☆ 4.4 Article

Dual production of poly (3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2009)

Journal

JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Volume 107, Issue 4, Pages 409-411

Publisher

SOC BIOSCIENCE BIOENGINEERING JAPAN

DOI: 10.1016/j.jbiosc.2008.12.003

Keywords

Biotin; Corynebacterium glutamicum; Dual production; Glutamate; Poly(3-hydroxybutyrate)

Categories

Biotechnology & Applied Microbiology Food Science & Technology

Funding

Ministry of Education, Culture, Sports, Science and Technology of Japan [70216828]

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Abstract

We previously synthesized poly(3-hydroxybutyrate) [P(3HB)] in recombinant Corynebacterium glutamicum, a prominent producer of amino acids. In this study, a two-step cultivation was established for the dual production of glutamate and P(3HB) due to the differences in the optimal concentration of biotin. Glutamate was extracellularly produced first under the biotin-limited condition of 0.3 mu g/L. Production was then shifted to P(3HB) by addition of biotin to a total concentration of 9 mu g/L. The final products obtained were 18 g/L glutamate and 36 wt% of P(3HB). (c) 2008, The Society for Biotechnology, Japan. All rights reserved.

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