Article
Engineering, Biomedical
Teng Ma, Yiming Hao, Shengyou Li, Bing Xia, Xue Gao, Yi Zheng, Liangwei Mei, Yitao Wei, Changbin Yang, Lei Lu, Zhuojing Luo, Jinghui Huang
Summary: In this study, a sequential oxygen supply system was developed using core-shell fibers to repair peripheral nerve defects. The system showed significant improvements in cell protection, angiogenesis, and nerve regeneration. The sequential oxygen supply conduits acted as oxygen suppliers for cells and resulted in similar functional outcomes as autografts.
Article
Engineering, Biomedical
Xianhao Dong, Siyang Liu, Yueyue Yang, Shan Gao, Wenlei Li, Jiasong Cao, Ye Wan, Ziqi Huang, Guanwei Fan, Quan Chen, Hongjun Wang, Meifeng Zhu, Deling Kong
Summary: Research on nerve conduits composed of oriented microfiber-bundle cores and randomly organized nanofiber sheaths has shed light on the regulatory role of microfiber orientation in promoting peripheral nerve regeneration, elucidating a cascade of biological responses and ultimately enhancing nerve regeneration outcomes.
Article
Biochemistry & Molecular Biology
Yan Kong, Jiawei Xu, Qi Han, Tiantian Zheng, Linliang Wu, Guicai Li, Yumin Yang
Summary: In this study, a pure porcine decellularized nerve matrix conduit (xDNME) was successfully developed using an electrospinning technique without any additives. The xDNME conduit exhibited an obvious fiber structure and retained the natural components of the decellularized nerve tissue. In vivo experiments showed that xDNME significantly promoted the regeneration of rat sciatic nerve and exhibited comparable myelin sheath thickness and electrical signal transmission ability to the autologous nerve.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Multidisciplinary
Renliang Zhao, Lianghua Jiang, Juan Du, Bitong Xu, Ang Li, Wei Wang, Shichang Zhao, Xiaolin Li
Summary: The biomimetic electrospun conduit Gel@PLGA/Col showed outstanding nerve regeneration performance in a sciatic nerve defect implantation model, inducing axon growth and facilitating nerve continuity recovery. This structure holds great potential for peripheral nerve repair and functional recovery after injury.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Polymer Science
Luca Cicero, Roberto Puleio, Giovanni Cassata, Roberta Cirincione, Lawrence Camarda, Dario Caracappa, Lorenzo D'Itri, Mariano Licciardi, Giulio Edoardo Vigni
Summary: In this study, a microfibrous tubular scaffold made of biocompatible and biodegradable poly(1,4-butylene succinate) (PBS) was designed and fabricated through electrospinning. These scaffolds were evaluated as nerve guide conduits in a rat model of sciatic nerve neurotmesis, demonstrating their biodegradability and efficacy in enhancing the reconstruction process over a long-term period (1-year follow-up). Histological assay and electrophysiological evaluation were performed to compare the long-term outcomes following sutureless repair with the microfibrillar wrap to outcomes obtained using traditional suture repair.
Article
Engineering, Biomedical
Melissa L. D. Rayner, Adam G. E. Day, Kulraj S. Bhangra, John Sinden, James B. Phillips
Summary: This study provides evidence supporting the effectiveness of using engineered neural tissue (EngNT) as a replacement for nerve autografts, with functional regeneration assessed through histological and electrophysiological outcome measures demonstrating equivalent performance.
ACTA BIOMATERIALIA
(2021)
Review
Cell Biology
Sara Saffari, Tiam M. Saffari, Dietmar J. O. Ulrich, Steven E. R. Hovius, Alexander Y. Shin
Summary: The microenvironment at the site of injury plays a crucial role in altering the degree of nerve regeneration after peripheral nerve injury. Stem cells and vascularity are proposed to be important factors in enhancing peripheral nerve regeneration, with stem cells secreting various angiogenic factors and regulating angiogenesis to promote nerve regeneration and vascular expansion.
NEURAL REGENERATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ema Onode, Takuya Uemura, Kiyohito Takamatsu, Takuya Yokoi, Kosuke Shintani, Shunpei Hama, Yusuke Miyashima, Mitsuhiro Okada, Hiroaki Nakamura
Summary: This study found that nerve conduits coated with human induced pluripotent stem cell (hiPSC)-derived neurospheres can promote nerve regeneration and functional recovery in repairing rat sciatic nerve defects. This nerve regeneration approach shows promise as a new clinical therapy for treating peripheral nerve defects.
SCIENTIFIC REPORTS
(2021)
Review
Chemistry, Multidisciplinary
Kai Liu, Lesan Yan, Ruotao Li, Zhiming Song, Jianxun Ding, Bin Liu, Xuesi Chen
Summary: Nerve autograft is currently the preferred treatment for peripheral nerve defects but has limitations. Emerging nerve guide conduits (NGCs), especially those made through 3D printing, offer effective platforms for repairing peripheral nerve defects.
Article
Neurosciences
Elsa Gonzalez-Cubero, Maria Luisa Gonzalez-Fernandez, Maria Rodriguez-Diaz, Marta Palomo-Irigoyen, Ashwin Woodhoo, Vega Villar-Suarez
Summary: The study investigated the therapeutic benefits of adipose tissue derived-mesenchymal stem cells (ASCs) and ASC-conditioned medium (CM) in promoting myelination and axonal regeneration. Results showed that both ASCs and CM could potentially aid in nerve regeneration, especially in reversing the effects of TNF-induced nerve damage on myelination-related genes and proteins.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Materials Science, Biomaterials
Xiangyun Yao, Zhiwen Yan, Xu Wang, Huiquan Jiang, Yun Qian, Cunyi Fan
Summary: Graphene and its derivatives have extraordinary electrochemical and mechanical properties, leading to extensive research in tissue engineering and regenerative medicine. Reduced graphene oxide (rGO) shares structural and functional similarities with graphene, influencing neurogenic differentiation of stem cells.
REGENERATIVE BIOMATERIALS
(2021)
Article
Engineering, Biomedical
Shaohua Wu, Ye Qi, Wen Shi, Mitchell Kuss, Shaojuan Chen, Bin Duan
Summary: This study developed conductive PPDO/CNT composite nanofiber yarns, demonstrating their potential for nerve regeneration applications. The PPDO/CNT NYs showed excellent mechanical properties and biocompatibility, and were able to promote cell differentiation and maturation through electrical stimulation.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Andrea Lavorato, Stefania Raimondo, Marina Boido, Luisa Muratori, Giorgia Durante, Fabio Cofano, Francesca Vincitorio, Salvatore Petrone, Paolo Titolo, Fulvio Tartara, Alessandro Vercelli, Diego Garbossa
Summary: The study discusses the features and applications of different types of mesenchymal stem cells in peripheral nerve regeneration after nerve injury. ADSCs are considered the most suitable MSCs for recovery from peripheral nerve lesions due to their excellent support and stimulation of axonal growth, remarkable paracrine activity, differentiation potential, low immunogenicity, and high survival rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Ana Carolina Correa de Assis, Amanda Luiza Silva Reis, Leonardo Vieira Nunes, Luiz Fernando Romanholo Ferreira, Muhammad Bilal, Hafiz M. N. Iqbal, Renato Nery Soriano
Summary: This review discusses the potential of using stem cells and tissue engineering to enhance peripheral nerve regeneration. Although the results are promising, there are still barriers to overcome such as immune response, oncogenic properties of stem cells, and the duration of regenerative effects.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Neurosciences
Xiangyun Yao, Zhiwen Yan, Xiaojing Li, Yanhao Li, Yuanming Ouyang, Cunyi Fan
Summary: Peripheral nerve injuries are common traumatic injuries worldwide, and the use of FK506-stimulated ADSCs has shown potential in promoting nerve regeneration, offering hope for the treatment of severe PNIs.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Omid Akhavan, Hossein Baharvand, Maryam Saadati, Hossein Fazli, Shiva Nemati
Summary: Ultrathin MoS2-MoO3-x heterojunction nanosheets were used as biocompatible and visible light-sensitive stimulator layers for controlled differentiation of human neural progenitor cells (hNPCs) into nervous lineages. The differentiation of hNPCs was increased on MoS2-MoO3-x due to biophysical cues and further increased on rGO/MoS2-MoO3-x due to a synergistic effect induced by the rGO. MoO3-x domains induced p-type doping in MoS2-MoO3-x, while photostimulation led to higher differentiation into glial cells. The presence of rGO in rGO/MoS2-MoO3-x promoted ultrafast electron transfer and increased neuronal differentiation of hNPCs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biotechnology & Applied Microbiology
Mohammad Soleimani, Kasra Cheraqpour, Raghuram Koganti, Seyed Mahbod Baharnoori, Ali R. Djalilian
Summary: The corneal epithelium consists of nonkeratinized stratified squamous cells, which undergo a significant turnover rate. The limbal epithelial stem cells (LESCs) located in the basal epithelial layer of the limbus play a crucial role in maintaining corneal homeostasis. The limbal stem cell niche (LSCN), composed of various cells, extracellular matrix, and signaling molecules, provides the microenvironment necessary for the proper functioning of LESCs. Damage to the LSCN can lead to limbal stem cell deficiency (LSCD) and conjunctivalization of the cornea. This review summarizes the basics of LSCN and discusses current and emerging bioengineering strategies for LSCN restoration to combat LSCD.
BIOENGINEERING-BASEL
(2023)
Article
Biology
M. Zanjirband, M. Baharlooie, Z. Safaeinejad, M. H. Nasr-Esfahani
Summary: This study investigated the novel therapeutics targets in PCOS by analyzing the transcriptome of granulosa cells using a drug repositioning approach. The results could provide valuable insights for the management of PCOS.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Polymer Science
Effat Nekoueiyfard, Fatemeh Radmanesh, Hossein Baharvand, Athar Mahdieh, Hamid Sadeghi-Abandansari, Rassoul Dinarvand
Summary: The main goal of many researchers is to introduce an intelligent pharmaceutical nanocarrier for targeted drug release and reducing the side effects of chemotherapy. In this study, a reduction-sensitive flower-like magnetomicelle containing doxorubicin (DOX) and magnetic iron oxide nanoparticles (Fe3O4) was synthesized by self-assembly of an amphiphilic triblock copolymer. The innovative design showed high drug loading content, reduced drug release in physiological conditions, fast degradation at the targeted site, and burst release of more drug content at an earlier time. Comparative studies demonstrated that the DOX-loaded magnetomicelle (D-LMM) exhibited superior cellular uptake and toxicity behavior on human breast adenocarcinoma cells compared to non-reduction sensitive control group, free DOX, and a commercial liposomal DOX product.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Medicine, Research & Experimental
Nasim Eslami, Khadijeh Bahrehbar, Fereshteh Esfandiari, Faezeh Shekari, Seyedeh-Nafiseh Hassani, Abdoreza Nazari, Mohammad Pakzad, Hossein Baharvand
Summary: Studies have shown that clonal mesenchymal stem cells (cMSCs) and their derived extracellular vesicles (MSC-EVs) can restore ovarian function in premature ovarian failure (POF). In this study, the therapeutic potential of a homogeneous population of cMSCs and their EV subpopulations was assessed in a mouse model of POF.
Article
Medicine, Research & Experimental
Mahsa Ghorbaninejad, Hamid Asadzadeh-Aghdaei, Hossein Baharvand, Anna Meyfour
Summary: Considering the limitations of 2D cell cultures and tissue models, creating intestinal organoids has become an ideal option for studying the complexity of the intestine. This review discusses the current advances in intestinal organoid development and their applications, particularly in disease modeling and epigenetics. The use of organoids in studying SARSCoV-2 infection and the potential of organoids in evaluating the effects of epigenetic drugs on gastrointestinal diseases are also explored.
Article
Multidisciplinary Sciences
Marjan Sadeghi, Mohsen Rahimi Andani, Mehdi Hajian, Nafiseh Sanei, Reza Moradi-Hajidavaloo, Nasrin Mahvash, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani
Summary: The role of the WNT/beta-catenin signaling pathway in preimplantation goat development was investigated. The expression of beta-CATENIN was observed in IVF and SCNT embryos, and inhibition of beta-catenin resulted in increased blastocyst formation rate. Different localization patterns of beta-catenin were observed in IVF and SCNT blastocysts. Inhibition of WNT signaling during the compact morula to blastocyst transition improved preimplantation embryonic development.
Article
Obstetrics & Gynecology
Aram Minas, Mazdak Razi, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Hashemi-Asl, Marziyeh Tavalaee
Summary: Varicocele (VCL) induces severe oxidative stress in testicular tissue, leading to infertility in 35% of males. This study compares the impact of varicose vessels on oxidative DNA damage and antioxidant reactions in the seminiferous tubules. The results show that varicocele causes significant DNA damage, reduces antioxidant reactions, and negatively affects the cell cycle progression.
REPRODUCTIVE SCIENCES
(2023)
Article
Neurosciences
Azadeh Amini, Faezeh Shekari, Sara Assar Kashani, Nasim Eslami, Abdoreza Nazari, Nahid Tofigh, Koorosh Shahpasand, Hamid Akbari Javar, Hossein Baharvand
Summary: This study investigated the therapeutic effects of clonal mesenchymal stem cell-derived extracellular vesicles (cMSC-EVs) in traumatic brain injury (TBI) treatment. The results showed that cMSC-EVs efficiently improved cognitive function in TBI animal models, reduced cis p-tau and cleaved caspase3 protein expression, and increased p-PI3K expression. Moreover, cMSC-EVs were able to effectively load an anti-cis p-tau antibody, suggesting their potential use in antibody delivery for passive immunotherapy.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Medicine, Research & Experimental
Sara Assar Kashani, Roghayeh Navabi, Azadeh Amini, Mostafa Hajinasrollah, Yaser Jenab, Shahram Rabbani, Abdoreza Nazari, Mohammad Pakzad, Maedeh Moazenchi, Mohammad Jafari Atrabi, Zakieh Samsonchi, Maryam Hezavehei, Elham Hosseini-Beheshti, Faezeh Shekari, Ensiyeh Hajizadeh-Saffar, Hossein Baharvand
Summary: This study investigated the therapeutic potential of a clonal population of mesenchymal stem cells (cMSC) and their extracellular vesicles (cMSC-EV) subpopulations in vitro and in a type 1 diabetes non-human primate model. The results showed that EV-S110K induced β-cell proliferation and reduced blood glucose levels, inflammatory cytokines, while increasing regulatory T cells and anti-inflammatory cytokines.
Article
Medicine, Research & Experimental
Somayeh Naderi, Zahra Shiri, Masoumeh Zarei-Kheirabadi, Sepideh Mollamohammadi, Parastoo Hosseini, Golnoosh Rahimi, Azadeh Moradmand, Azam Samadian, Amir Shojaei, Meghdad Yeganeh, Seyed Ahmad Mousavi, Motahare Badri, Adeleh Taei, Seyedeh-Nafiseh Hassani, Hossein Baharvand
Summary: This study aimed to generate transplantable midbrain dopaminergic (mDA) progenitors for treatment of Parkinson's Disease (PD). By optimizing the differentiation protocol using clinical-grade human embryonic stem cells (hESC), the researchers successfully obtained functional mDA progenitors. Transplantation of cryopreserved mDA progenitors into rats with 6-hydroxydopamine lesions resulted in functional recovery. This study provides a foundation for future translational applications.
Article
Biochemistry & Molecular Biology
Maryam Mirani, Soghra Bahmanpour, Fatemeh Masjedi, Zahra Derakhshan, Mahintaj Dara, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Bagher Tabei
Summary: This study reveals the negative effects of AGEs-RAGE axis on steroidogenesis in luteinized GCs, which can be mitigated by PM treatment. Understanding the interaction between AGEs and PM in ovarian physiology and pathology may lead to more targeted therapy for ovarian dysfunction.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Ali Moghimi-Khorasgani, Farshad Homayouni Moghadam, Mohammad Hossein Nasr-Esfahani
Summary: Microglial cells (MGCs) are resident macrophages in the brain and spinal cord, playing a crucial role in immune defense and transition between different states. Increased Nurr1 expression is associated with anti-neuroinflammatory responses. This study investigates the potential role of ferulic acid (FA) in facilitating microglia transition by inducing Nurr1 expression.
Article
Obstetrics & Gynecology
Saeideh Abdollahzadeh, Ahmad Riasi, Marziyeh Tavalaee, Farnoosh Jafarpour, M. H. Nasr-Esfahani
Summary: An imbalance between omega-6 and omega-3 fatty acids in sperm is correlated with lipid peroxidation and DNA damage in sperm, indicating a possible link to fertility potential. This study found that men with high DNA fragmentation had higher levels of saturated, monounsaturated, and omega-6 to omega-3 fatty acid ratios in sperm and seminal plasma compared to men with low DNA fragmentation. Additionally, there were positive associations between the omega-6 to omega-3 fatty acid ratio and sperm lipid peroxidation, DNA fragmentation, and residual histones in both sperm and seminal plasma.
REPRODUCTIVE SCIENCES
(2023)
Article
Multidisciplinary Sciences
M. Zanjirband, R. Hodayi, Z. Safaeinejad, M. H. Nasr-Esfahani, R. Ghaedi-Heydari
Summary: This study investigated the impact of the p53 pathway in enhancing apoptosis and abnormal function of granulosa cells. Results suggest that p53 target genes play a critical role in the pathogenesis of PCOS, specifically through the enhancement of apoptosis in granulosa cells.
SCIENTIFIC REPORTS
(2023)