Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 101, Issue 3, Pages 873-883Publisher
WILEY
DOI: 10.1002/jbm.a.34383
Keywords
protein adsorption; functionalizing hydroxyapatite; amino acid treatment; proteins-materials interactions
Funding
- Australian Research Council [DP0986230]
- Australian Research Council [DP0986230] Funding Source: Australian Research Council
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Charged functional groups present on the surface of biomaterials play an important role to regulate the affinity and attachment of macromolecules, including proteins, on the surface of biomaterials. In this study, the protein adsorptive capacity of hydroxyapatite (HA) was regulated by introducing different amino acids during the precipitation of HA. After incubation of HA samples in 5000 mu g/mL lysozyme solution at pH 7.4 for 24 h, unmodified HA adsorbed 0.886 mg/m2 of lysozyme while amino acid-functionalized HA (AA-HA) particles demonstrated higher adsorption capacity ranging from 1.090 to 1.680 mg/m2. Incorporation of amino acids with longer side chain lengths decreased the crystallinity and increased the negative value of the surface charge of HA particles. The specific surface areas were significantly increased in the presence of amino acids. Protein loading capacity onto AA-HA was further enhanced by regulating the pH of working solution whereby the protein adsorption rate increased with decreasing the pH, while reverse trend obtained in unmodified HA. The study demonstrated that the amount of adsorbed lysozyme onto AA-HA particles was correlated with the particles' surface charges. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 873-883, 2013.
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