Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 117, Issue 6, Pages 730-736Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2013.11.013
Keywords
Biogas; Agricultural wastes; 16S rRNA genes; mcrA genes; Terminal restriction fragment length polymorphism
Funding
- Russian Federal Target Program Research and development on priority directions of scientific-technological complex of Russia [16.515.11.5043]
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In this work bacteria and methanogenic archaea utilizing agricultural wastes in a pilot-scale biogas reactor were examined using sequencing and terminal restriction fragment length polymorphism analysis. Based on the analyses of 16S rRNA genes, Clostridia represented the most diverse group in the digester. Of the Clostridia, unclassified Clostridiales and the members of the genera Anaerotruncus and Tissierella were detected at high abundances. The representatives of the bacterial phyla Bacteroidetes and Proteobacteria were also defined, but in minor proportions, and were assigned to non-dominant communities. Within the phylum Euryarchaeota, the members of the orders Methanosarcinales and Methanomicrobiales were found at high levels. Methanogenic archaea were analyzed using both 16S rRNA and mcrA genes. Actually good results were received using both approaches; however, the rRNA gene method missed the non-dominant order Methanobacteriales. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
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