Article
Multidisciplinary Sciences
Aditi Sahu, Yuna Oh, Gary Peterson, Miguel Cordova, Cristian Navarrete-Dechent, Melissa Gill, Christi Alessi-Fox, Salvador Gonzalez, William Phillips, Steven Wilson, Reza Afzalneia, Raven Rose, Abu-Akeel Mohsere, Danielle Bello, Ashfaq Marghoob, Anthony Rossi, Jedd D. Wolchok, Taha Merghoub, Veronica Rotemberg, Chih-Shan Jason Chen, Milind Rajadhyaksha
Summary: The study explored the feasibility of performing 1-2 mm precision biopsies guided by high-resolution imaging techniques. Results showed that this biopsy method can ensure accurate diagnosis and molecular profiling in the presence of spatial heterogeneity, especially in tumors, providing a new strategy for next-generation histopathology and pathology.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Lixing Zhang, Xin Miao, Meijia Wang, Aihua Shi, Jingwen Wang, Zhonglin Ma, Yunhai Zhang, Jingzhong Zhang, Shuang Yu
Summary: This study reports the development of a portable reflectance confocal microscope (PRCM) for real-time, noninvasive monitoring of skin wound healing. PRCM allows visualization of cellular and nuclear morphology in mouse and human skin, and its results are consistent with histological evaluations. The study provides new insights into monitoring the effects of topically applied mesenchymal stem cells (MSCs) on wound healing using PRCM.
BIO-DESIGN AND MANUFACTURING
(2023)
Article
Medicine, General & Internal
Mihai Lupu, Ana Maria Malciu, Elena Codruta Cozma, Madalina Laura Banciu, Vlad Mihai Voiculescu
Summary: This study aims to evaluate the healthy skin of different age groups, develop an easy-to-use confocal score for quantifying signs of skin aging, and test the correlation between this new score and the clinical score. The results showed a good correlation between the confocal score and clinical score.
Article
Biology
Veronica Rego Moraes, Maisa Oliveira Melo, Patricia M. B. G. Maia Campos
Summary: Diabetes leads to increased production of AGEs, causing irreversible damage to collagen fibers and accelerated signs of skin aging. This study aimed to evaluate diabetic skin's characteristics and compare them to healthy skin.
Review
Oncology
Giuseppe Broggi, Anna Elisa Verzi, Rosario Caltabiano, Giuseppe Micali, Francesco Lacarrubba
Summary: Horizontal histopathology sections and reflectance confocal microscopy images show strong correlation in cutaneous neoplasms, demonstrating the reliability of this innovative, non-invasive technique in managing skin tumors.
FRONTIERS IN ONCOLOGY
(2021)
Article
Optics
Jingxi Li, Jason Garfinkel, Xiaoran Zhang, Di Wu, Yijie Zhang, Kevin de Haan, Hongda Wang, Tairan Liu, Bijie Bai, Yair Rivenson, Gennady Rubinstein, Philip O. Scumpia, Aydogan Ozcan
Summary: The traditional pathological diagnosis of skin tumors involves invasive biopsies and histological staining, which can be cumbersome and result in unnecessary biopsies and scarring. Emerging noninvasive optical technologies, like reflectance confocal microscopy (RCM), offer label-free, cellular-level imaging of skin without the need for a biopsy, but require specialized training due to grayscale images lacking nuclear features.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Biochemical Research Methods
Shicheng Hao, Ying Xiong, Sisi Guo, Jing Gao, Xiaotong Chen, Ruoyu Zhang, Lihui Liu, Jianfeng Wang
Summary: This study reports the development and grading performance validation of a low-cost algorithms-based hyperspectral imaging (aHSI) system for radiodermatitis assessment. The system utilizes Monte Carlo simulations to derive hyper-spectra and physiological parameters of the skin, and achieves high classification accuracy using a one-dimensional convolutional neural network (CNN).
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Urology & Nephrology
Jesus Z. Villarreal, Javiera Perez-Anker, Susana Puig, Marc Xipell, Gerard Espinosa, Esther Barnadas, Ana B. Larque, J. Malvehy, Ricard Cervera, Arturo Pereira, Antonio Martinez-Pozo, Luis F. Quintana, Adriana Garcia-Herrera
Summary: This study demonstrates the feasibility of using ex vivo confocal microscopy for the analysis of basic patterns of lesion in nephropathology. FuCM proves to be as effective as conventional techniques in evaluating acute tubular necrosis and interstitial fibrosis changes, facilitating rapid evaluation of kidney biopsies and restructuring the clinical workflow in renal histopathology.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Biology
Marius Kroger, Jorg Scheffel, Evgeny A. Shirshin, Johannes Schleusener, Martina C. Meinke, Jurgen Lademann, Marcus Maurer, Maxim E. Darvin
Summary: This study demonstrates a noninvasive method (TPE-FLIM) that can visualize macrophages in human dermis. The method shows high sensitivity and specificity, making it valuable for studying the role of macrophages in health and disease.
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Editorial Material
Medicine, General & Internal
Katharine Hanlon, Meredith Thomley, Lilia Correa-Selm
Summary: Demodex folliculorum and Demodex brevis can be detected on facial skin through Reflectance Confocal Microscopy (RCM), appearing as round groupings inside the sebaceous opening. While inflammation may occur, these mites are considered part of normal skin flora. The use of RCM to identify and study these mites may become more common as technology advances.
Review
Oncology
Stefania Guida, Antonio Alma, Kaleci Shaniko, Johanna Chester, Silvana Ciardo, Ilaria Proietti, Roberta Giuffrida, Iris Zalaudek, Marco Manfredini, Caterina Longo, Francesca Farnetani, Giovanni Pellacani
Summary: Non-melanoma skin cancers (NMSC) can be monitored using noninvasive skin imaging in nonsurgical treatments. The accuracy of dermoscopy, reflectance confocal microscopy (RCM), and optical coherence tomography (OCT) in detecting clearance is uncertain. We aim to report the proportion of persisting tumors identified with noninvasive technologies available in the literature.
Article
Pharmacology & Pharmacy
Victor Hugo Pacagnelli Infante, Marcella Gabarra Almeida Leite, Patricia M. B. G. Maia Campos
Summary: This study evaluated the film-forming properties of cosmetic formulations based on starches and Spirulina extract using reflectance confocal microscopy and other instrumental measurements. The formulations demonstrated effective film formation on both skin and hair surfaces, reducing water loss and providing protective effects.
Review
Chemistry, Multidisciplinary
Stefania Guida, Federica Arginelli, Francesca Farnetani, Silvana Ciardo, Laura Bertoni, Marco Manfredini, Nicola Zerbinati, Caterina Longo, Giovanni Pellacani
Summary: Confocal laser scanning microscopy (CLSM) is a valuable tool in clinical settings, providing a quasi-histologic view of tissues without the need for biopsy. It has shown to improve diagnostic accuracy of skin cancers and contribute to reducing unnecessary excisions, as well as improving margin recognition for tissue sparing in excisions. Further studies using CLSM in real-life settings could enhance our understanding of its applications.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Giuseppe Broggi, Anna Elisa Verzi, Francesco Lacarrubba, Giuseppe Micali, Rosario Caltabiano
Summary: Horizontal histopathological sections have shown a strong correlation with images obtained via in vivo reflectance confocal microscopy, providing a useful tool to validate the features of inflammatory disorders. Reflectance confocal microscopy may be a useful diagnostic tool to differentiate diseases with similar clinical presentations.
APPLIED SCIENCES-BASEL
(2022)
Article
Allergy
Mojca Bizjak, Mitja Kosnik, Dejan Dinevski, Simon Francis Thomsen, Daria Fomina, Elena Borzova, Kanokvalai Kulthanan, Raisa Meshkova, Dalia Melina Ahsan, Mona Al-Ahmad, Sabine Altrichter, Andrea Bauer, Maxi Brockstadt, Celia Costa, Semra Demir, Roberta Fachini Criado, Luis Felipe Ensina, Asli Gelincik, Ana Maria Gimenez-Arnau, Margarida Goncalo, Maia Gotua, Jesper Gronlund Holm, Naoko Inomata, Alicja Kasperska-Zajac, Maryam Khoshkhui, Aliya Klyucharova, Emek Kocatuerk, Rongbiao Lu, Michael Makris, Natalya Maltseva, Jovan Miljkovic, Maria Pasali, Marisa Paulino, David Pesque, Jonny Peter, German Dario Ramon, Carla Ritchie, Solange Oliveira Rodrigues Valle, Michael Rudenko, Agnieszka Sikora, Eduardo M. Souza Lima, Nicola Wagner, Paraskevi Xepapadaki, Xiaoyang Xue, Zuotao Zhao, Dorothea Terhorst-Molawi, Marcus Maurer
Summary: Cold urticaria (ColdU) can lead to wheals or angioedema in response to cold exposure, and may be associated with cold-induced anaphylaxis (ColdA). The research found that ColdA can be triggered by a variety of factors, including immersion in cold water and exposure to cold air. Patients with concomitant chronic spontaneous urticaria have a lower risk of ColdA in ColdU.
Article
Allergy
Margitta Worm, Aikaterina Alexiou, Andrea Bauer, Regina Treudler, Gerda Wurpts, Heinrich Dickel, Timo Buhl, Sabine Mueller, Andreas Jung, Randolf Brehler, Joachim Fluhr, Ludger Klimek, Norbert Muelleneisen, Wolfgang Pfuetzner, Ulrike Raap, Stefani Roeseler, Sandra Schuh, Hartmut Timmermann, Guido Heine, Bettina Wedi, Knut Brockow
Summary: In this study, the management and outcome of suspected SARS-CoV-2 vaccine hypersensitivity were assessed. It was found that proven IgE-mediated hypersensitivity to SARS-CoV-2 vaccines is extremely rare and not increased in comparison with reported hypersensitivity to other vaccines. The value of skin tests is unclear and nonspecific reactions should be considered.
Article
Dermatology
Joachim W. Fluhr, Peter Menzel, Rolf Schwarzer, Benjamin Kaestle, Michaela Arens-Corell, Lina Praefke, Nikolai K. Tsankov, Dessyslava G. Nikolaeva, Laurent Misery, Razvigor Darlenski
Summary: This study investigated the effects of different cosmetic lotions on skin microbiome and skin physiology after mild tape stripping. The results showed that the lotions accelerated the recovery of the microbiome and increased stratum corneum hydration. The composition of the lotion and induced repair mechanism may be responsible for the accelerated normalization of the microbiome.
SKIN PHARMACOLOGY AND PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Joachim W. Fluhr, Ali Tfayli, Razvigor Darlenski, Maxim E. Darvin, Nicolas Joly-Tonetti, Nadege Lachmann
Summary: This study aimed to establish an in vitro SC model to evaluate differences in the efficacy of a natural sugar-derived complex in combination with glycerol and a botanical extract in modulating SC water binding and structural proteins and barrier lipids.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Allergy
Dorothea Terhorst-Molawi, Tomasz Hawro, Eva Grekowitz, Lea Kiefer, Kunal Merchant, Diego Alvarado, Lawrence J. Thomas, Thomas Hawthorne, Elizabeth Crowley, Margo Heath-Chiozzi, Martin Metz, Marcus Maurer
Summary: In this study, Barzolvolimab showed good tolerability and demonstrated significant reduction in skin mast cells and circulating tryptase levels in patients with chronic inducible urticaria (CIndU). It also improved disease control and quality of life in clinical settings.
Editorial Material
Dermatology
Torsten Zuberbier, Sabine Altrichter, Sabine Bauer, Randolf Brehler, Knut Brockow, Corinna Dressler, Joachim Fluhr, Matthew Gaskins, Eckard Hamelmann, Kathrin Kuehne, Hans Merk, Norbert K. Muelleneisen, Alexander Nast, Heidi Olze, Hagen Ott, Marc Pleimes, Franziska Rueff, Petra Staubach-Renz, Bettina Wedi, Marcus Maurer
Summary: This publication is the first section of the Urticaria guideline, which covers the classification and diagnosis of urticaria, as well as the causes, triggering factors, and pathomechanisms. It also addresses strategies for efficient diagnosis of different subtypes of urticaria, providing important guidance for individualized, patient-centered treatment.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Editorial Material
Dermatology
Torsten Zuberbier, Sabine Altrichter, Sabine Bauer, Randolf Brehler, Knut Brockow, Corinna Dressler, Joachim Fluhr, Matthew Gaskins, Eckard Hamelmann, Kathrin Kuehne, Hans Merk, Norbert K. Muelleneisen, Alexander Nast, Heidi Olze, Hagen Ott, Marc Pleimes, Franziska Rueff, Petra Staubach-Renz, Bettina Wedi, Marcus Maurer
Summary: This article is the first section of the Urticaria Guideline, focusing on the classification and diagnosis of urticaria, as well as strategies for efficient diagnosis. Urticaria is a severe allergic disease with a lifetime prevalence of almost 20%, which significantly impacts the quality of life and performance of those affected.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Dermatology
Torsten Zuberbier, Sabine Altrichter, Sabine Bauer, Randolf Brehler, Knut Brockow, Corinna Dressler, Joachim Fluhr, Matthew Gaskins, Eckard Hamelmann, Kathrin Kuehne, Hans Merk, Norbert K. Muelleneisen, Alexander Nast, Heidi Olze, Hagen Ott, Marc Pleimes, Franziska Rueff, Petra Staubach-Renz, Bettina Wedi, Marcus Maurer
Summary: This publication is the second part of the German-language S3 guideline on urticaria, focusing on the management of the condition. It highlights the importance of symptomatic treatment when causal factors cannot be eliminated. The recommended first-line treatment is non-sedating H1 antihistamines.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Review
Chemistry, Applied
Razvigor Darlenski, Joachim W. Fluhr
Summary: The skin's multiple protective functions rely on the interactions between epithelial skin, immune cells, and the commensal microbiota. After birth, the skin barrier undergoes dynamic adaptation, with specific differences in structure and physiology compared to adult skin. These adaptations include thinner stratum corneum, increased protease activity, and accelerated proliferation in infant skin. The colonization of commensal bacteria immediately after birth plays a crucial role in the maturation of the immune system. This paper focuses on the ongoing adaptation of the human skin barrier after birth and the interactions between the skin barrier, microbiota, and immune system.
INTERNATIONAL JOURNAL OF COSMETIC SCIENCE
(2023)
Review
Dermatology
Dorothea Terhorst-Molawi, Lena Fox, Frank Siebenhaar, Martin Metz, Marcus Maurer
Summary: The goal of treating chronic spontaneous urticaria is to effectively and safely eliminate the disease. Current treatment options target mast cell mediators or activators. Treatment should continue until complete control of the disease and normalization of quality of life.
AMERICAN JOURNAL OF CLINICAL DERMATOLOGY
(2023)
Article
Dermatology
Joachim W. Fluhr, Georg Wiora, Dessyslava G. Nikolaeva, Laurent Misery, Razvigor Darlenski
Summary: This study aimed to compare the new multi-sensor probe (Tewameter TM Hex) with the established Tewameter TM 300 probe and obtain reference data for transepidermal energy loss and water vapor concentration on the skin. The results showed a significant correlation between TM Hex and TM 300, with TM Hex providing more accurate measurements.
SKIN RESEARCH AND TECHNOLOGY
(2023)
Review
Allergy
Sherezade Monino-Romero, Yana Hackler, Tiia-Linda Okas, Eva Maria Grekowitz, Joachim W. Fluhr, Vincent Hultsch, Lea Alice Kiefer, Polina Pyatilova, Dorothea Terhorst-Molawi, Yi-Kui Xiang, Frank Siebenhaar, Marcus Maurer, Pavel Kolkhir
Summary: Chronic spontaneous urticaria (CSU) is believed to have an autoimmune component in at least 8% of patients, characterized by mast cell-activating IgG autoantibodies. Basophil tests such as BAT and BHRA are considered the best single tests for diagnosing autoimmune CSU. However, the relationship between positive basophil tests and CSU characteristics, patient demographics, and treatment response remains poorly understood.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Allergy
Ulrike Foerster-Ruhrmann, Dafni Stergioudi, Agnieszka J. Szczepek, Joachim W. Fluhr, Torsten Zuberbier, Heidi Olze, Karl-Christian Bergmann
Summary: This retrospective study compared the clinical efficacy of mepolizumab, benralizumab, omalizumab, and dupilumab in patients with severe asthma and comorbid chronic rhinosinusitis with nasal polyps. The results showed that dupilumab had the most significant improvement in asthma and nasal symptoms. Overall, T2-targeting biologics effectively treated severe asthma with comorbid chronic rhinosinusitis with nasal polyps, but the efficacy varied among different biologics in treating chronic rhinosinusitis with nasal polyps.
WORLD ALLERGY ORGANIZATION JOURNAL
(2023)
Article
Allergy
Sabine Altrichter, Emilia Mellerowicz, Dorothea Terhorst-Molawi, Eva Grekowitz, Karsten Weller, Marcus Maurer
Summary: Patients with CholU have many unmet medical needs, and there is a need for improvement in the diagnostic workup and patient care, as well as better treatment options.
FRONTIERS IN ALLERGY
(2022)
