Article
Biochemistry & Molecular Biology
Dilek Yonar, Mete Severcan, Rafig Gurbanov, Abdulsamet Sandal, Ulku Yilmaz, Salih Emri, Feride Severcan
Summary: This study successfully developed a new approach for the characterization and diagnosis of malignant pleural mesothelioma (MPM) among lung diseases using Fourier transform infrared spectroscopy (FTIR) coupled with multivariate analysis. The results showed that the MPM group could be successfully differentiated from healthy, benign exudative effusion, and lung cancer groups based on the changes in lipid biosynthesis and nucleic acids levels in the malignant serum samples.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Health Care Sciences & Services
Francis L. Martin, Camilo L. M. Morais, Andrew W. Dickinson, Tarek Saba, Thomas Bongers, Maneesh N. Singh, Danielle Bury
Summary: Saliva can be used as a liquid biopsy for screening lung cancer and prostate cancer. By analyzing the bioconstituents in saliva samples using infrared spectroscopy and principal component analysis, a highly accurate model can be built for the detection of these cancers in primary care settings.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Engineering, Chemical
Atiqah Ab Aziz, Veenesh Selvaratnam, Yasmin Fadzlin Binti Ahmad Fikri, Muhamad Shirwan Abdullah Sani, Tunku Kamarul
Summary: The present study aimed to develop a rapid non-destructive detection method for early diagnosis of osteoarthritis (OA) through evaluating infrared spectroscopy combined chemometrics. The results showed that ATR-FTIR spectroscopy combined with chemometrics analysis could detect spectral biomarkers for discriminating OA patients, providing a rapid and cost-effective blood test for early diagnosis of human OA.
Article
Multidisciplinary Sciences
Helina Heino, Lassi Rieppo, Tuija Mannisto, Mikko J. Sillanpaa, Vesa Mantynen, Simo Saarakkala
Summary: ATR-FTIR spectroscopy coupled with machine learning-based PLS-DA was used to study the detection of SARS-CoV-2 from nasopharyngeal swab samples. The results indicate that the ATR-FTIR spectrum contains specific information for SARS-CoV-2 infection, but the diagnostic performance is moderate.
SCIENTIFIC REPORTS
(2022)
Article
Spectroscopy
Cristina Cano-Trujillo, Carmen Garcia-Ruiz, Fernando E. Ortega-Ojeda, Gemma Montalvo
Summary: Blood is analyzed with ATR FTIR spectroscopy combined with OPLS-DA models to differentiate it from other substances at crime scenes. The study successfully distinguishes blood from environmental interfering substances regardless of the substrate it is on and identifies bloodstains according to the substrate they were deposited on. These findings provide a proof-of-concept for distinguishing bloodstains from interfering substances on common crime scene substrates.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Thermodynamics
R. Skvorcnskiene, I. Kiminaite, L. Vorotinskiene, A. Jancauskas, R. Paulauskas
Summary: Today's society faces the dual challenge of resource depletion and waste accumulation. There is a growing demand to replace conventional plastics with bioplastics in the market, but the sustainability of bioplastics compared to conventional plastics remains a subject of debate due to limited research. This study investigates the degradation characteristics of bioplastics in soil (ATR-FTIR), their thermal stability under pyrolysis, and their suitability for waste-to-energy conversion using thermal analysis (TG) and evolved gas analysis (FTIR). The biodegradation experiments reveal that only corn starch-based bioplastics can completely and rapidly degrade in soil, while other bio-based and petroleum-based plastics only undergo color change and softening. This indicates that bioplastics undergo thermochemical alteration and closely resemble conventional plastics, necessitating proper recycling or conversion into energy to avoid becoming a source of microplastics. Thermogravimetric analysis coupled with evolved gas analysis (TGA-FTIR) shows that the gas yield from bioplastics ranges from 80-99%, with degradation onset occurring at temperatures ranging from 203.0-272.5 degrees C in a nitrogen environment, or 10-20 degrees C lower in a steam-enriched environment. The main volatile products of bioplastics pyrolysis are carboxylic compounds, alkanes, alkenes, aromatic hydrocarbons, amines, CO, and CO2, depending significantly on the chemical composition of the plastic.
Article
Chemistry, Analytical
David Mabwa, Ketankumar Gajjar, David Furniss, Roberta Schiemer, Richard Crane, Christopher Fallaize, Pierre L. Martin-Hirsch, Francis L. Martin, Theordore Kypraios, Angela B. Seddon, Sendy Phang
Summary: This study demonstrates the discrimination of endometrial cancer from benign controls using mid-infrared spectroscopy of dried plasma or serum samples. Different spectral data ranges have an impact on discrimination results, and good classification performance can still be achieved even when a section of the bio-fingerprint region is removed.
Article
Environmental Sciences
Agata Egea-Corbacho, Ana Pilar Martin-Garcia, Ana A. Franco, Gemma Albendin, Juana M. Arellano, Rocio Rodriguez-Barroso, M. Dolores Coello, Dolores Coello, Jose F. Cabello, Iria Iglesias Prado, Erik-jan Malta
Summary: This study investigates the impact of microplastics (MP) on aquaculture systems, covering water intake, purification treatment, culture tanks, feed, and the organism. The study shows that purification systems can reduce the entry of MP from the environment, but new MP are observed in the sea bass tank, mainly due to added feed and materials used in aquaculture systems. Different types of MP are found in the sea bass samples. Consideration and reduction of other entry routes are necessary to prevent MP from reaching the human consumer in aquaculture facilities.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Abolfazl Dashti, Yannick Weesepoel, Judith Muller-Maatsch, Hadi Parastar, Farzad Kobarfard, Bahram Daraei, Hassan Yazdanpanah
Summary: The feasibility of portable Fourier transform infrared spectroscopy combined with multivariate classification techniques for meat classification and halal meat species certification was assessed in this study. The results showed that the combination of Fourier transform infrared spectroscopy and multivariate classification techniques can accurately and rapidly classify meat samples and certify halal meat species.
MICROCHEMICAL JOURNAL
(2022)
Article
Oncology
Alexandra Sala, James M. Cameron, Cerys A. Jenkins, Hugh Barr, Loren Christie, Justin J. A. Conn, Thomas R. Jeffry Evans, Dean A. Harris, David S. Palmer, Christopher Rinaldi, Ashton G. Theakstone, Matthew J. Baker
Summary: Pancreatic tumors are difficult to detect early, and current diagnostic tests are not specific. This study used infrared spectroscopy to distinguish pancreatic cancers from healthy and symptomatic controls, showing high detection rates. Simple, minimally invasive, and accurate approaches can aid in early detection of pancreatic cancer and improve patient prognosis and quality of life.
Article
Chemistry, Analytical
Ahmed M. Othman, Yasser M. Sabry, Diaa Khalil, Bassam Saadany, Tarik Bourouina
Summary: In this study, a disposable and low-cost micro-transmission cell was proposed for mid-infrared spectroscopy of liquid biological samples. The cell was constructed by dispersing plastic microspheres in the liquid sample and sandwiching them between two silicon windows, allowing for near-optimum optical interaction path length. The results showed that the performance of this cell was comparable to that of the standard attenuated total reflection (ATR) spectroscopy method.
ANALYTICAL METHODS
(2023)
Article
Medical Laboratory Technology
Aniello Primiano, Silvia Persichilli, Flavio Di Giacinto, Gabriele Ciasca, Silvia Baroni, Pietro Manuel Ferraro, Marco De Spirito, Andrea Urbani, Jacopo Gervasoni
Summary: The study evaluated the combination of infrared spectroscopy with chemometric analysis as a screening method to identify patients requiring confirmation by HPLC-FL analysis for cystinuria diagnosis. The results showed that this approach was both sensitive and specific for detection of the disorder.
CLINICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
H. Lee, D. Lee, J. M. Seo
Summary: This study collected and analyzed paint from an actual site of ship collision, comparing paint traces of the damaged ship and the ship suspected to be responsible for the collision through various chemical analysis methods. The ship responsible for the collision could be identified by performing a comparative analysis of the extracted paint.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Rosario Javier-Astete, Jessenia Melo, Jorge Jimenez-Davalos, Gaston Zolla
Summary: This study validated a model for the classification of wood species and a universal model for the rapid determination of cellulose, hemicellulose, and lignin using FTIR spectroscopy coupled with chemometrics. The results showed that the full spectra can be used to differentiate different species and identify infrared peaks associated with cellulose, hemicellulose, and lignin. In addition, the full spectra helped build a three species universal model for quantifying the principal wood chemical components. Therefore, the study demonstrated that FTIR-ATR, together with chemometrics, is a reliable method for discriminating wood species and determining wood chemical composition.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Medicinal
Joao F. S. Rodrigues, Liziane C. M. da Silva, Leia Cardoso-Sousa, Douglas Carvalho Caixeta, Debora D. Luckemeyer, Alisson S. Henrique, Jaqueline P. Pontes, Lycia M. G. da Silva, Juliana S. S. Macedo, Pedro S. Carvalho Junior, Cristiane Silva e Silva, Mahiba M. R. S. Martins, Valerio Monteiro-Neto, Marcos A. G. Grisotto, Anita M. R. Fernandes, Juliano Ferreira, Joao B. Calixto, Robinson Sabino-Silva, Elizabeth S. Fernandes
Summary: The study investigated the potential use of flow cytometry and ATR-FTIR spectroscopy as measures to identify responders to leflunomide in rheumatoid arthritis patients. The results indicate that both flow cytometry and ATR-FTIR can potentially be employed as additional measures to monitor early treatment response to leflunomide in RA patients, with the ability to differentiate responders from non-responders three months after therapy initiation.
Article
Oncology
Alexandra K. Walker, Katalin Karaszi, Helen Valentine, Victoria Y. Strauss, Ananya Choudhury, Shaun McGill, Kaisheng Wen, Michael D. Brown, Vijay Ramani, Selina Bhattarai, Mark T. W. Teo, Lingjian Yang, Kevin A. Myers, Nayneeta Deshmukh, Helen Denley, Lisa Browning, Sharon B. Love, Gopa Iyer, Noel W. Clarke, Emma Hall, Robert Huddart, Nicholas D. James, Peter J. Hoskin, Catharine M. L. West, Anne E. Kiltie
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2019)
Article
Multidisciplinary Sciences
Rachel Eyre, Denis G. Alferez, Angelica Santiago-Gomez, Kath Spence, James C. McConnell, Claire Hart, Bruno M. Simoes, Diane Lefley, Claudia Tulotta, Joanna Storer, Austin Gurney, Noel Clarke, Mick Brown, Sacha J. Howell, Andrew H. Sims, Gillian Farnie, Penelope D. Ottewell, Robert B. Clarke
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Nga-Tsing Tang, Richard D. Snook, Mick D. Brown, Bryan A. Haines, Andrew Ridley, Peter Gardner, Joanna L. Denbigh
Article
Oncology
Nicholas D. James, Noel W. Clarke, Adrian Cook, Adnan Ali, Alex P. Hoyle, Gerhardt Attard, Christopher D. Brawley, Simon Chowdhury, William R. Cross, David P. Dearnaley, Johann S. de Bono, Carlos Diaz-Montana, Duncan Gilbert, Silke Gillessen, Clare Gilson, Rob J. Jones, Ruth E. Langley, Zafar I. Malik, David J. Matheson, Robin Millman, Chris C. Parker, Cheryl Pugh, Hannah Rush, J. Martin Russell, Dominik R. Berthold, Michelle L. Buckner, Malcolm D. Mason, Alastair W. S. Ritchie, Alison J. Birtle, Susannah J. Brock, Prantik Das, Dan Ford, Joanna Gale, Warren Grant, Emma K. Gray, Peter Hoskin, Mohammad M. Khan, Caroline Manetta, Neil J. McPhail, Joe M. O'Sullivan, Omi Parikh, Carla Perna, Carmel J. Pezaro, Andrew S. Protheroe, Angus J. Robinson, Sarah M. Rudman, Denise J. Sheehan, Narayanan N. Srihari, Isabel Syndikus, Jacob S. Tanguay, Carys W. Thomas, Salil Vengalil, John Wagstaff, James P. Wylie, Mahesh K. B. Parmar, Matthew R. Sydes
Summary: Abiraterone acetate plus prednisolone improves overall survival in prostate cancer patients receiving long-term hormone therapy, regardless of metastatic disease risk group.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Multidisciplinary Sciences
Ashwin Sachdeva, Claire A. Hart, Christopher D. Carey, Amy E. Vincent, Laura C. Greaves, Rakesh Heer, Pedro Oliveira, Michael D. Brown, Noel W. Clarke, Doug M. Turnbull
Summary: Advances in multiplex immunofluorescence and digital image analysis have allowed simultaneous assessment of protein defects in electron transport chain components. We developed an automated high-throughput mIF workflow for quantitative single-cell level assessment of formalin fixed paraffin embedded tissue. Using this method, we identified alterations in mitochondrial oxidative phosphorylation protein in prostate samples and observed differences in OXPHOS protein abundance.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Ashwin Sachdeva, Claire A. Hart, Kyungmin Kim, Thomas Tawadros, Pedro Oliveira, Jonathan Shanks, Mick Brown, Noel Clarke
Summary: This study investigated the influence of EphA2 signaling in prostate cancer (PCa) progression and long-term survival. The results showed that non-canonical EphA2 signaling, mediated by pEphA2(S897), plays a key role in transendothelial migration of PCa to bone marrow endothelium. High expression of EphA2 or pEphA2(S897) in a PTENlow background is associated with poor overall survival.
BRITISH JOURNAL OF CANCER
(2022)
Article
Spectroscopy
Erin Bruner, Geraldine Monjardez
Summary: Identifying the type of body fluid recovered from crime scenes is crucial before DNA typing. This study develops a method using biosynthesized SERS swabs for the collection and identification of nonhuman blood, providing a more accurate and non-destructive detection technique.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Nga Tsing Tang, Richard Robinson, Richard D. Snook, Mick Brown, Noel Clarke, Peter Gardner
Summary: Bladder cancer is a common cancer with non-obvious symptoms, making it difficult to detect early. Urine samples contain bladder cells that could potentially be used for early detection. Raman spectroscopy, combined with laser tweezers, has been used to characterize bladder cells in urine samples. The results show that invasive bladder cancer cells can be accurately identified with over 87% accuracy using principal component-canonical variates analysis (PC-CVA) and k-fold validation, indicating the potential for developing an early detection method.
Article
Oncology
Gerhardt Attard, Laura Murphy, Noel W. Clarke, Ashwin Sachdeva, Craig Jones, Alex Hoyle, William Cross, Robert J. Jones, Christopher C. Parker, Silke Gillessen, Adrian Cook, Chris Brawley, Clare Gilson, Hannah Rush, Hoda Abdel-Aty, Claire L. Amos, Claire Murphy, Simon Chowdhury, Zafar Malik, J. Martin Russell, Nazia Parkar, Cheryl Pugh, Carlos Diaz-Montana, Carmel Pezaro, Warren Grant, Helen Saxby, Ian Pedley, Joe M. O'Sullivan, Alison Birtle, Joanna Gale, Narayanan Srihari, Carys Thomas, Jacob Tanguay, John Wagstaff, Prantik Das, Emma Gray, Mymoona Alzouebi, Omi Parikh, Angus Robinson, Amir H. Montazeri, James Wylie, Anjali Zarkar, Richard Cathomas, Michael D. Brown, Yatin Jain, David P. Dearnaley, Malcolm D. Mason, Duncan Gilbert, Ruth E. Langley, Robin Millman, David Matheson, Matthew R. Sydes, Louise C. Brown, Mahesh K. B. Parmar, Nicholas D. James
Summary: This study analyzed two open-label, randomized, controlled phase 3 trials and evaluated the long-term outcomes and survival benefits of combining abiraterone and enzalutamide with androgen deprivation therapy in metastatic prostate cancer patients. The results showed that the combination of abiraterone and enzalutamide did not improve survival compared to androgen deprivation therapy alone, but the addition of abiraterone to androgen deprivation therapy had clinically significant improvements in survival.
Article
Medicine, Legal
Makenzie Kuehn, Kevin Bates, J. Tyler Davidson, Geraldine Monjardez
Summary: The detection of intact explosives in the field is a unique challenge, and handheld Raman instruments have become popular for their speed, reliability, and portability. However, there is limited research on the detection capabilities of these instruments for explosive compounds. This study evaluated the detection capabilities of two handheld Raman spectrometers and investigated interference from glass and plastic containers. The results showed reproducible spectra for all analytes across both instruments, with the Rigaku ResQ-CQL displaying better resolution.
FORENSIC SCIENCE INTERNATIONAL
(2023)
Article
Oncology
Nicholas D. James, Fiona C. Ingleby, Noel W. Clarke, Claire L. Amos, Gerhardt Attard, Christopher D. Brawley, Simon Chowdhury, William Cross, David P. Dearnaley, Duncan C. Gilbert, Silke Gillessen, Robert J. Jones, Ruth E. Langley, Archie Macnair, Zafar Malik, Malcolm D. Mason, David J. Matheson, Robin Millman, Chris C. Parker, Hannah L. Rush, J. Martin Russell, Carly Au, Alastair W. S. Ritchie, Ricardo Pereira Mestre, Imtiaz Ahmed, Alison J. Birtle, Susannah J. Brock, Prantik Das, Victoria A. Ford, Emma K. Gray, Robert J. Hughes, Caroline B. Manetta, Duncan B. McLaren, Ashok D. Nikapota, Joe M. O'Sullivan, Carla Perna, Clive Peedell, Andrew S. Protheroe, Santhanam Sundar, Jacob S. Tanguay, Shaun P. Tolan, John Wagstaff, Jan B. Wallace, James P. Wylie, Anjali Zarkar, Mahesh K. B. Parmar, Matthew R. Sydes
Summary: This study found that adding docetaxel did not significantly improve metastatic progression-free survival in non-metastatic prostate cancer patients receiving long-term treatment. However, it did improve failure-free survival and progression-free survival without increasing late toxicity. There was no strong evidence for overall survival benefit.
JNCI CANCER SPECTRUM
(2022)
Article
Chemistry, Analytical
Dougal Ferguson, Alex Henderson, Elizabeth F. McInnes, Rob Lind, Jan Wildenhain, Peter Gardner
Summary: This article reviews the potential of using infrared microscopy techniques, such as FTIR and QCL, for the detection and classification of cancerous tissues. It also suggests using the F1-Score as a quantitative metric to compare model performances. Furthermore, the article provides suggestions for data handling techniques and reporting standards for future studies.
Article
Chemistry, Analytical
Jiayi Tang, Alex Henderson, Peter Gardner
Summary: The use of infrared spectroscopy in histopathology shows promise for diagnosing various diseases. Comparing the performance of AdaBoost and Random Forests algorithms on non-uniform data sets, it was found that both algorithms demonstrated excellent classification accuracy on breast cancer tissue samples, with AdaBoost showing more robustness in handling imbalanced datasets. This study provides insights into the choice of machine learning approach for building models in histopathology.
Article
Urology & Nephrology
Kenneth Hiew, Claire A. Hart, Adnan Ali, Tony Elliott, Vijay Ramani, Vijay Sangar, Maurice Lau, Satish Maddineni, Mick Brown, Noel Clarke
EUROPEAN UROLOGY FOCUS
(2019)
Article
Chemistry, Analytical
James Doherty, Alan Raoof, Ansaf Hussain, Magda Wolna, Gianfelice Cinque, Michael Brown, Peter Gardner, Joanna Denbigh
