Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 7, Issue 4, Pages 535-541Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2011.1321
Keywords
Immunomagnetic Reduction; Vascular Endothelial Growth Factor; Magnetic Nanoparticle; Hepatocellular Carcinoma; Animal Model
Funding
- National Science Council of Taiwan [98-2112-M-003-003, 98-2323-B-003-001-CC2, NSC98-2752-M-002-016-PAE]
- Department of Health [DOH98-TD-N-111-008, DOH99-TD-N-111-008]
- Ministry of Economic Affairs of Taiwan [1Z970688, 1Z0990415(SBIR), S09800226-203 (JAID)]
Ask authors/readers for more resources
To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available