Article
Biochemical Research Methods
Janet A. H. Tang, Charles E. Granger, Karteek Kunala, Keith Parkins, Khang T. Huynh, Kristen Bowles-Johnson, Qiang Yang, Jennifer J. Hunter
Summary: This article introduces a new method that combines adaptive optics technology with fluorescence lifetime detection to investigate functional and compositional differences within the eye and between subjects. The new adaptive optics fluorescence lifetime imaging ophthalmoscope was successfully demonstrated in 6 subjects, with safety testing indicating no concerns.
BIOMEDICAL OPTICS EXPRESS
(2022)
Review
Ophthalmology
Lydia Sauer, Alexandra S. Vitale, Natalie K. Modersitzki, Paul S. Bernstein
Summary: FLIO is a noninvasive imaging modality based on the Heidelberg Engineering Spectralis system, which has shown to provide additional information to conventional imaging modalities in different retinal diseases such as AMD, MacTel, and Stargardt disease. It has the potential to detect early signs of hydroxychloroquine toxicity and more benefits that are yet to be discovered.
Article
Ophthalmology
Maarjaliis Paavo, Winston Lee, Rait Parmann, Jose Ronaldo Lima de Carvalho Jr, Jana Zernant, Stephen H. Tsang, Rando Allikmets, Janet R. Sparrow
Summary: In this study, the features of PROM1-macular dystrophy were described using multimodal images. The results showed that areas of granular deposition in SW-AF and NIR-AF corresponded to loss of the ellipsoid zone and outer retinal layers. The qAF approach could be used for early differential diagnosis and to identify appropriate disease targets.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Ophthalmology
Ekaterina Loskutova, John S. Butler, Gabriela Hernandez Martinez, Ian Flitcroft, James Loughman
Summary: The study aimed to investigate the effect of pupillary dilation on MP measurement using dual-wavelength AF. Results showed that pupillary dilation significantly changed MPOD values, and these changes were not uniform across the spatial profile.
CURRENT EYE RESEARCH
(2021)
Article
Oncology
Maria Lukina, Konstantin Yashin, Elena E. Kiseleva, Anna Alekseeva, Varvara Dudenkova, Elena V. Zagaynova, Evgenia Bederina, Igor Medyanic, Wolfgang Becker, Deependra Mishra, Mikhail Berezin, Vladislav I. Shcheslavskiy, Marina Shirmanova
Summary: The development of a rapid and sensitive assay for intraoperative diagnostics of glioma, focusing on fluorescence lifetime imaging (FLIM) to identify imaging markers for differentiation between tumors and healthy tissues, shows a high potential for diagnostics of glioma and evaluation of surgical margins.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Johannes Birtel, Tobias Bauer, Laurenz Pauleikhoff, Theodor Rueber, Martin Gliem, Peter Charbel Issa
Summary: Retinal disease and aging are closely related to lipofuscin, a complex of fluorescent metabolites. The use of red excitation light in fundus autofluorescence (R-AF) imaging overcomes the limitations of short-wavelength excitation light, providing high-quality images and uncovering previously undetected disease features. R-AF signal intensity correlates with lipofuscin abundance and is not affected by photopigment bleaching or macular pigment. R-AF imaging is a promising tool for routine clinical monitoring in retinal diseases such as age-related macular degeneration and Stargardt disease.
SCIENTIFIC REPORTS
(2023)
Review
Ophthalmology
Andrew X. Chen, Thais F. Conti, Grant L. Hom, Tyler E. Greenlee, Raffaele Raimondi, Isaac N. Briskin, Collin A. Rich, Reecha Kampani, Robert Engel, Sumit Sharma, Katherine E. Talcott, Rishi P. Singh
Summary: Mitochondria play a critical role in cellular energy production and homeostasis, with oxidative stress and associated mitochondrial dysfunction being integral components of various retinal diseases. Recent research has focused on utilizing oxidized FPF as a biomarker for mitochondrial dysfunction, with multiple generations of retinal FPF imaging devices already developed for this purpose.
Article
Ophthalmology
Martin Hammer, Juliane Jakob-Girbig, Linda Schwanengel, Christine A. Curcio, Somar Hasan, Daniel Meller, Rowena Schultz
Summary: The study observed changes in the retinal pigment epithelium (RPE) during the transition from dysmorphia to atrophy in age-related macular degeneration (AMD) using fluorescence lifetime imaging ophthalmoscopy (FLIO). The findings showed that as hyperpigmented areas form and RPE migrates into the retina, fluorescence lifetimes lengthen and emission spectrum intensity ratio (ESRI) of RPE cells increase, suggesting potential early biomarkers for AMD progression.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Ophthalmology
Jeong Hyun Lee, Jeeyun Ahn, Joo Young Shin
Summary: The study investigated the temporal order of photoreceptor atrophy, retinal pigment epithelium (RPE) atrophy, and visual acuity loss in patients with center-involving geographic atrophy (GA) in non-exudative age-related macular degeneration (neAMD). The findings showed that photoreceptor atrophy on OCT and RPE atrophy on FAF preceded visual decline in the progression of center-involving GA.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Linda-Sophia Schwanengel, Sebastian Weber, Rowena Simon, Thomas Lehmann, Regine Augsten, Daniel Meller, Martin Hammer
Summary: This study observed the FAF lifetimes and PEW of drusen in AMD patients and compared them to the pathology of the overlying RPE. It was found that the FAF lifetimes and PEW of drusen changed over the progression of AMD, which may reflect dysmorphia of the RPE. These findings suggest that FLIO could be a useful tool for early detection of AMD progression risk.
ACTA OPHTHALMOLOGICA
(2023)
Article
Biochemical Research Methods
Martin Hammer, Rowena Simon, Daniel Meller, Matthias Klemm
Summary: Fluorescence lifetime imaging ophthalmoscopy provides valuable information for detecting ocular diseases and monitoring disease progression by analyzing fluorescence lifetimes and peak emission wavelengths. It can identify potentially pathological fundus areas by comparing them to a normal range, and changes in fluorescence parameters can be traced over time.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Ophthalmology
Leon von der Emde, Robyn H. Guymer, Maximilian Pfau, Emily Caruso, Pyrawy Sivarajah, Lauren A. B. Hodgson, Myra B. McGuinness, Kenneth R. Sloan, Zhichao Wu
Summary: The study found that there were no significant differences in qAF values between eyes with large drusen and healthy eyes, but qAF values showed a significant decline over time in eyes with age-related macular degeneration. Additional research is needed to better understand the relationship between qAF levels and retinal pigment epithelium health.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2021)
Article
Ophthalmology
Liang Han, Jose Ronaldo Lima de Carvalho, Rait Parmann, Tongalp H. Tezel, Stanley Chang, Tarun Sharma, Janet R. Sparrow
Summary: qAF was correlated with SD-OCT detectable features in CSC patients, while NIR-AF images were helpful in detecting lesions. Elevated qAF levels were associated with specific CSC-related abnormalities, indicating a potential role in disease progression.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Ophthalmology
Andreas Berlin, Mark E. Clark, Thomas A. Swain, Nathan A. Fischer, Gerald McGwin, Kenneth R. Sloan, Cynthia Owsley, Christine A. Curcio
Summary: This study investigates the use of quantitative autofluorescence (qAF8) in patients with and without early or intermediate age-related macular degeneration (AMD), taking into account the impact of the aged crystalline lens and posterior capsular opacification (PCO). The results suggest that qAF8 is not associated with AMD status, up to intermediate AMD, when considering only pseudophakic eyes to avoid noisy images in phakic eyes. In pseudophakic eyes, qAF8 is not affected by PCO.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2022)
Article
Ophthalmology
Akira Obana, Kibo Ote, Fumio Hashimoto, Ryo Asaoka, Yuko Gohto, Shigetoshi Okazaki, Hidenao Yamada
Summary: The study found that using deep learning technology can effectively correct measured MPOD values in eyes with cataract, reducing errors significantly.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)