Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 116, Issue 5, Pages 644-646Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2013.05.004
Keywords
Bleomycin resistant gene; DNA fragmentation; Overdose; Zeocin
Ask authors/readers for more resources
DNA-damaging agent Zeocin is frequently used for vector selection in multiple cell types. We found that vector extracted from the Escherichia coli cells overgrown in the presence of Zeocin was generally contaminated with genomic DNA fragments. High dosage or prolonged cultivation should thus be avoided to minimize the contamination. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
