Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 19, Issue 11, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.19.11.116004
Keywords
fluorescence lifetime imaging; two-photon excited fluorescence; acousto-optic deflector; random regions of interest
Funding
- National Basic Research Program of China [2015CB352005, 2012CB825802]
- National Natural Science Foundation of China [61378091, 11204226, 61405123]
- Shenzhen Science and Technology Development Project [ZDSY20120612094247920, JCYJ20130329114905559, CXB201104220021A]
- China Postdoctoral Science Foundation [2014M552226]
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We report a dynamic fluorescence lifetime imaging (D-FLIM) system that is based on a pair of acousto-optic deflectors for the random regions of interest (ROI) study in the sample. The two-dimensional acousto-optic deflector devices are used to rapidly scan the femtosecond excitation laser beam across the sample, providing specific random access to the ROI. Our experimental results using standard fluorescent dyes in live cancer cells demonstrate that the D-FLIM system can dynamically monitor the changing process of the microenvironment in the ROI in live biological samples. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
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