Journal
JOURNAL OF BIOPHOTONICS
Volume 7, Issue 8, Pages 589-596Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201200244
Keywords
fluorescence microscopy; multispectral and hyper spectral imaging; image analysis; spectral unmixing; tissue imaging; human skin
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Skin contains many autofluorescent components that can be studied using spectral imaging. We employed a spectral phasor method to analyse two photon excited auto-fluorescence and second harmonic generation images of in vivo human skin. This method allows segmentation of images based on spectral features. Various structures in the skin could be distinguished, including Stratum Corneum, epidermal cells and dermis. The spectral phasor analysis allowed investigation of their fluorescence composition and identification of signals from NADH, keratin, FAD, melanin, collagen and elastin. Interestingly, two populations of epidermal cells could be distinguished with different melanin content.
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