Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 102, Issue 11, Pages 3965-3972Publisher
WILEY
DOI: 10.1002/jbm.a.35073
Keywords
microcapsule; RGD; scaffold; ligand density; microenvironment
Funding
- Ministerio de Ciencia e Innovacion, University of the Basque Country UPV/EHU [UFI11/32]
- FEDER [SAF2008-03157]
- Gobierno Vasco (Departamento de Educacion, Universidades e Investigacion)
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The inclusion of the tripeptide RGD (Arg-Gly-Asp) in otherwise inert biomaterials employed for cell encapsulation has been observed to be an effective strategy to provide the immobilized cells with a more suitable microenvironment. However, some controversial results collected during the last years, especially in vivo, have questioned its effectiveness. Here, we have studied the behavior of C2C12 myoblasts immobilized in alginate-poly-L-lysine-alginate microcapsules with different densities of RGD. The use of these microcapsules offer the advantage of avoiding native proteins influence permitting to establish direct comparisons between in vitro and in vivo assays. The results suggest that RGD-modified matrices provide higher dynamism, achieving therapeutically more active biosystems not only in vitro, but also in vivo. The highest functionality of the immobilized cells in vitro was obtained with the lowest RGD density. However, higher RGD densities were required in vivo to obtain the same effects observed in vitro. Altogether, these results suggest the lack of in vitro-in vivo correlation when cell behavior is evaluated within different RGD-tailored cell-loaded scaffolds. (C) 2014 Wiley Periodicals, Inc.
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