Article
Nanoscience & Nanotechnology
Igor da Silva Brum, Lucio Frigo, Paulo Goncalo Pinto dos Santos, Carlos Nelson Elias, Guilherme Aparecido Monteiro Duque da Fonseca, Jorge Jose de Carvalho
Summary: This study compared the tissue behavior of nano-hydroxyapatite/beta-tricalcium phosphate mixture (nano-HA/beta-TCP) and deproteinized bovine bone mineral (DBBM) in a rat calvarial defect model. The nano-HA/beta-TCP mixture showed enhanced bone formation and remodeling effects, making it suitable for guided bone regeneration and tissue regeneration in dental surgical procedures.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Manjushree M. Bahir, Archana Rajendran, Deepak Pattanayak, Nibedita Lenka
Summary: This study successfully fabricated 3D composite scaffolds mimicking the ECM of bone tissue using collagen type I and calcium phosphate nanomaterials. The scaffolds exhibited similar microstructure, surface area, porosity, and mechanical strength as trabecular bone, and influenced the bioactivity, biocompatibility, and osteogenic differentiation potential of gingival-derived mesenchymal stem cells. Among the three types of scaffolds, BCP/Col-I showed the highest surface area and porosity, and promoted the maximum osteogenic differentiation of gMSCs. Additionally, the release of low concentrations of Ca2+ ions from the scaffolds also facilitated the osteogenic differentiation of mesenchymal stem cells.
Article
Multidisciplinary Sciences
Joviniano Martins de Oliveira Junior, Pedro Giorgetti Montagner, Rafael Coutinho Carrijo, Elizabeth Ferreira Martinez
Summary: The study demonstrated the promising application of biphasic calcium phosphate bioceramics in repairing critical defects in rat calvaria, with no significant differences in inflammatory response and bone formation observed between different particle sizes of BCP.
SCIENTIFIC REPORTS
(2021)
Article
Orthopedics
Cheol-Hee Jeong, Jooseong Kim, Hyun Sil Kim, Song-Yi Lim, Dawool Han, Aaron J. Huser, Sang Bae Lee, Yeonji Gim, Jeong Hyun Ji, Dohun Kim, Amaal M. Aldosari, Kyelim Yun, Yoon Hae Kwak
Summary: In this study, three types of OCPs were compared to commercially available 8-TCP for their potential to promote bone formation in rat tibias with defects. The results showed that OCP/Alg was equivalent to 8-TCP in promoting bone bridge formation and had better biodegradability. OCP/Col accelerated bone formation in defect repair.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Biotechnology & Applied Microbiology
Elizaveta Kon, Francesca Salamanna, Giuseppe Filardo, Berardo Di Matteo, Nogah Shabshin, Jonathan Shani, Milena Fini, Francesco Perdisa, Annapaola Parrilli, Simone Sprio, Andrea Ruffini, Maurilio Marcacci, Anna Tampieri
Summary: The study investigated the use of biomorphic calcium phosphate bone scaffolds for the regeneration of load-bearing segmental bone defects, showing that the scaffolds promoted new bone formation and quality, with GB-1 scaffold exhibiting higher fracture strength and stability. The scaffolds also demonstrated increased levels of osteoid surface, osteoblast surface, and vessels/microvessels numbers compared to allograft, highlighting their potential for orthopedic applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Diana Griesiute, Eva Raudonyte-Svirbutaviciene, Aivaras Kareiva, Aleksej Zarkov
Summary: The present study investigates the influence of annealing conditions on phase transformations in calcium phosphates. The phase composition and Ca/P ratio in the final product highly depend on the annealing conditions due to the evaporation of phosphate species. Drastic changes in stoichiometry and phase composition were observed after annealing in a vacuum.
Article
Materials Science, Ceramics
Marzieh Taherimehr, Reza Bagheri, Masoumeh Taherimehr
Summary: The mechanical properties of TPS were enhanced by incorporating bioactive 8-TCP particles for bone tissue engineering applications. Analysis of the nanocomposites showed improved mechanical properties and bioactivity, making them suitable for use in bone tissue engineering.
CERAMICS INTERNATIONAL
(2021)
Article
Chemistry, Physical
Yun He, Yu Peng, Lishuang Liu, Sha Hou, Junyu Mu, Liang Lan, Lijia Cheng, Zheng Shi
Summary: This study compares the bone induction of five types of calcium phosphate biomaterials implanted in mice and explores the mechanisms of vascularization and particle-size-related osteoinduction. The results show that nanoscale Ca-P biomaterials can induce osteogenesis in mice muscle, with TCP showing higher osteoinductive effects compared to HA. The particle size of the biomaterials affects angiogenesis and osteogenesis, and there is a positive correlation between the number of blood vessels and the area percentage of new bone tissue.
Article
Chemistry, Physical
Jeong-Kui Ku, Il-hyung Kim, Jung Hee Shim, Yu Ha Kim, Baek Hyun Kim, Young-Kyun Kim, Pil-Young Yun
Summary: This study compares the biomechanical properties and bone regeneration of whitlockite with hydroxyapatite or beta-tricalcium phosphate-based materials. The results show that whitlockite has excellent volumetric stability and osteoconductivity, and it is similar to the other materials in terms of new bone formation.
Article
Biotechnology & Applied Microbiology
Xu Chen, Chun-Yan Gao, Xiao-Yang Chu, Chun-Yan Zheng, Ying-Yi Luan, Xin He, Kai Yang, Dong-Liang Zhang
Summary: This study synthesized a HG-HA-TCP scaffold loaded with VEGF, which showed good biocompatibility and mechanical properties. The results demonstrated that the scaffold could promote the proliferation, migration, and adhesion of stem cells, as well as enhance osteogenic differentiation and vascularization. In vivo experiments showed that the scaffold improved new bone regeneration and increased bone mineral density. This study is of great importance for bone tissue engineering and developing new clinical treatment strategies for bone tissue defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Su Yeon Kwon, Jung Hee Shim, Yu Ha Kim, Chang Su Lim, Seong Bae An, Inbo Han
Summary: Whitlockite plays a significant role in bone remodeling and formation in a mouse spinal fusion model, showing a higher new bone formation and better bone conductivity compared to hydroxyapatite (HAp) and beta-tricalcium phosphate (β-TCP).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Soshi Hamada, Yu Mori, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Kazuyoshi Baba, Itsuki Oizumi, Ryuichi Kanabuchi, Naohisa Miyatake, Toshimi Aizawa, Osamu Suzuki
Summary: This study evaluated the effectiveness of OCP/Gel composite material in treating severe bone defects using a rat model. The results showed that compared to Gel, the use of OCP/Gel induced faster bone remodeling and cortical bone repair, and performed better in terms of new bone formation and osteoblast activity. Therefore, OCP/Gel could be used as a bone replacement material for the treatment of severe bone defects.
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
(2022)
Article
Materials Science, Ceramics
Evgeniya A. Syukkalova, Anastasia V. Sadetskaya, Natalya D. Demidova, Natalia P. Bobrysheva, Mikhail G. Osmolowsky, Mikhail A. Voznesenskiy, Olga M. Osmolovskaya
Summary: In this study, calcium phosphate nanoparticles were synthesized using a scalable hydrothermal method with significantly lower holding time. The effects of surfactants and dopants on the nanoparticles formation process were discussed, with the possibility of regulating morphological parameters demonstrated. Additionally, the thermal properties of the nanoparticles were reported, showing the influence of surfactant presence on preventing sintering at high temperatures and shifting decomposition temperature to higher values.
CERAMICS INTERNATIONAL
(2021)
Article
Materials Science, Ceramics
Lauryna Sinusaite, Anton Popov, Eva Raudonyte-Svirbutaviciene, Jen-Chang Yang, Aivaras Kareiva, Aleksej Zarkov
Summary: The present study demonstrated that Mn doping significantly retards the hydrolysis rate of β-TCP. β-TCP doped with 0.5 and 1 mol% of Mn required 20 and 35 hours, respectively, for complete hydrolysis, compared to pristine β-TCP which fully hydrolyzed in 10 hours.
CERAMICS INTERNATIONAL
(2021)
Article
Chemistry, Multidisciplinary
Youssef El Moussaoui, Helene Terrisse, Sophie Quillard, Marie-Helene Ropers, Bernard Humbert
Summary: By characterizing the physicochemical properties of three different samples, it was found that the commercial tricalcium phosphate (TCP) food additive is actually hydroxyapatite (HA), which exists in nanometric dimensions with various particle shapes. In aqueous media, HA particles rapidly agglomerate or aggregate above pH 6 and are slowly dissolved in acidic environments (pH < 5) until complete dissolution at pH 2. Therefore, the classification of TCP as a nanomaterial on the European market raises concerns about its potential persistency in the gastrointestinal tract.
Review
Engineering, Biomedical
Aysegul Dede Eren, Steven Vermeulen, Tara C. Schmitz, Jasper Foolen, Jan de Boer
Summary: Cells and their surrounding extracellular matrix (ECM) participate in dynamic reciprocity to maintain tissue homeostasis. This involves the deposition of ECM by cells, which then presents the signals that define cell identity. The relationship between cell shape, ECM, and tissue homeostasis is important for understanding pathological conditions and for manipulating cell function in micro-fabricated platforms. This manuscript discusses the loop of phenotype in tendon tissue homeostasis, focusing on cell shape, ECM organization, and signal transduction pathways.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Steven Vermeulen, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. K. van der Veer, Dieter Deforce, Maarten Dhaenens, Jan de Boer
Summary: This study investigates how surface topography influences nuclear morphology, histone modifications, and gene expression. The authors find that micro-topographies result in a loss of histone acetylation and nucleoli abundance, as well as a reduction in gene expression associated with chromosome organization. They also show that micro-topographies can influence the proliferation and phenotype of mesenchymal stem cells, and this effect is reversible.
Article
Cell & Tissue Engineering
Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: The gut microbiome is crucial for proper gut function, but imbalances can lead to gastrointestinal conditions. Current models do not accurately depict in vivo situations, thus more advanced in vitro models are needed to study host-microbiome interactions. Here, we developed a novel hypoxia-tolerant apical-out small intestinal organoid model that mimics intestinal cell composition and structure. Co-cultures with anaerobic strains showed successful colonization and probiotic benefits. These organoids will help unravel unknown mechanisms and aid in the development of microbiome-related therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Panagiota Kakni, Carmen Lopez-Iglesias, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: Pluripotent stem cell-derived intestinal organoids with reversed epithelial polarity (apical-out) successfully recapitulate tissue-specific functions and show strong epithelial barrier formation, nutrient transport, active lipid metabolism, and expression of drug-metabolizing enzymes and transporters. This breakthrough enables high-throughput/high-content in vitro applications in the fields of nutrition, metabolism, and drug discovery.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Engineering, Biomedical
V. P. Galvan-Chacon, D. de Melo Pereira, S. Vermeulen, H. Yuan, J. Li, P. Habibovic
Summary: This study aimed to understand the effects of the chemical composition and surface microstructure of a beta-tricalcium phosphate (TCP) ceramic on the differentiation of human mesenchymal stromal cells (hMSCs). The results showed that the original ceramic was most potent in supporting osteogenic differentiation, but some effects of surface structure in the absence of inorganics were also observed.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Martyna Nikody, Jiaping Li, Elizabeth Rosado Balmayor, Lorenzo Moroni, Pamela Habibovic
Summary: This article introduces a polymer-ceramic composite material that enhances its osteogenic properties by adding zinc, for the treatment of critical-sized bone defects that cannot heal spontaneously and require surgical intervention. The study found that the addition of zinc can promote the differentiation of osteogenic cells, enhance extracellular matrix production and mineralization, and demonstrate the potential for treating critical-sized bone defects.
BIOMATERIALS ADVANCES
(2023)
Article
Biotechnology & Applied Microbiology
Rio P. Juni, Jordy M. M. Kocken, Ricardo C. Abreu, Lara Ottaviani, Tim Davalan, Burcu Duygu, Ella M. Poels, Aliaksei Vasilevich, Jana C. Hegenbarth, Mahesh Appari, Nicole Bitsch, Serve Olieslagers, Dorien M. Schrijvers, Monika Stoll, Joerg Heineke, Jan de Boer, Leon J. de Windt, Paula A. da Costa
Summary: While impaired myocardial vascularization contributes to a mismatch between myocardial oxygen demand and supply, the mechanistic basis for disrupted tissue growth and angiogenesis in heart failure remains poorly understood. Silencing strategies have identified microRNAs as crucial regulators of angiogenesis, with miR-216a being a pro-angiogenic microRNA enriched in cardiac microvascular endothelial cells. miR-216a null mice display cardiac phenotypes related to impaired myocardial vascularization, autophagy, and inflammation, indicating that microRNA regulation of microvascularization impacts the cardiac response to stress.
Article
Engineering, Biomedical
Danielle Baptista, Zeinab Tahmasebi Birgani, Helene Widowski, Fiona Passanha, Vasili Stylianidis, Kevin Knoops, Eva Gubbins, Cinta Iriondo, Kari-Pekka Skarp, Robbert J. Rottier, Tim G. Wolfs, Clemens van Blitterswijk, Vanessa LaPointe, Pamela Habibovic, Niki L. Reynaert, Stefan Giselbrecht, Roman Truckenmueller
Summary: This study investigated the feasibility of culturing human bronchial organoids in a polymer film-based microwell array platform. The organoids were preformed through the culture of single cells and then transferred into the microwells for further cultivation. Various microscopy techniques were employed to characterize the organoids, and the ease of micromanipulation in the microwells was demonstrated by microinjection.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Biomaterials
Ralph J. S. van Mechelen, Phani Sudarsanam, Christian J. F. Bertens, Mehmet O. Tas, Marion J. J. Gijbels, Leonard Pinchuk, Jan de Boer, Henny J. M. Beckers
Summary: Placement of a glaucoma shunt with an endplate can effectively lower intraocular pressure (IOP), but fibrosis at the outflow site can affect surgical outcomes.
Article
Nanoscience & Nanotechnology
Xingzhen Zhang, Zeynep Karagoz, Sangita Swapnasrita, Pamela Habibovic, Aureilie Carlier, Sabine van Rijt
Summary: This study investigated the effect of RGD adhesion ligand global density, spacing, and clustering levels on stem cell adhesion and differentiation. It was found that highly clustered RGD-MSN-based films with increased RGD global density promoted cell adhesion and spreading. Moreover, a higher RGD ligand clustering level also promoted the expression of alkaline phosphatase in hMSCs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Biomaterials
Aygul Zengin, Filipa Castro Teixeira, Tony Feliciano, Pamela Habibovic, Carlos Domingues Mota, Matthew B. Baker, Sabine van Rijt
Summary: In this study, a new type of nanocomposite bioinks containing thiolated mesoporous silica nanoparticles (MSN) was developed. The MSNs could rapidly crosslink hydrogels upon exposure to UV light, and modification of the MSNs' surface resulted in enzyme-responsive biodegradable bioinks. Addition of specific peptides enhanced cell-matrix interactions. The nanocomposite bioinks could be printed using extrusion-based bioprinting, and showed tunable mechanical and degradation properties.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Francesca Giacomini, David Baiao Barata, Hoon Suk Rho, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Stefan Giselbrecht, Roman Truckenmuller, Pamela Habibovic
Summary: Tendons are highly organized tissues that require aligned collagen fibers for proper function. This study developed a microfluidic platform that allows controlled formation and alignment of collagen fibers, leading to changes in cell morphology and marker expression in tenocytes. This research has implications for tendon regeneration and the development of physiologically relevant in vitro models.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Pichaporn Sutthavas, Matthias Schumacher, Martyna Nikody, Vaijayanthi Ramesh, Jurij Jakobi, Elizabeth R. R. Balmayor, Pamela Habibovic, Christoph Rehbock, Stephan Barcikowski, Sabine van Rijt
Summary: Bioactive glass nanoparticles (nBGs) have been successfully doped with iron, strontium, and/or copper ions using pulsed laser techniques. The laser-doped nBGs maintained their morphology and showed biocompatibility. Furthermore, the presence of specific ions in the nBGs enhanced bone repair and vessel formation.
MATERIALS ADVANCES
(2023)
Article
Materials Science, Biomaterials
Lei He, Pamela Habibovic, Sabine van Rijt
Summary: Three types of mesoporous silica nanoparticles (MSNs) were developed and investigated for their ability to deliver selenium. The nanoparticles showed cytotoxicity towards cancer cells and lower toxicity towards healthy osteoblasts. Additionally, the nanoparticles induced reactive oxygen species generation and cell apoptosis. These findings highlight the potential of MSNs as selenium delivery carriers for osteosarcoma therapy.
BIOMATERIALS SCIENCE
(2023)
Article
Engineering, Biomedical
Steven Vermeulen, Kevin Knoops, Hans Duimel, Maryam Parvizifard, Denis van Beurden, Carmen Lopez-Iglesias, Stefan Giselbrecht, Roman Truckenmueller, Pamela Habibovic, Zeinab Tahmasebi Birgani
Summary: This study presents a platform for creating spheroids of human mesenchymal stromal cells and demonstrates their potential application in regenerative medicine. The mineralized spheroids promote osteogenic gene expression and can be used to study small molecule perturbations and extracellular-mediated calcification.
MATERIALS TODAY BIO
(2023)