Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 116, Issue 1, Pages 85-90Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2013.01.017
Keywords
Lactic acid; D-isomer; Ethanol; Saccharomyces cerevisiae; Household waste
Funding
- New Energy and Industrial Technology Development Organization (NEDO)
- Independent Administrative Legal Entity, Japan Society for the Promotion of Science (JSPS) [23658082]
- Grants-in-Aid for Scientific Research [23658082] Funding Source: KAKEN
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The lactic acid-assimilating yeast Saccharomyces cerevisiae NAM34-4C grew rapidly in minimal D-lactate medium (pH 3.5) at 35 degrees C, compared with minimal L-lactate medium. A laboratory strain, S. cerevisiae S288C, did not grow in either medium at pH 3.5. Strain NAM34-4C produced remarkably high levels of ethanol in YPDL medium at pH 3.5, but not at pH 5.5, when D-lactate was provided as the carbon source. Optimal cultivation conditions for ethanol production from is-lactate by strain NAM34-4C were as follows: shaking speed, 60 rpm; initial pH, 3.0; cultivation temperature, 35 degrees C; yeast extract, 5 g/L; peptone, 10 g/L; and D-lactate, 30 g/L. Under these conditions, strain NAM34-4C produced 2.7 g/L ethanol, which is 18% of the theoretical maximal yield (0.51 x initial D-lactate concentration). (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
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