Article
Optics
Jianan Fang, Kun Huang, E. Wu, Ming Yan, Heping Zeng
Summary: This article introduces an infrared 3D imaging system capable of detecting three-dimensional structure and reflectivity information. The system operates at single-photon sensitivity and femtosecond timing resolution, enabling imaging under low-light conditions. By nonlinear frequency upconversion and recording with a silicon camera, the presented system achieves high lateral and depth resolutions for 3D reconstruction. Additionally, a numerical denoiser based on spatiotemporal correlation allows the retrieval of object profile and reflectivity even with a detected flux below 0.05 photons/pixel/second. This infrared 3D imager features high detection sensitivity, precise timing resolution, and wide-field operation, offering new possibilities in life and material sciences.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Dermatology
Griffin Lentsch, Erica G. Baugh, Bonnie Lee, Michelle Aszterbaum, Christopher B. Zachary, Kristen M. Kelly, Mihaela Balu
Summary: High-resolution optical imaging technologies, including OCT, RCM, and MPM, have advanced significantly in clinical skin imaging for real-time detection and monitoring of skin diseases, but clinicians need to understand their capabilities and limitations.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Optics
Robbie Murray, Ashley Lyons
Summary: Optical Coherence Tomography (OCT) is a 3D imaging technology that provides micron scale depth resolution, surpassing the millimetre scale typically achieved in Light Detection and Ranging (LiDAR). However, the lack of coherence in LiDAR scenes limits the feasibility of OCT. This paper presents a quantum interference inspired LiDAR approach that achieves OCT depth resolutions without requiring high levels of stability. The enhanced resolution opens doors for LiDAR in various applications.
Article
Biochemical Research Methods
Haigang Ma, Zhongwen Cheng, Zhiyang Wang, Haixia Qiu, Tianding Shen, Da Xing, Ying Gu, Sihua Yang
Summary: The PAMB technology combines photoacoustic microscopy to achieve high-resolution imaging of deep skin tissues, allowing for quantitative assessment of vascular abnormalities and providing valuable information for timely evaluation of treatment response and pathological types of skin diseases.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Optics
Daewoon Seong, Won Ki, Pilun Kim, Jaeyul Lee, Sangyeob Han, Soojin Yi, Hong Kyun Kim, Mansik Jeon, Jeehyun Kim
Summary: This study developed a virtual intraoperative OCT angiography integrated surgical microscope, which can simultaneously visualize the morphological tissue structure and microvasculature data of the surgical region, demonstrating its potential application in in vivo imaging and simulated surgeries of small animals.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Physics, Applied
Haohao Wang, Yuanzheng Ma, Shuxiang Zhao, Yujing Li, Linghua Wu, Huan Qin, Da Xing
Summary: A non-contact thermoacoustic imaging system based on the Fabry-Perot interference principle has been developed, providing high sensitivity and resolution for breast tumor screening.
APPLIED PHYSICS LETTERS
(2021)
Article
Optics
Xiangsen Guo, Guangyuan Ren, Jianbo Tang
Summary: In this study, an autocorrelation analysis-based optical coherence tomography (OCT) method was introduced for accurate measurement of blood flow velocity in the capillary network. The axial blood flow velocity was obtained from the phase change in the decorrelation period of the first-order field autocorrelation function (g1) of the OCT field data. The proposed method showed accurate measurement of axial speed in the range of 0.5-15 mm/s in phantom experiments, and it achieved robust axial velocity measurements with more than five times shorter acquisition time compared to phase-resolved Doppler optical coherence tomography (pr-DOCT) in living animals.
Article
Medicine, General & Internal
Lisa Toto, Maria Ludovica Ruggeri, Federica Evangelista, Chiara Trivigno, Rossella D'Aloisio, Chiara De Nicola, Pasquale Viggiano, Emanuele Doronzo, Marta Di Nicola, Annamaria Porreca, Rodolfo Mastropasqua
Summary: This study investigates the parameters of optical coherence tomography (OCT) and OCT angiography (OCTA) in patients with neovascular age-related macular degeneration (nAMD) and macular neovascularization (MNV) types 1, 2, and 3. The results showed significant differences in CVI, CMT, and ORCCFA between MNV 1 and the other two groups. CVI was significantly different between MNV 1 and healthy controls and between MNV 1 and MNV 2. ORCCFA and CMT were significantly different between MNV 1 and MNV 2. There was no statistically significant difference in subfoveal choroidal thickness (SFCT) among the three groups.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Margaux Schmeltz, Laurianne Robinet, Sylvie Heu-Thao, Jean-Marc Sintes, Claire Teulon, Guillaume Ducourthial, Pierre Mahou, Marie-Claire Schanne-Klein, Gael Latour
Summary: Advanced nonlinear optical microscopy enables quantitative mapping of parchment degradation at the micrometer scale, using two sensitive parameters to distinguish different degradation stages. Validation of this approach on contemporary and historical parchments demonstrates its effectiveness in assessing conservation state and optimizing restoration methods. NLO quantitative microscopy can help identify at-risk parchments and improve restoration processes.
Article
Ophthalmology
Matteo Menean, Riccardo Sacconi, Beatrice Tombolini, Federico Fantaguzzi, Francesco Bandello, Giuseppe Querques
Summary: This study aimed to detect retinal neovascularization elsewhere (NVE), of the optic disc (NVD) and intraretinal microvascular abnormalities (IRMA) in treatment naive diabetic retinopathy (DR) using wide-field colour fundus photography (WF CFP), wide-field spectral-domain optical coherence tomography angiography (OCTA) and the combination of WF CFP and OCTA through overlay software. The results showed that OCTA had high levels of agreement in detecting NVE, NVD and IRMA, and combining WF CFP and OCTA further improved the detection of NVE and NVD.
Article
Physics, Applied
Shiqing Wu, Jing Li, Chao Tao, Xiaojun Liu
Summary: A transmission-mode photoacoustic microscope was developed to achieve quantitative images of two molecular components in tissues. By scanning the specimen once with a single-wavelength laser, a linear equation system was proposed to describe the relationship between signal magnitude and molecular concentrations. This method accurately quantifies concentrations and reveals gradients in mixtures, providing a potential solution to limitations of multiple excitations.
APPLIED PHYSICS LETTERS
(2022)
Article
Optics
Marcin R. Strakowski, Paulina Strakowska, Jerzy Plucinski
Summary: Optical coherence tomography (OCT) is a non-contact and non-invasive method for evaluating semitrans-parent and scattering objects. Spectroscopic analysis applied to OCT measurements can improve the reading of latent fingerprints by enhancing thin film readability. This study describes the combination of spectroscopic analysis and OCT and discusses its benefits for thin film evaluation and fingerprint reading.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Biochemical Research Methods
Steve Frisken, Trevor Anderson, Armin Segref, Dirk Lorenser, Grant Frisken
Summary: HP-OCT is a parallel spectral domain imaging technology that is well-suited for imaging the anterior segment of the eye. This study demonstrates that sparsely sampled volumes can be registered without active eye tracking to produce motion artifact-free 3D volumes. The anterior volume provides comprehensive 3D biometric information, including lens position, curvature, epithelial thickness, tilt, and axial length. Moreover, by changing a detachable lens, high-resolution anterior volumes and posterior volume images for preoperative assessment of the posterior segment can be captured. Importantly, the retinal volumes have the same Nyquist range as the anterior imaging mode.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemical Research Methods
Gregory N. McKay, Ryan C. Niemeier, Carlos Castro-Gonzalez, Nicholas J. Durr
Summary: The recent application of modern microscopy techniques to human capillary imaging holds great promise for non-invasive alternatives to conventional blood tests. Techniques such as oblique plane microscopy and scattering-contrast oblique plane microscopy have been demonstrated to effectively image blood cells in human capillaries, offering a new avenue for in vivo blood analysis.
BIOMEDICAL OPTICS EXPRESS
(2021)
Review
Medicine, General & Internal
Yong Li, Feihui Zheng, Li Lian Foo, Qiu Ying Wong, Daniel Ting, Quan V. Hoang, Rachel Chong, Marcus Ang, Chee Wai Wong
Summary: Advancements in optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA) technology have greatly improved the understanding, diagnosis, and treatment of myopia and myopia-related complications. These imaging techniques provide detailed visualization of various parts of the eye and have led to the development of new classification systems and treatment guidelines. Additionally, the application of artificial intelligence in myopia imaging shows promising results.
Article
Optics
I Astrauskas, B. Povazay, A. Baltuska, A. Pugzlys
Summary: The developed Ho:YAG chirped pulse amplifier, suitable for material processing applications, produces high-energy pulses with simple dispersion management, as both pulse stretching and compression are realized within a single chirped volume Bragg grating. Through studying laser ablation of a gold layer, it was found that short pulse durations are most suitable for achieving non-thermal ablation.
OPTICS AND LASER TECHNOLOGY
(2021)
Article
Biology
Nicole Gruenheit, Amy Baldwin, Balint Stewart, Sarah Jaques, Thomas Keller, Katie Parkinson, William Salvidge, Robert Baines, Chris Brimson, Jason B. Wolf, Rex Chisholm, Adrian J. Harwood, Christopher R. L. Thompson
Summary: In this study, REMI-seq, an adaptation of Tn-seq, is described as a powerful tool for high throughput gene function analysis in the model system Dictyostelium discoideum. The method allows quantitative identification of genomic insertion sites of a drug resistance marker, enabling efficient linkage between genotype and phenotype. The resources developed using REMI-seq, including a near genome-wide resource of individual mutants and a defined pool of 'barcoded' mutants, facilitate rapid identification of novel regulators of cellular processes.
Article
Engineering, Electrical & Electronic
Marco Andreana, Caterina Sturtzel, Ming Yang, Richard Latham, Rainer A. Leitgeb, Wolfgang Drexler, Manuel Zimmer, Tuan Le, Martin Distel, Angelika Unterhuber
Summary: This paper introduces a triple-modal multicolor MPM platform using a single direct diode-pumped femtosecond Kerr-lens-mode-locked Ti:sapphire oscillator to simultaneously acquire multiple signals. The laser reduces complexity, footprint, and cost, making it suitable for biomedical applications. In vivo imaging of C. elegans and metabolic changes in zebrafish larvae are successfully demonstrated using this platform.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Gareth Chapman, Mouhamed Alsaqati, Sharna Lunn, Tanya Singh, Stefanie C. Linden, David E. J. Linden, Marianne B. M. van den Bree, Mike Ziller, Michael J. Owen, Jeremy Hall, Adrian J. Harwood, Yasir Ahmed Syed
Summary: Copy Number Variation (CNV) at the 1q21.1 locus is associated with a range of neurodevelopmental and psychiatric disorders in humans, with deletion or duplication of this locus leading to reciprocal phenotypes in neuronal development. These differences are also conserved in a mouse model of 1q21.1 deletion, indicating potential for drug interventions through targeting calcium channels in neurons with 1q21.1 deletion or duplication.
MOLECULAR PSYCHIATRY
(2022)
Article
Oncology
Gabriel Giardina, Alexander Micko, Daniela Bovenkamp, Arno Krause, Fabian Placzek, Laszlo Papp, Denis Krajnc, Clemens P. Spielvogel, Michael Winklehner, Romana Hoeftberger, Greisa Vila, Marco Andreana, Rainer Leitgeb, Wolfgang Drexler, Stefan Wolfsberger, Angelika Unterhuber
Summary: The pituitary gland governs the function of many endocrine glands and pituitary oncogenesis can disrupt hormonal balance. A multimodal imaging approach, including techniques like OCT, MPM, and LSRM, allows for discrimination of pituitary gland and adenomas. By analyzing chemical compounds and their spatial distribution, relevant biomarkers for pituitary adenomas can be identified and classified with high accuracy.
Article
Cell Biology
Marco Andreana, Caterina Sturtzel, Clemens P. Spielvogel, Laszlo Papp, Rainer Leitgeb, Wolfgang Drexler, Martin Distel, Angelika Unterhuber
Summary: The study utilized coherent anti-Stokes Raman scattering microscopy to investigate lipid accumulation in living zebrafish larvae developing tumors, revealing higher lipid accumulation in oncogene-expressing larvae compared to healthy ones. This high lipid accumulation may reflect an altered lipid metabolism in the hyperproliferating oncogene-expressing cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Rainer Leitgeb, Fabian Placzek, Elisabet Rank, Lisa Krainz, Richard Haindl, Qian Li, Mengyang Liu, Marco Andreana, Angelika Unterhuber, Tilman Schmoll, Wolfgang Drexler
Summary: After three decades of development, optical coherence tomography (OCT) has become one of the most successful imaging technologies with significant clinical and economic impacts. Future innovations in OCT will focus on enhancing imaging resolution and speed, as well as exploring new applications such as molecular and metabolic imaging. OCT has revolutionized ophthalmic diagnosis and has great potential for growth in both academic research and industry.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Analytical
Leopold Veselka, Lisa Krainz, Leonidas Mindrinos, Wolfgang Drexler, Peter Elbau
Summary: Optical coherence tomography (OCT) is a widely used imaging technique in the micrometer regime that has gained interest in medical imaging over the past twenty years. A quantitative model has been proposed in this study for a more realistic description of the OCT imaging process, taking into account system relevant parameters. The accuracy of the model has been illustrated through simulations and validated with experimental data from a 1300 nm swept-source OCT system after calibration of necessary system parameters.
Article
Multidisciplinary Sciences
Bret Sanders, Daniel D'Andrea, Mark O. Collins, Elliott Rees, Tom G. J. Steward, Ying Zhu, Gareth Chapman, Sophie E. Legge, Antonio F. Pardinas, Adrian J. Harwood, William P. Gray, Michael C. O'Donovan, Michael J. Owen, Adam C. Errington, Derek J. Blake, Daniel J. Whitcomb, Andrew J. Pocklington, Eunju Shin
Summary: The coordinated programs of gene expression driving brain development have been studied in relation to neuropsychiatric disorders and cognition. This research combines human genetics with transcriptomic data and analyzes genetic variants and GWAS statistics to reveal the convergence on transcriptional programs regulating excitatory cortical neurogenesis.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Balint Stewart, Nicole Gruenheit, Amy Baldwin, Rex Chisholm, Daniel Rozen, Adrian Harwood, Jason B. Wolf, Christopher R. L. Thompson
Summary: This study investigates natural variation in predatory performance in the microbial predator Dictyostelium discoideum. The researchers found widespread nontransitive differences among strains in predatory success across different bacterial prey, which can facilitate strain coexistence in multi-prey environments. Furthermore, most mutations had prey-specific effects, highlighting the potential for prey-specific effects to weaken selection and prevent the emergence of an optimal generalist predator.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
David Reichert, Mikael T. Erkkilae, Johanna Gesperger, Lisa I. Wadiura, Alexandra Lang, Thomas Roetzer-Pejrimovsky, Adelheid Woehrer, Marco Wilzbach, Christoph Hauger, Wolfgang Drexler, Barbara Kiesel, Georg Widhalm, Rainer A. Leitgeb, Angelika Unterhuber, Marco Andreana
Summary: Precise intraoperative brain tumor visualization is crucial for surgeons to achieve maximal safe resection. Recent studies have shown that protoporphyrin IX fluorescence-guided surgery can improve prognosis in patients with high-grade gliomas. This work demonstrates further optimization of the technique by minimizing the crosstalk of autofluorescence signal contributions, resulting in a clearer distinction between tumor fluorescence and autofluorescence background.
APPLIED SCIENCES-BASEL
(2022)
Article
Endocrinology & Metabolism
Alexander Micko, Fabian Placzek, Roger Fonolla, Michael Winklehner, Ryan Sentosa, Arno Krause, Greisa Vila, Romana Hoeftberger, Marco Andreana, Wolfgang Drexler, Rainer A. Leitgeb, Angelika Unterhuber, Stefan Wolfsberger
Summary: The study successfully demonstrated the objective identification of pituitary adenoma tissue in OCT images using a combination of OCT imaging and CNN in an ex vivo setting, with high sensitivity. However, further investigation with larger sample sizes is needed to validate the findings.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Psychiatry
Mouhamed Alsaqati, Brittany A. Davis, Jamie Wood, Megan M. Jones, Lora Jones, Aishah Westwood, Olena Petter, Anthony R. Isles, David Linden, Marianne Van den Bree, Michael Owen, Jeremy Hall, Adrian J. Harwood
Summary: Genetic evidence suggests that disrupted epigenetic regulation is a major risk factor for psychiatric disorders. This study reveals that EHMT1, an epigenetic repressor, indirectly regulates NRSF/REST through repression of miRNA, leading to abnormal neuronal gene expression and neurodevelopment timing. The findings highlight the broad molecular interaction between H3K9 demethylation, NSRF/REST regulation, and the risk for intellectual disability and schizophrenia.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Engineering, Electrical & Electronic
Lisa Krainz, Ekaterina Sherina, Simon Hubmer, Mengyang Liu, Wolfgang Drexler, Otmar Scherzer
Summary: Optical coherence elastography (OCE) is a promising research field for visualizing micro-scale tissue stiffness changes. This study compares three different reconstruction methods for accurately determining Young's modulus, with the image-based inverse reconstruction method (IIM) showing the best performance.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
