalata B1 l(-1) day(-1). (C) 2008 Elsevier B.V. All rights reserved.
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