Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 19, Issue 10, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.19.10.108002
Keywords
biophysical stimulation; bone remodeling; diode laser; in vitro study; osteoblast-like cells
Funding
- Rizzoli Orthopaedic Institute (Ricerca Corrente)
- Rizzoli Orthopaedic Institute-RIT [POR-FESR 2007-2013]
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Because osteoblasts play a key role in bone remodeling and the influence of low-level laser therapy on this process is not clear, Saos-2 human osteoblast-like cells were irradiated by a gallium-aluminum-arsenide diode laser (915 nm) for 10, 48, 96, 193, and 482 s using doses 1, 5, 10, 20, and 50 J/cm(2), respectively. A control group was not irradiated. Morphology, viability, and cytotoxicity analyses were carried out after 1 hr, 1 day, and 3 days. Deoxyribose nucleic acid (DNA) content and release of vascular endothelial growth factor (VEGF), receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) were evaluated. Viability was modulated by laser irradiation in a dose-dependent manner, with 10 J/cm(2) inducing a biostimulatory response and 20 to 50 J/cm(2) determining a bioinhibitory and cytotoxic effect. Accordingly, DNA content was generally increased for the 10 J/cm(2) dose and decreased for the 50 J/cm(2) dose. A rapid and transitory trend toward increased RANKL/OPG ratio and a tendency toward a delayed increase in VEGF release for doses of 1 to 10 J/cm(2) was found. Further investigations using the biostimulatory dose of 10 J/cm(2) emerged from this study are needed to establish the ideal treatment regimens in the laboratory as well as in clinical practice. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
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