Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 32, Issue 5, Pages 701-708Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2013.788983
Keywords
tobacco mosaic virus; spherical particles; amyloid structures.; alpha-and beta-structures; thermal remodeling
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Funding
- Ministry of Education and Sciences of Russia [8564]
- Russian Foundation for Basic Research [12-04-01472-a]
- M.V. Lomonosov Moscow State University
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Conversion of the rod-like tobacco mosaic virus (TMV) virions into ball-like particles by thermal denaturation at 90-98 degrees C had been described by R.G. Hart in 1956. We have reported recently that spherical particles (SPs) generated by thermal denaturation of TMV at 94-98 degrees C were highly stable, RNA-free, and water-insoluble. The SPs were uniform in shape but varied widely in size (53-800 nm), which depended on the virus concentration. Here, we describe some structural characteristics of SPs using circular dichroism, fluorescence spectroscopy, and Raman spectroscopy. It was found that the structure of SPs protein differs strongly from that of the native TMV and is characterized by coat protein subunits transition from mainly (about 50%) alpha-helical structure to a structure with low content of alpha-helices and a significant fraction of beta-sheets. The SPs demonstrate strong reaction with thioflavin T suggesting the formation of amyloid-like structures.
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