Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 97A, Issue 3, Pages 243-250Publisher
WILEY
DOI: 10.1002/jbm.a.33050
Keywords
regenerative medicine; biomaterial; RPE cells; AMD; elastin-like recombinamers
Funding
- Castilla and Leon Regenerative Medicine and Cell Therapy Network Center
- CIBER-BBN
- AECI (Part of the Spanish Ministry of Foreign Affairs)
- Junta de Castilla-Leon
- National Plan of I+D+I
- Spanish Institute of Health Carlos III (ISCIII)-Subdireccion General de Evaluacion y Fomento de la Investigacion (MICNN) [PS09/00938]
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The aim of this study is to investigate the use of elastin-like recombinamers (ELRs) as a substrate that can maintain the growth, phenotype, and functional characteristics of retinal pigment epithelial (RPE) cells efficiently and as a suitable carrier for the transplantation of autologous RPE cells for treatment of age-related macular degeneration (AMD). ELR films containing a bioactive sequence, RGD (ELR-RGD), and one with no specific sequence (ELR-IK) as control, were obtained by solvent-casting onto glass and subsequent cross-linking. ARPE19 cells were seeded on sterilized ELR films as well as on the control surfaces. Cells were analysed after 4, 24, 72, and 120 h to study cell adhesion, proliferation, cell viability, morphology, and specificity by staining with Trypan blue, DAPI, Rhodamin-Phalloidin and RPE65, ZO-1 antibodies and observing under fluorescence as well as electron microscope. ARPE19 cells seeded on both ELR films and controls were 100% viable and maintained their morphology and set of characteristics at the different time points studied. Cell proliferation on ELR-RGD was significantly higher than that found on ELR-IK at all time points, although it was less than the growth rate on polystyrene. ARPE19 cells grow well on ELR-RGD maintaining their phenotype. These results should be extended to further studies with fresh human RPE cells and in vivo studies to determine whether this ELR-RGD matrix could be used as a Bruch's membrane prosthesis and carrier for transplantation of RPE cells in patients suffering with AMD. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 97A: 243-250, 2011.
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