Article
Oncology
Avanti Verma, Barbara Burtness
Summary: Advances in treatment deintensification for human papillomavirus-associated oropharyngeal cancer include the use of transoral surgery to reduce the need for postoperative therapy. Trials have shown high progression-free survival rates for intermediate and favorable risk patients treated with radiation or chemoradiation. Immunotherapy shows promise in first-line treatment of recurrent and metastatic head and neck cancer, particularly in patients with nasopharyngeal cancer.
Review
Otorhinolaryngology
Ruwaa Samarrai, Samantha Frank, Avery Lum, Kristina Woodis, George Weinstock, Daniel Roberts
Summary: This paper aims to define the microbiome of the head and neck by characterizing and distinguishing commensal bacteria from pathogenic species. A literature review found limited studies on isolated anatomic subsites in the micro environments of the head and neck, highlighting the need for further research. Understanding the microbiome of the head and neck can help differentiate disease-prone patients, guide treatment regimens, and limit adverse effects of antibiotic overuse.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2022)
Article
Oncology
Nicholas C. J. Lee, Antoine Eskander, Joseph A. Miccio, Henry S. Park, Chirag Shah, Michael Rutenberg, Ali Hosni, Zain A. Husain
Summary: This study conducted multivariable analysis on HN-STS patients in the SEER database, showing that tumor invasion of adjacent structures and increasing de-differentiation were associated with poorer survival. The stage grouping system established using RPA was successfully validated in the NCDB cohort.
Editorial Material
Medicine, General & Internal
Lukas D. Landegger
Summary: Tuberculosis is a global health challenge that primarily affects developing countries. Doctors worldwide need to be able to recognize the diverse clinical manifestations of the disease in order to provide appropriate treatment. This article discusses the less common presentation of isolated abscesses in the head and neck region caused by tuberculosis, which can lead to symptoms such as difficulty swallowing and voice changes. Characteristic imaging findings and potential surgical options are also explored.
Article
Oncology
Linda Sharp, Laura-Jayne Watson, Liya Lu, Sam Harding, Katrina Hurley, Steve J. Thomas, Joanne M. Patterson
Summary: This study investigated cancer-related fatigue (CRF) in head and neck cancer patients using data from the Head and Neck 5000 prospective clinical cohort. The findings revealed a high prevalence of CRF, indicating the need for additional interventions and supports for affected patients.
Article
Oncology
Chih-Wei Yang, Yueng-Hsiang Chu, Hsin-Chien Chen, Wei-Chen Huang, Peng-Jen Chen, Wei-Kuo Chang
Summary: UGI endoscopy with oral-pharynx-larynx examination can achieve opportunistic head and neck cancer screening and is recommended for every patient in endoscopy units.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Claudia Maletzki, Vivica Freiin Grote, Friederike Kalle, Thoralf Kleitke, Annette Zimpfer, Anne-Sophie Becker, Wendy Bergmann-Ewert, Anika Jonitz-Heincke, Rainer Bader, Brigitte Vollmar, Stephan Hackenberg, Agmal Scherzad, Robert Mlynski, Daniel Strueder
Summary: High hydrostatic pressure of at least 450 MPa can safely devitalize head and neck cancer cells and tissues, and may be used to enhance the development of tumor vaccines and functional reconstruction. Further research is needed to determine the precise thresholds for safe cancer cell killing.
CELL DEATH DISCOVERY
(2023)
Review
Medicine, General & Internal
Fernando Lopez, Antti Makitie, Remco de Bree, Alessandro Franchi, Pim de Graaf, Juan C. Hernandez-Prera, Primoz Strojan, Nina Zidar, Margareta Strojan Flezar, Juan P. Rodrigo, Alessandra Rinaldo, Barbara A. Centeno, Alfio Ferlito
Summary: Diagnosis plays a crucial role in determining the nature of a disease, with qualitative diagnosis by pathologists and quantitative diagnosis by radiologists being important in the management of head and neck tumors. Advances in immunohistochemistry and molecular biology have allowed for more precise diagnoses, while imaging techniques enable radiologists to estimate tumor volume and extent. Accurate diagnosis is essential for treatment planning and improving patient outcomes.
Article
Oncology
Chikatoshi Katada, Manabu Muto, Satoshi Fujii, Tetsuji Yokoyama, Tomonori Yano, Akihito Watanabe, Toshiro Iizuka, Shigetaka Yoshinaga, Ichiro Tateya, Hiroki Mitani, Yuichi Shimizu, Akiko Takahashi, Tomoyuki Kamijo, Noboru Hanaoka, Makoto Abe, Akihiro Shiotani, Koichi Kano, Yukinori Asada, Tamotsu Matsuhashi, Hirohito Umeno, Kenji Okami, Kenichi Goda, Shinichiro Hori, Yoichiro Ono, Shuji Terai, Yasuaki Nagami, Kenichi Takemura, Kenro Kawada, Mizuo Ando, Naoto Shimeno, Akihito Arai, Yasutoshi Sakamoto, Masaaki Ichinoe, Tetsuo Nemoto, Masahiro Fujita, Hidenobu Watanabe, Tadakazu Shimoda, Atsushi Ochiai, Takakuni Kato, Ryuichi Hayashi
Summary: The study examined the safety and efficacy of transoral surgery for superficial head and neck cancer, finding that the procedure was effective with relatively low incidence of adverse events and no treatment-related deaths, indicating it as an effective minimally invasive treatment option.
Article
Medicine, Research & Experimental
Craig A. Bollig, Christopher I. Newberry, Tabitha L. I. Galloway, Robert P. Zitsch, Elyse K. Hanly, Vivian L. Zhu, Nitin Pagedar, Rohit Nallani, Andres M. Bur, William C. Spanos, Jeffrey B. Jorgensen
Summary: The site and pattern of distant metastasis were found to impact overall survival in head and neck cancer patients, with bone and polymetastatic disease associated with poorer prognosis.
Article
Multidisciplinary Sciences
Yen-Ting Lu, Chung-Han Hsin, Ying-Chou Lu, Meng-Che Wu, Jing-Yang Huang, Cheng-Chen Huang, Po-Hui Wang, Shun-Fa Yang
Summary: It remains inconclusive whether patients with peptic ulcer disease are at a higher risk of head and neck cancer. An observational study found that patients with peptic ulcer disease had a higher incidence rate of head and neck cancer, particularly laryngeal and hypopharyngeal cancer. However, in patients receiving H. pylori treatment, the risk of head and neck cancer was similar to that of the control group.
SCIENTIFIC REPORTS
(2021)
Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Article
Oncology
Ryuhei Okada, Aki Furusawa, Fuyuki Inagaki, Hiroaki Wakiyama, Takuya Kato, Shuhei Okuyama, Hideyuki Furumoto, Hiroshi Fukushima, Peter L. Choyke, Hisataka Kobayashi
Summary: The study confirmed the feasibility of endoscopic NIR-PIT for treating head and neck cancer, with quantitative fluorescence endoscopic imaging being used to observe and treat the cancer.
Article
Oncology
Hanya Mahmood, Muhammad Shaban, Nasir Rajpoot, Syed A. Khurram
Summary: This paper reviews recent literature on the use of Artificial Intelligence/Machine Learning (AI/ML) methods for diagnostic evaluation of head and neck cancers (HNC) through automated image analysis. The studies show that these methods can achieve high accuracy in detection by using various imaging modalities.
BRITISH JOURNAL OF CANCER
(2021)
Article
Medicine, Research & Experimental
Peter Filip, David K. Lerner, Evan Kominsky, Alexander Schupper, Katherine Liu, Nazir Mohemmed Khan, Scott Roof, Constantinos Hadjipanayis, Eric Genden, Alfred M. C. Iloreta
Summary: This study investigates the use of 5-aminolevulinic acid (5-ALA) fluorescence for resection of head and neck squamous cell carcinoma (HNSCC), which can demonstrate positive margin, perineural invasion, and lymph node metastasis. Although safety conclusions are lacking for this drug in the head and neck cancer population, our study aligns with extensive safety data in the neurosurgical literature. Future applications may include intraoperative assessment of margin status, diagnostic accuracy, and impacts on survival.
Article
Chemistry, Analytical
Kideog Bae, Le Xin, Wei Zheng, Carol Tang, Beng-Ti Ang, Zhiwei Huang
Summary: Recent genomic studies have paved the way for effective clinical treatments of glioblastoma (GBM) subtypes, but intratumoral heterogeneity complicates subtype identification. The use of hyperspectral stimulated Raman scattering (SRS) microscopy allows for rapid, label-free molecular assessment of GBM intratumoral heterogeneity. By developing a unique SRS imaging diagnostic platform, it is possible to accurately determine tumor heterogeneity in GBM tissues, thus potentially improving targeted therapy for GBM in the future.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chi Shu, Wei Zheng, Kan Lin, Chweeming Lim, Zhiwei Huang
Summary: This study applies fiberoptic Raman endoscopy to conduct follow-up surveys and accurate detection of tumor recurrence in post-treatment NPC patients. The Raman endoscopy shows significant spectral differences among normal tissue, NPC, and nonrecurring post-treatment patients, with high diagnostic accuracy for distinguishing recurrent NPC from post-treatment inflammation and fibrosis. This work demonstrates the clinical potential of fiberoptic Raman endoscopy as a powerful diagnostic tool for post-treatment surveying and recurrent tumor detection at the molecular level.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Conor C. Horgan, Mads S. Bergholt, May Zaw Thin, Anika Nagelkerke, Robert Kennedy, Tammy L. Kalber, Daniel J. Stuckey, Molly M. Stevens
Summary: This study aims to extend Raman spectroscopic diagnostics and develop a multimodal computer vision-based diagnostic system for the detection and precise delineation of tumor margins. The system enables real-time tumor margin delineation and has the potential to improve clinical tumor resection surgeries.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Analytical
Chi Shu, Hanshu Yan, Wei Zheng, Kan Lin, Anne James, Sathiyamoorthy Selvarajan, Chwee Ming Lim, Zhiwei Huang
Summary: This study developed a deep learning-guided fiberoptic Raman diagnostic platform, with the optimized RS-CNN model showing good diagnostic accuracy and sensitivity in real-time NPC diagnosis, outperforming traditional linear discriminate analysis methods. By investigating saliency maps of the RS-CNN models, the diagnostic capability related to biomolecular variations was validated.
ANALYTICAL CHEMISTRY
(2021)
Article
Orthopedics
Kimberly R. Kroupa, Man Wu, Juncheng Zhang, Magnus Jensen, Wei Wong, Julie B. Engiles, Mark W. Grinstaff, Brian D. Snyder, Mads S. Bergholt, Michael B. Albro
Summary: This study introduces a novel arthroscopic Raman probe for optical biopsy of cartilage and quantification of ECM biomarkers, which can help determine cartilage properties in health and disease. The Raman GAG score biomarkers predicted GAG content variation in different cartilage models, and the RCAF values were useful in assessing COL loss. Multivariate linear regression of Raman-derived ECM biomarkers showed promise in predicting cartilage modulus variation. In vivo Raman arthroscopy assessment was successfully performed on an ovine femoral condyle, showing the potential of this technology in monitoring treatment outcomes for osteoarthritis therapies.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Optics
Li Gong, Shulang Lin, Zhiwei Huang
Summary: A novel z-scan-free stimulated Raman scattering tomography (SRST) utilizing optical beating technique (OBT) and Bessel beams enables deeper penetration for label-free volumetric chemical imaging with subcellular resolution. The technology encodes depth-resolved SRS signals in the spatial frequency domain, rapidly retrieving volumetric tissue information without mechanical depth-scanning, and demonstrates improved imaging depth in highly scattering samples compared to conventional SRS microscopy, with potential applications in various biological and biomedical systems.
LASER & PHOTONICS REVIEWS
(2021)
Article
Optics
Chi Shu, Wei Zheng, Zhuo Wang, Changyuan Yu, Zhiwei Huang
Summary: The study introduces a disposable submillimeter fiber optic Raman needle probe for enhancing real-time in vivo tissue and biofluids Raman measurements. By using the probe and structured background subtraction algorithms, high quality Raman spectra from various tissue types and biofluids can be rapidly obtained. This technique shows promise in achieving real-time deep tissue and biofluids diagnosis and characterization in different organs.
Article
Chemistry, Analytical
Conor C. Horgan, Magnus Jensen, Anika Nagelkerke, Jean-Philippe St-Pierre, Tom Vercauteren, Molly M. Stevens, Mads S. Bergholt
Summary: By utilizing deep learning, denoising and reconstructing low signal-to-noise ratio Raman molecular signatures is achieved, with a 10x improvement in mean-squared error over common Raman filtering methods. A neural network for robust 2-4X spatial super-resolution of hyperspectral Raman images is developed, resulting in Raman imaging speed-ups of up to 40-90X, enabling good-quality cellular imaging in under 1 min. Transfer learning is then applied to extend DeepeR from cell to tissue-scale imaging, providing a foundation for higher-throughput Raman spectroscopy and molecular imaging applications across biomedicine.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Conor C. Horgan, Magnus Jensen, Ciro Chiappini, T. O. M. Vercauteren, Richard Cook, Mads S. Bergholt
Summary: This article presents a novel hybrid fiber-optic confocal Raman endomicroscopy system that allows for simultaneous imaging and analysis of tissue morphology and chemical composition, paving the way for multimodal diagnostics during endoscopy.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Optics
Xiaobo Lv, Li Gong, Shulang Lin, Peng Jin, Zhiwei Huang
Summary: We have developed a unique super-resolution stimulated Raman scattering (SRS) microscopy technique called phase-shifted spatial frequency modulation (PSFM) under widefield illumination. This technique enables super-resolution chemical imaging with single-pixel detection. By projecting pump and Stokes laser patterns with varying spatial frequencies onto the sample and combining with a proposed phase shift, higher spatial information can be rapidly retrieved using fast inverse Fourier-transform. This technique provides approximately 2.2-fold improvement in spatial resolution compared to conventional point-scan SRS microscopy, while requiring much reduced laser excitation power density, suggesting its potential for label-free super-resolution chemical imaging in cells and tissue.
Article
Optics
Howard Peng Sin Heng, Chi Shu, Wei Zheng, Zhiwei Huang
Summary: We report the development of a novel coaxial double-clad-fiber (DCF) and graded-index (GRIN) fiberoptic Raman probe for enhancing in vivo epithelial tissue Raman measurements. The probe can acquire high-quality Raman spectra from various oral tissues within sub-seconds and detect subtle biochemical differences between different epithelial tissues, suggesting its potential for in vivo diagnosis and characterization.
Article
Chemistry, Analytical
Chi Shu, Wei Zheng, Kan Lin, Chwee Ming Lim, Zhiwei Huang
Summary: This study demonstrates the ability of rapid fiberoptic Raman endoscopy to stage and diagnose nasopharyngeal cancer in real-time. By analyzing Raman spectra of tissue sites, a staging model is established with high accuracy. The findings show that fiberoptic Raman endoscopy is a robust tool for clinical staging of nasopharyngeal cancer.
Article
Optics
Shulang Lin, Li Gong, Zhiwei Huang
Summary: This article reports a novel two-photon fluorescence tomography (TPFT) technique that enables super-resolution deeper tissue 3D imaging without the need for z-scanning. By using phase-shifted optical beatings of Bessel beams, the lateral and axial resolutions are improved, and the penetration depth is increased. This method allows for depth-resolved imaging without mechanical depth-scanning.
LASER & PHOTONICS REVIEWS
(2023)
Article
Automation & Control Systems
Karthik Prathaban, Bingcheng Wu, Char Loo Tan, Zhiwei Huang
Summary: This study developed a robust multilayer sigmoid-activated convolutional neural network (MLS-CNN) for accurately delineating infiltrative brain tumor margins in glioma histology. The MLS-CNN achieved high diagnostic accuracy and efficiency, and its classifications were strongly correlated with gene expression levels.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Medicine, Research & Experimental
Kideog Bae, Wei Zheng, Zhiwei Huang