Article
Materials Science, Multidisciplinary
A. Ramos-Valle, L. Marin-Caba, L. Garcia Hevia, M. A. Correa-Duarte, M. L. Fanarraga
Summary: Repairing genetic defects using exogenous DNA is a major challenge, and the design of effective vectors for encapsulating and delivering DNA to specific cells is crucial. The silica-based particles developed in this study provide stable encapsulation and protection of DNA, even under various stress conditions. The versatility of the design allows for the sequential expression of multiple genes, mimicking viral gene expression cascades, and the method is scalable, reproducible, and cost-effective, making it valuable for the personalized therapeutics industry.
MATERIALS TODAY ADVANCES
(2023)
Article
Multidisciplinary Sciences
Juan C. Fraire, Elnaz Shaabani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens, Dawei Hua, Jana Ramon, Laurens Raes, Eduardo Bolea-Fernandez, Toon Brans, Frank Vanhaecke, Peter Borghgraef, Chaobo Huang, Felix Sauvage, Tamara Vanhaecke, Joery De Kock, Ranhua Xiong, Stefaan De Smedt, Kevin Braeckmans
Summary: Biolistic delivery with light-triggered self-assembled nanobombs can efficiently transfect mammalian cells by propelling smaller nanoprojectiles through the cell membrane using vapor bubble formation. It outperforms electroporation in terms of transfection yield.
NATURE COMMUNICATIONS
(2022)
Article
Materials Science, Biomaterials
Haonan Huo, Xingdi Cheng, Jiaxi Xu, Jiaqi Lin, Ning Chen, Xueguang Lu
Summary: In this study, a new ionizable lipid containing fluorinated alkyl chains (F-L319) was synthesized and compared with its hydrocarbon counterpart (L319) for mRNA delivery efficiency. It was found that combining the appropriate ratios of F-L319 and L319 as hybrid ionizable lipids in LNPs greatly enhanced mRNA delivery efficiency both in vitro and in vivo.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biotechnology & Applied Microbiology
Federica Ponti, Nina Bono, Luca Russo, Paolo Bigini, Diego Mantovani, Gabriele Candiani
Summary: In this study, a new technology to enhance the intracellular delivery of nucleic acids and improve the transfection efficiency of non-viral vectors through priming adherent cells with a short vibrational stimulation is focused on.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Nasha Qiu, Zhen Zhang, Xuyong Wei, Chang Xu, Xiaolong Jia, Kai Wang, Yunqi Chen, Shuai Wang, Renyi Su, Beini Cen, Youqing Shen, Chunying Chen, Yanpeng Liu, Xiao Xu
Summary: Peritoneal metastasis is common in late-stage or postsurgery gastrointestinal, reproductive, and genitourinary tract cancers, leading to poor prognosis. Therefore, finding effective and nontoxic prophylactic strategies against peritoneal metastasis is crucial. This study demonstrates the first gene transfection as a nontoxic prophylaxis for preventing peritoneal metastasis or operative metastatic dissemination. TRAIL-transfected lipopolyplexes enable long-term expression of TRAIL in the peritoneum and macrophages, selectively inducing tumor cell apoptosis while sparing normal tissue, effectively preventing tumor nodules formation and prolonging mouse survival time compared to chemotherapy prophylaxis.
Article
Multidisciplinary Sciences
Yue Wu, Afu Fu, Gilad Yossifon
Summary: In this study, a metallodielectric hybrid micromotor was used to perform localized electroporation and gene transfection of mammalian cells, showing high reversible electroporation yield and precise targeting of cells. The micromotor was able to enhance gene introduction into difficult-to-transfect cells and demonstrated successful gene expression post transfection, which is significant for molecular biology research.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Jennifer Lien, Tawni Bull, Richard W. Michelmore, Ting Guo
Summary: Fluorescence titration using magnetic nanoparticles (FTMN) is a rapid, inexpensive, and simple method for quantifying the amount of fluorophore-intercalated plasmid DNA on DNA attractive nanoparticles. By confirming the binding of propidium iodide (PI)-intercalated DNA to polyethylenimine (PEI)-coated monodisperse iron oxide magnetic nanoparticles (PEI-MNs), the FTMN method provides a way to determine the amount of DNA on MNs, the dissociation constant, and binding energy. This method is validated by utilizing quantitative polymerase chain reaction to measure the amount of free PI/DNA in solution.
ANALYTICAL CHEMISTRY
(2021)
Article
Optics
Stijn Cuyvers, Bahawal Haq, Camiel Op de Beeck, Stijn Poelman, Artur Hermans, Zheng Wang, Agnieszka Gocalinska, Emanuele Pelucchi, Brian Corbett, Gunther Roelkens, Kasper Van Gasse, Bart Kuyken
Summary: Research shows that leveraging ultra-low losses of silicon-nitride waveguides to manufacture a III-V-on-silicon-nitride integrated optical comb laser leads to superior performance, high efficiency, and low manufacturing costs.
LASER & PHOTONICS REVIEWS
(2021)
Article
Nanoscience & Nanotechnology
Ian Bauwens, Krishan Harkhoe, Peter Bienstman, Guy Verschaffelt, Guy Van der Sande
Summary: This study proposes using transfer learning to address the issue of parameter drift in photonic reservoir computing system and reduce the resources required for retraining. Numerical studies on a delay-based system with semiconductor lasers demonstrate that transfer learning can mitigate parameter fluctuations and reduce training requirements for the second task.
Article
Pharmacology & Pharmacy
Michael Karimov, Dietmar Appelhans, Alexander Ewe, Achim Aigner
Summary: This study explores the combination of cross-linking small PEI molecules with biodegradable disulfide-groups and modifying them with tyrosine to enhance DNA delivery efficiency. The synergistic effects of these modifications result in improved transfection efficacy and biocompatibility, making them promising for therapeutic applications.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Optics
Patrycja Spiewak, Robert P. Sarzala, Michal Wasiak
Summary: In this study, the impact of cavity Q factor and the number of quantum wells on the small-signal modulation response of GaAs-based VCSELs was analysed. By adjusting the mirror's reflectivity, it is possible to achieve flat response curves with a high -3 dB bandwidth. Increasing the number of quantum wells in the active region is also beneficial.
OPTICS AND LASER TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Angela M. Kemfack, Ignacio Hernandez-Morato, Yalda Moayedi, Michael J. Pitman
Summary: This study developed a method to extract high-quality RNA from intrinsic laryngeal muscles and validated its application in RNA sequencing. The method showed good reproducibility and broad applicability, providing valuable insights for therapeutic research on voice, swallowing, and airway disorders.
SCIENTIFIC REPORTS
(2022)
Review
Biochemical Research Methods
Sepanta Hosseinpour, Laurence J. Walsh
Summary: Gene therapy has shown effectiveness in treating certain conditions, and laser therapy has the potential to enhance nucleic acid transfection through photomechanical, photothermal, and photochemical means. Different approaches such as optoporation, photomechanical gene transfection, and photochemical internalization have been explored, with varying levels of effectiveness for in vitro and in vivo applications. Additional research is needed to optimize laser protocols for improving gene therapy outcomes.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Pharmacology & Pharmacy
Hiroshi Kida, Loreto B. Feril, Yutaka Irie, Hitomi Endo, Keiji Itaka, Katsuro Tachibana
Summary: This study examines the effect of different sizes of bubbles on gene transfer efficiency in ultrasound-mediated gene therapy. The results show that bubbles larger than 200 nm have a greater contribution to delivering genes into the cytoplasm using ultrasound, offering valuable information for optimizing nanobubbles in future gene therapy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Physical
Montacer Dridi, Amine Jaouadi, Florent Colas, Chantal Compere
Summary: This paper investigates the lasing action in strongly coupled plasmonic nanocavity arrays, showing that the lasing action is closely related to near-field enhancement and depends on pumping energy. The enhancement mechanism is mainly based on energy transfer from molecules composing the gain medium to the plasmonic mode at the nanoscale.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)