Article
Pharmacology & Pharmacy
Huu Van Le, Virginie Dulong, Luc Picton, Didier Le Cerf
Summary: The lyophilization of nanogels allows for long-term conservation and adjustment of their concentration and dispersant type. This study investigated the effects of formulation aspects on the integrity of polyelectrolyte complex nanogels after lyophilization and reconstitution. The best approach for freeze-drying thermoresponsive PEC-NGs was determined, and it was found that freeze-drying PEC-NG suspensions prepared at a relatively low polymer concentration with trehalose as a cryoprotectant allowed for homogeneous redispersion of PEC-NGs without significant aggregation.
Article
Chemistry, Applied
Zhixiang Cui, Lu Qin, Shuang Guo, Hongbo Cheng, Xin Zhang, Jian Guan, Shirui Mao
Summary: A nanocomplex system targeting the intestines was developed using biotin modification and hyaluronic acid coating to enhance penetration efficiency and achieve significant hypoglycemic effects.
CARBOHYDRATE POLYMERS
(2021)
Review
Chemistry, Multidisciplinary
Huu Van Le, Didier Le Cerf
Summary: Hyaluronic acid-based colloidal polyelectrolyte complexes (PECs), due to their high biocompatibility and simple preparation process, have a wide range of applications in the biomedical field. This review aims to provide a comprehensive understanding of the preparation process and various biomedical applications of colloidal PECs from hyaluronic acid complexed with polycations.
Article
Nanoscience & Nanotechnology
Haw-Ming Huang, Ping-Han Wu, Po-Chien Chou, Wen-Tien Hsiao, Hsin-Ta Wang, Hsin-Pei Chiang, Chi-Ming Lee, Shwu-Huey Wang, Yu-Cheng Hsiao
Summary: The study shows that LMWHA-IONPs can effectively enhance MRI contrast for the early diagnosis of glioblastoma, with good targeting ability and biocompatibility towards tumor cells.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Josefa Domenech, Mariana de Britto, Antonia Velazquez, Susana Pastor, Alba Hernandez, Ricard Marcos, Constanza Cortes
Summary: The study assessed the long-term effects of polystyrene nanoparticles on human intestinal cells, showing that PSNPs accumulated in cells over time and induced changes at the ultrastructural and molecular levels. However, minor effects were observed in genotoxicity-related biomarkers, indicating no DNA damage or oxidative stress after long-term exposure to PSNPs.
Article
Engineering, Biomedical
Mario C. Fonseca, Ana Catarina Vale, Rui R. Costa, Rui L. Reis, Natalia M. Alves
Summary: Inspired by mussel bioadhesion, researchers developed adhesive membranes using catechol-modified polysaccharides and a simple compaction method. These membranes exhibited strong adhesive properties and could also incorporate bioactive glass nanoparticles to stimulate mineralization, making them potentially suitable for hard tissue regeneration.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Instruments & Instrumentation
Shaymaa S. S. Seoudi, Eman A. A. Allam, Amal H. H. El-Kamel, Hagar Elkafrawy, Riham M. M. El-Moslemany
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory disease along the gastrointestinal tract that requires effective treatment with targeted drug delivery. This study presents pH-responsive hyaluronic acid (HA) coated Eudragit S100 nanoparticles (HA-BUD-ES-NPs) for the targeted delivery of budesonide (BUD). HA-BUD-ES-NPs showed good stability, high entrapment efficiency, and pH-dependent release. In vitro and in vivo studies demonstrated the biocompatibility, enhanced cellular uptake, anti-inflammatory effects, and improved therapeutic efficacy of HA-BUD-ES-NPs in an IBD model. The findings suggest that HA-BUD-ES-NPs provide a promising drug delivery platform for the management and site-specific treatment of IBD.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Biophysics
Ji Wang, Wei He, Tao Wang, Man Li, Xinsong Li
Summary: By adding 30 mg/L SM-Fe NPs, significant improvement in the production of hyaluronic acid (HA) was observed, with the dry weight of HA produced increasing by 3.28 times compared to the control group, presenting promising opportunities for industrial production.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Medicinal
Sarah Akhlaghi, Shahram Rabbani, Hanieh Karimi, Azadeh Haeri
Summary: This study evaluated the preventive effect of curcumin-soybean phosphatidylcholine (CUR-SPC) complex-loaded nanoparticles (NPs) embedded in hyaluronic acid (HA) gel on peritoneal adhesions following abdominal procedures. The optimized CUR-SPC NPs showed a high CUR loading and nanometric particle size. The CUR-SPC-HA gel exhibited a sustained release profile and inhibited fibroblast proliferation and collagen synthesis.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Xiyang Sun, Ye Xu, Qingkui Guo, Ning Wang, Bin Wu, Chen Zhu, Wen Zhao, Wenliang Qiang, Min Zheng
Summary: This study successfully constructed a targeted nanoprobe that can efficiently load the photosensitive drug ICG into lung cancer cells. The nanoprobe not only has excellent ICG loading and stability, but also improves the efficiency of thermal conversion and singlet oxygen generation, resulting in optimal photothermal/photodynamic therapy effects.
Article
Pharmacology & Pharmacy
Saoirse Casey-Power, Camila Vardar, Richie Ryan, Gautam Behl, Peter McLoughlin, Mark E. Byrne, Laurence Fitzhenry
Summary: This study presents a novel NAD+-associated polymeric nanoparticle system, formulated through the complexation of natural polyelectrolytes hyaluronic acid and poly(L-lysine) (PEC). The PEC exhibited suitable physicochemical properties for ocular drug delivery, including appropriate particle size and sustained release at physiological ocular flow rates. Moreover, the PEC showed no significant cytotoxic effects on corneal epithelial cells and demonstrated retention within the corneal epithelium layer. The study highlights the potential of the PECs as a promising platform for improving ocular health.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Yue Sui, Yue Gu, Yujing Lu, Chenxu Yu, Jie Zheng, Hang Qi
Summary: By encapsulating FX in PVP-coated nanoparticles, the study improved FX stability and delivery into cancer cells, showing pro-oxidative activities. This approach could potentially be a new therapeutic strategy for targeted cancer treatment.
Article
Chemistry, Applied
Huu Van Le, Virginie Dulong, Luc Picton, Didier Le Cerf
Summary: A novel kind of thermoresponsive polyelectrolyte complex-based nanogels (PEC-NGs) were developed by mixing hyaluronic acid functionalized with Jeffamine M-2005 and either DEAE-D or PLL. The presence of M2005 grafts led to PEC-NGs with larger particle size, lower net surface charge, and thermoresponsiveness. Replacing DEAE-D with PLL improved stability against salinity and encapsulation of curcumin.
CARBOHYDRATE POLYMERS
(2022)
Article
Biotechnology & Applied Microbiology
Dilip Panwar, Pradip Thakor, Madhu Sharma, Avijit Kumar Bakshi, Valamla Bhavana, Vaibhavi Srivastava, Prabhat Ranjan Mishra, Shashi Bala Singh, Neelesh Kumar Mehra
Summary: The aim of this study was to target breast cancer using hyaluronic acid-coated venetoclax nanocrystals (HA-VEN-NCs) by repurposing venetoclax. The nanocrystals were fabricated using an antisolvent precipitation method and optimized using central composite design. Hyaluronic acid (HA)-coated and -uncoated nanocrystals were compared in terms of drug release, cell line studies, CD44-expressing breast tumor cell binding capability, and anticancer activity. The findings showed that HA-VEN-NCs efficiently bind to CD44-expressing breast tumor cells and exhibit excellent anticancer activity, indicating their potential as valuable nanoplatforms for cancer treatments.
Article
Biochemistry & Molecular Biology
Gi-Beum Kim, Kwansung Seo, Jong-Ung Youn, Il Keun Kwon, Jinny Park, Kwang-Hyun Park, Jong-Suk Kim
Summary: In this study, the researchers developed natural and functional materials with antioxidant and anti-inflammatory effects. They extracted extracts from natural plants and prepared an extract composite called effective unsaturated fatty acid complex (EUFOC). The EUFOC showed excellent antioxidant and anti-inflammatory properties, inhibiting nitric oxide production and increasing hyaluronic acid content. These results indicate that EUFOC can be used as a functional material in various fields.
Article
Pharmacology & Pharmacy
Daniel Kramarczyk, Justyna Knapik-Kowalczuk, Wojciech Smolka, Maria Ferreira Monteiro, Lidia Tajber, Marian Paluch
Summary: This article investigates the effect of mesoporous silica on the physical stability and molecular dynamics of the amorphous form of Celecoxib. It has been found that mesoporous silica can slow down the recrystallization process of Celecoxib and the formation of a monomolecular drug layer on the silica's surface is associated with the observed inhibition of recrystallization.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Martin McHugh, Sarah Hayes, Lidia Tajber, Laurie Ryan
Summary: Public engagement in medicine plays a crucial role in promoting population health management and literacy. However, current activities often have a narrow focus, only targeting specific patient groups, diseases, or drugs. Therefore, a broader approach is needed to improve the overall health literacy of the general public.
JOURNAL OF CHEMICAL EDUCATION
(2022)
Article
Chemistry, Multidisciplinary
Mariana Hugo Silva, Sarah P. Hudson, Lidia Tajber, Matthieu Garin, Wenyu Dong, Tatsiana Khamiakova, Rene Holm
Summary: This study investigated the difference in methods to determine the osmolality in solutions of stabilizers used for long-acting injectable suspensions. The results showed that the osmolality increased with increasing concentration for all polymers and surfactants, regardless of the measuring method used. Different measurement methods yielded different osmolality values for PVPs, PEGs, and Na-CMC. The vapor pressure method may be more suitable when the formulations contain higher concentrations of excipients and/or thermosensitive excipients.
PHARMACEUTICAL RESEARCH
(2023)
Editorial Material
Chemistry, Multidisciplinary
Lynne S. Taylor, Doris E. Braun, Lidia Tajber, Jonathan W. Steed
CRYSTAL GROWTH & DESIGN
(2022)
Article
Medicine, Research & Experimental
Julija Zotova, Brendan Twamley, Lidia Tajber
Summary: Acid-base multicomponent systems are commonly used to fine-tune the physicochemical properties of active pharmaceutical ingredients. However, the nature of intermolecular interactions within these systems is not accurately predicted by current prediction tools. In this study, the effect of carbon backbone length and flexibility on the molecular recognition, crystallization, and thermal behavior of lidocaine (LID) and dicarboxylic acid mixtures was investigated. The formation of eutectic phases, hydrogen bonding between the reacting components, and possible formation of new phases stemming from partial ionization of the counterions were observed.
MOLECULAR PHARMACEUTICS
(2022)
Editorial Material
Medicine, Research & Experimental
Lynne S. Taylor, Doris E. Braun, Lidia Tajber, Jonathan W. Steed
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Mariana Hugo Silva, Ajay Kumar, Benjamin K. Hodnett, Lidia Tajber, Rene Holm, Sarah P. Hudson
Summary: This study describes a novel approach combining liquid antisolvent precipitation with seeding to obtain a stable form of indomethacin API and achieve the desired particle size distribution. The results show that excipients and seeds influence the rate of transformation of indomethacin, and the particle size distribution and timing of seed addition are critical for obtaining the desired solid-state form.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Nanoscience & Nanotechnology
Justyna Hajtuch, Eliza Iwicka, Anna Szczoczarz, Damian Flis, Elzbieta Megiel, Piotr Cieciorski, Marek Witold Radomski, Maria Jose Santos-Martinez, Iwona Inkielewicz-Stepniak
Summary: In this study, we synthesized eptifibatide-functionalized silver nanoparticles and evaluated their pharmacological activity on platelets and endothelial cells. The results showed that AgNPs-EPI exhibited anti-aggregatory activity by regulating platelet aggregation, blood coagulation, and endothelial cell activity, at concentrations lower than the free drug. These findings provide preliminary evidence that AgNPs can be used as an effective delivery platform for antiplatelet drugs.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Pharmacology & Pharmacy
Viviane Lucia Beraldo-Araujo, Ana Flavia Siqueira Vicente, Marcelo van Vliet Lima, Anita Umerska, Eliana B. Souto, Lidia Tajber, Laura Oliveira-Nascimento
Summary: Preformulation studies play a crucial role in the successful development of nanoformulations, as they help identify the best excipients and understand drug-excipient compatibility and interactions. This study focused on the development of nanostructured lipid carriers (NLCs) for delivering the antibiotic levofloxacin (LV) and systematically evaluated the critical process parameters to establish a stable system with high drug entrapment efficiency (EE).
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Physical
Snehashis Nandi, Luis Padrela, Lidia Tajber, Alain Collas
Summary: The fundamental understanding of solubility and nucleation kinetics is crucial for the development of liquid antisolvent crystallization (LASC) process. This study determined the solubility of intermediate A in different solvents and binary mixtures using a liquid chromatographic method. The selection of the optimum scale and mixing conditions for measuring the metastable zone width (MSZW) in nucleation kinetics was demonstrated to be important. The derived kinetic parameters and accurate solubility data can guide solvent selection for the generation of nanosuspensions in LASC.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Multidisciplinary
Fidel Mendez Canellas, Vivek Verma, Jacek Kujawski, Robert Geertman, Lidia Tajber, Luis Padrela
Summary: The effects of processing conditions on the polymorphism of indomethacin were studied, and it was found that consistent formation of the pure alpha polymorphic form was only achieved when poloxamer 407 was used as an additive. The GAS method combined with poloxamer 407 as a molecular additive allowed for full control over the polymorphic form of indomethacin, regardless of the processing conditions employed.
Article
Pharmacology & Pharmacy
Snehashis Nandi, Luis Padrela, Lidia Tajber, Alain Collas
Summary: This study demonstrates the operational feasibility of generating and stabilizing long-acting injectable suspensions of a practically insoluble drug, itraconazole, using continuous liquid antisolvent crystallization and downstream processing. The optimized microchannel reactor-based bottom-up crystallization setup and selection of suitable solvent and stabilizer resulted in a stable suspension with desired particle size distribution and solid concentration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Fidel Mendez Canellas, Robert Geertman, Lidia Tajber, Luis Padrela
Summary: Co-spraying of APIs with excipients using supercritical CO2-assisted spray drying (SASD) was investigated to address formulation challenges. Results showed that SASD yielded particles with sizes ranging from 0.4-7.6 μm, where naproxen crystalline Form 1 particles were embedded in an amorphous sorbitol matrix, while indomethacin-sorbitol mixtures exhibited partial crystallinity. Naproxen-sorbitol mixtures formed homogeneous microparticle suspensions upon contact with water, while indomethacin-sorbitol mixtures were not reconstitutable. The API-sorbitol interactions were studied in detail using thermal analysis and COSMO-RS modelling. Overall, this work provides a better understanding of co-spraying of APIs with excipients for the formulation of reconstitutable dried microparticles.
JOURNAL OF SUPERCRITICAL FLUIDS
(2023)
Article
Chemistry, Medicinal
Magdalena Paczkowska-Walendowska, Lidia Tajber, Andrzej Miklaszewski, Judyta Cielecka-Piontek
Summary: This study prepared solid dispersions containing polydatin-rich Polygoni cuspidati extract by hot melt extrusion, aiming to enhance the release of poorly water-soluble compounds. The formulation based on Kollidon (R) VA64 exhibited the best tabletability properties and mucoadhesive properties.
Article
Medicine, Research & Experimental
Julija Zotova, Brendan Twamley, Lidia Tajber
Summary: Multicomponent systems consisting of acids and bases are commonly used to fine-tune the properties of pharmaceutical ingredients. However, current prediction tools are not always accurate in predicting the interactions within these systems. In this study, the authors investigated the effects of acid carbon backbone length and flexibility on the behavior of mixtures containing lidocaine and dicarboxylic acids. The results showed the formation of new phases and deviations from predicted eutectic melting points, indicating the complex nature of these systems. Additionally, the structure of a previously unknown form of tridecanedioic acid was determined.
MOLECULAR PHARMACEUTICS
(2022)
