4.6 Article

Coupled external cavity photonic crystal enhanced fluorescence

Journal

JOURNAL OF BIOPHOTONICS
Volume 7, Issue 5, Pages 332-340

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201200173

Keywords

photonic crystal; fluorescence enhancement; external-cavity laser; nanostructured surface

Funding

  1. National Science Foundation [CBET11-32225]
  2. National Institutes of Health [R01 GM086382]

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We report a fundamentally new approach to enhance fluorescence in which surface adsorbed fluorophore-tagged biomolecules are excited on a photonic crystal surface that functions as a narrow bandwidth and tunable mirror of an external cavity laser. This scheme leads to approximate to 10x increase in the electromagnetic enhancement factor compared to ordinary photonic crystal enhanced fluorescence. In our experiments, the cavity automatically tunes its lasing wavelength to the resonance wavelength of the photonic crystal, ensuring optimal on-resonance coupling even in the presence of variable device parameters and variations in the density of surface-adsorbed capture molecules. We achieve approximate to 10(5)x improvement in the limit of detection of a fluorophore-tagged protein compared to its detection on an unpatterned glass substrate. The enhanced fluorescence signal and easy optical alignment make cavity-coupled photonic crystals a viable approach for further reducing detection limits of optically-excited light emitters that are used in biological assays. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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