Journal
JOURNAL OF BIOTECHNOLOGY
Volume 134, Issue 1-2, Pages 88-92Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2007.12.012
Keywords
Escherichia coli JM109; hirudin; high cell density culture
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A synthetic medium, TK-25, for high cell density cultivation (HCDC) of Escherichia coli K-12 was modified to support HCDC of strain JM109. By optimizing the culture conditions, the cell concentration of 65g/l in 14 h was obtained in the optimized medium, namely TK-10, with glucose-fed batch cultivation. When these conditions were further applied for HCDC of E coli JM109 harboring pUC-based recombinant plasmid which expresses a hirudin variant, HV-1-fused protein under the control of trp promoter, it grew to 24 g/l of dried cells expressed as an inclusion body as 15.9% of the total protein, corresponding to 1908 mg/l hirudin-fused protein. (C) 2008 Elsevier B.V. All rights reserved.
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