Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 102, Issue 4, Pages 1085-1091Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34773
Keywords
nerve graft; decellularized tissue; nerve injury; nerve regeneration; peripheral nerve
Funding
- Hospital for Sick Children Foundation
- Hospital for Sick Children Foundation Student Scholarship Program
- Ontario Ministry of Research and Innovation
- CIHR Health Professional Student Research Award
- Comprehensive Research Experience for Medical Students program
- University of Toronto
- IAMGold, Inc.
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Processed nerve allografts are increasingly used as off the shelf nerve replacements for surgically bridging nerve gaps. Benchmarking the regenerative capacity of a commercially available human-derived nerve or xenograft in a rat nerve injury model would provide a convenient platform for future studies seeking to modify the processed nerve graft. Human and rat processed nerve grafts were used to bridge a 14 mm defect in a Sprague-Dawley rat sciatic nerve. Reversed autografts served as a positive control group. Twelve weeks following surgery, the distal nerve stumps were retrograde labeled and harvested for histology and histomorphometry. The cross-sectional areas of the human- and rat-derived processed nerve grafts were similar. Neuron counts and myelinated axon counts following use of the human-derived processed xenografts were decreased compared with those obtained from both the rat-derived processed nerve allografts and the autografts; the rat-derived processed nerve allografts were statistically equivalent to autografts. Measures of nerve fiber diameter and myelination revealed inferior axon regeneration maturity in both processed nerve grafts compared with autografts. Processed xenografts showed significantly reduced regeneration compared with autografts or processed allografts indicating that cross-species immunological reactions are important considerations in this rat model. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1085-1091, 2014.
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