Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 18, Issue 6, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.18.6.061230
Keywords
resonance Raman spectroscopy; Raman microscopy; reflection spectroscopy; skin color measurements; antioxidants
Funding
- Foundation Skin Physiology of the Donor Association for German Science and Humanities
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Carotenoids are important substances for human skin due to their powerful antioxidant properties in reaction of neutralization of free radicals and especially reactive oxygen species, including singlet oxygen. Concentration of carotenoids in the skin could mirror the current redox status of the skin and should be investigated in vivo. Optical methods are ideally suited for determination of carotenoids in mammalian skin in vivo as they are both noninvasive and quick. Four different optical methods could be used for in vivo measurement of carotenoids in the human or animal skin: (1) resonance Raman spectroscopy; (2) Raman microscopy; (3) reflection spectroscopy; (4) skin color measurements. The advantages, shortcomings, and limitations of the above-mentioned optical methods are discussed. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
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