Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 6, Pages -Publisher
SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3526357
Keywords
biophotonic; Raman spectroscopy; biology; biomedical optics
Funding
- Guangxi Science and Technology Department of China [0895001-1-1]
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We report on development of dual-trap Raman tweezers for monitoring cellular dynamics and heterogeneity of interacting living cells suspended in a liquid medium. Dual-beam optical tweezers were combined with Raman spectroscopy, which allows capturing two cells that are in direct contact or closely separated by a few micrometers and simultaneously acquiring their Raman spectra with an imaging CCD spectrograph. As a demonstration, we recorded time-lapse Raman spectra of budding yeast cells held in dual traps for over 40 min to monitor the dynamic growth in a nutrient medium. We also monitored two germinating Bacillus spores after the initiation with L-alanine and observed their heterogeneity in the release of CaDPA under identical microenvironment. (C) 2010 Society of Photo-Optical instrumentation Engineers. [DOI: 10.1117/1.3526357]
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