Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 112, Issue 5, Pages 515-517Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2011.07.011
Keywords
In vitro selection; Ribosome display; Peptide library; Binding peptide; Peptide aptamer
Funding
- Grants-in-Aid for Scientific Research [22220009] Funding Source: KAKEN
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A newly developed ribosome display protocol was applied to the in vitro selection of polypeptide aptamers to small molecular weight chemicals, 6-[hydroxy(4-nitrobenzyl)phosphonyl]hexanoic acid and vitamin B12, chosen from a peptide library of random sequences. New peptide sequences binding to the targets were found after six rounds of this protocol. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
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