Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 100A, Issue 1, Pages 134-140Publisher
WILEY
DOI: 10.1002/jbm.a.33250
Keywords
polyethylenimine; polyamidoamine; PEGylation; gene delivery; transfection
Funding
- Application Foundation and Advanced Technology [11JCZDJC20600]
- National Natural Science Foundation of China [30870397, 30970741]
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Low-molecular weight polyethylenimine (LMW PEI) shows the advantage of low-cytotoxicity, but has been inefficient in gene delivery as a consequence of the low-charge density. A number of previous studies employed the approach of crosslinking to solve this problem. In this study, a starburst LMW PEI gene vector has been developed. It has a polyamidoamine (PAMAM) core conjugated with a shell composed of LWM PEI and polyethylene glycol (PEG), that is PAMAM-PEI-PEG. Plasmid DNA (pEGFP-N1) and human cervix epithelial carcinoma (HeLa) cells were used in the study. The results showed that the starburst LMW PEI could effectively condense DNA at N/P above 5. The polyplexes had a size of about 500 nm and a nearly neutral surface because of the PEG shielding effect. This novel gene vector is able to maintain the low-cytotoxicity of LMW PEI, whereas its transfection efficiency was significantly improved. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 134-140, 2012.
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