Article
Medicine, Research & Experimental
Yan Wu, Dan Li, Mingzheng Li
Summary: Current studies have discovered that specific physicochemical properties of sintered calcium phosphate (CaP) ceramics can stimulate bone formation in non-osseous sites, known as osteoinductive biomaterials. The underlying mechanism of osteoinduction remains unclear, inhibiting the design of biomaterials with enhanced osteogenic potential. Recent evidence suggests that osteoclasts may play a critical role in osteoinduction by recruiting mesenchymal stem cells (MSCs) and enhancing their osteogenic differentiation. However, it is still unknown whether osteoclasts can initiate biomaterial-induced ectopic bone formation.
MEDICAL HYPOTHESES
(2023)
Article
Engineering, Biomedical
Loraine Kouba, Joel Burgin, Gordian Born, Giuseppe Perale, Dirk J. Schaefer, Arnaud Scherberich, Sebastien Pigeot, Ivan Martin
Summary: We developed a composite material consisting of an osteoconductive material and a tissue engineered human cartilage extracellular matrix (ECM) with strong osteoinductive properties. The low amount of osteoinductive ECM enhanced host cell recruitment and promoted the formation of large vascularized bone structures in two different animal models, including a challenging prefabricated bone-flap model. This study demonstrates the potential of this off-the-shelf human material for accelerated healing in clinical applications.
ACTA BIOMATERIALIA
(2022)
Review
Cell Biology
Kourosh Ahmadzadeh, Margot Vanoppen, Carlos D. Rose, Patrick Matthys, Carine Helena Wouters
Summary: Monocytes and macrophages can fuse to form multinucleated giant cells, with different types and functions. Osteoclasts participate in bone remodeling under normal conditions but contribute to bone destruction and loss under pathological conditions. Foreign body giant cells are involved in immune reactions against foreign material, while Langhans giant cells are associated with granulomas in infectious and non-infectious diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Zhangling Nie, Zhiqiao Hu, Xiaodong Guo, Yu Xiao, Xian Liu, Joost D. de Bruijn, Chongyun Bao, Huipin Yuan
Summary: Innate immune responses, specifically M2 macrophages and osteoclastogenesis, play important roles in material-induced bone formation. The study found that M0 macrophages fused to osteoclasts when treated with M-CSF, RANKL, and IL-4, and this osteoclast formation was enhanced with IL-4 treatment. M2 macrophages and osteoclasts produced osteogenic factors that induced osteogenic differentiation of osteogenic precursor cells and led to bone formation.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Biomaterials
Ketki Holkar, Vaijayanti Kale, Ganesh Ingavle
Summary: One of the objectives of bone tissue engineering is to produce scaffolds that mimic the structure and function of natural bone extracellular matrix, and attract and differentiate mesenchymal stem cells into osteoblasts. This study investigated the use of mineralized microenvironments in hydrogel platforms to guide stem cell fate. Results showed that both direct encapsulation and sustained release of nano-hydroxyapatite (nHAp) enhanced osteogenesis, but direct loading of nHAp into the hydrogel increased its mechanical strength and swelling ratio. Biochemical and molecular studies also demonstrated improved osteoinductive and osteoconductive potential. This cost-effective and simple approach has potential applications in clinical settings.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Immunology
Caroline S. Simon, Vanessa S. Sturmer, Julien Guizetti
Summary: Regulating the number of progeny generated by replicative cell cycles is crucial for organisms to adapt to their environment. Some species, such as malaria-causing parasites, go through multinucleated cell stages, impacting the determination of progeny numbers. Parasites undergo four phases of extensive proliferation during their life cycle, producing varying numbers of progeny.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Jing Hong, Chizu Sanjoba, Wataru Fujii, Junya Yamagishi, Yasuyuki Goto
Summary: Leishmaniasis is a disease caused by infection with Leishmania parasites, which can lead to multinucleation of macrophages. Infection with Leishmania donovani induces multinucleation of bone marrow-derived macrophages (BMDMs), accompanied by increased expression of ATP6V0D2 protein. These infection-induced multinucleated macrophages exhibit enhanced phagocytic activity on red blood cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Ayumi Niwa, Hiroyuki Tomita, Naoki Watanabe, Shunya Kiriyama, Akira Hara, Takuji Tanaka
Summary: Gallbladder carcinosarcoma with osteoclast-like multinucleated giant cells is a rare form of gallbladder cancer, and its pathogenesis is largely unknown. This article presents a case of gallbladder carcinosarcoma with osteoclast-like multinucleated giant cells, in which the interaction of RANK-RANKL signaling was observed.
PATHOLOGY & ONCOLOGY RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Wenzheng Pan, Lin Zheng, Jiawei Gao, Lin Ye, Zhenzhong Chen, Shijie Liu, Bin Pan, Jiawei Fang, Hehuan Lai, Yejin Zhang, Kainai Ni, Chao Lou, Dengwei He
Summary: SIS3 inhibits osteoclastogenesis by suppressing Smad3 phosphorylation, reducing ROS production, and preventing osteoclast differentiation and maturation, making it a promising alternative therapy for osteoporosis.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Developmental Biology
Jorel R. Padilla, Lillie M. Ferreira, Eric S. Folker
Summary: Nuclear movement is crucial for cell development and function, and it is highly conserved. Multinucleated cells and syncytia play important roles in development and homeostasis.
Article
Biochemistry & Molecular Biology
Xochitl Morales-de la Cruz, Alejandra Mandujano-Chavez, Daniel R. Browne, Timothy P. Devarenne, Lino Sanchez-Segura, Mercedes G. Lopez, Edmundo Lozoya-Gloria
Summary: Botryococcus braunii produces liquid hydrocarbons that can be processed into combustion engine fuels, but its slow cell growth rate and differences in cell cycle and division processes exist between different races of the algae.
Article
Oncology
Guoju Hong, Zhenqiu Chen, Xiaorui Han, Lin Zhou, Fengxiang Pang, Rishana Wu, Yingshan Shen, Xiaoming He, Zhinan Hong, Ziqi Li, Wei He, Qiushi Wei
Summary: The study revealed that Robinin could prevent bone resorption by inhibiting osteoclastogenesis mediated by RANKL, reducing ROS production, and regulating molecular signaling pathways, making it a potential drug for treating osteoporosis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Microbiology
Christophe J. Queval, Antony Fearns, Laure Botella, Alicia Smyth, Laura Schnettger, Morgane Mitermite, Esen Wooff, Bernardo Villarreal-Ramos, Waldo Garcia-Jimenez, Tiaan Heunis, Matthias Trost, Dirk Werling, Francisco J. Salguero, Stephen Gordon, Maximiliano G. Gutierrez
Summary: The study revealed that Mbv and Mtb exhibit different behaviors in host cells, with Mbv infection leading to multinucleated cell formation in bovine macrophages. It was identified that MPB70 from Mbv and bovine macrophage extracellular vesicles are mechanistic drivers of multinucleated cell formation. In vivo confirmation of differential multinucleated cell formation in cattle models further supports the role of these factors in host tropism in human/bovine tuberculosis.
Article
Pathology
Gang Zheng, Rong He, Kaaren K. Reichard, Jess F. Peterson, Horatiu Olteanu, Jennifer L. Oliveira, Aruna Rangan, Dong Chen, Min Shi
Summary: In this study, 18 cases of increased multinucleated megakaryocytes in bone marrow biopsies were reported, suggesting that this phenomenon may not necessarily be associated with myelodysplastic syndrome. Follow-up of the patients post-surgery showed no new-onset cytopenia or myeloid neoplasm.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Perry C. Caviness, Dongzheng Gai, Oxana P. Lazarenko, Michael L. Blackburn, Fenghuang Zhan, Jin-Ran Chen
Summary: Osteoclasts derived from hematopoietic stem cells play a crucial role in bone resorption. Identifying new molecules that can epigenetically regulate osteoclastogenesis is essential for the development of novel treatments for bone deterioration disorders like osteoporosis. The protein Ezh2 has been found to be associated with the epigenetic regulation of various cellular processes, but its role in bone cell development is still not fully understood.
Article
Orthopedics
Hiromichi Hirai, Yoshiharu Nakaya, Yoshitada Usami, Atsushi Nakano, Takashi Fujishiro, Sachio Hayama, Masahiro Mizutani, Masashi Neo
JOURNAL OF ORTHOPAEDIC SCIENCE
(2023)
Article
Orthopedics
Gen Nakamura, Toshito Yasuda, Hiroaki Shima, Kosho Togei, Yoshihiro Hirai, Masashi Neo
Summary: The study aimed to determine the normal length and shape of the Achilles tendon (AT) and investigate correlating factors. Measurements were taken from 100 asymptomatic patients using magnetic resonance imaging. The results showed that 73% of the patients had an AT length within the range of 30-55 mm, and there was no correlation between tendon length and patient height. The study also found a strong correlation between AT length and the area of Kager's fat pad.
JOURNAL OF ORTHOPAEDIC SCIENCE
(2023)
Article
Orthopedics
Akihiko Hasegawa, Teruhisa Mihata, Kunimoto Fukunishi, Yasuo Itami, Akihiro Uchida, Masashi Neo
Summary: This study retrospectively investigated the graft tear rate, timing of graft tear, and the impact of graft tears on clinical outcomes after arthroscopic superior capsule reconstruction (SCR). The overall graft tear rate was 11.7%, with the majority of graft tears occurring within 6 months after surgery. Patients with graft tears had significantly inferior postoperative clinical scores compared to those without graft tears.
JOURNAL OF SHOULDER AND ELBOW SURGERY
(2023)
Article
Orthopedics
Yoshinori Okamoto, Hitoshi Wakama, Junya Matsuyama, Kaito Nakamura, Shuhei Otsuki, Masashi Neo
Summary: This study evaluated the association between the sagittal alignment of the pelvis and residual knee flexion contracture after total knee arthroplasty (TKA). The study found that a pelvic incidence >= 55 degrees and the femoral component flexion angle (FFA) were predictive factors for a residual flexion contracture >= 10 degrees.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Pharmacology & Pharmacy
Tomoyuki Yamada, Ryuji Kato, Yoshio Ijiri, Masami Nishihara, Masashi Neo
Summary: This study evaluated the risk of hepatic disorders in patients taking concomitant acetaminophen and immune checkpoint inhibitors (ICI) using a disproportionality analysis. The results showed that the risk of hepatic disorders was higher in patients taking concomitant acetaminophen and ICI compared to those only taking acetaminophen.
INTERNATIONAL JOURNAL OF CLINICAL PHARMACY
(2023)
Article
Pharmacology & Pharmacy
Ryosuke Ota, Takeo Hata, Atsushi Hirata, Takeshi Hamada, Masami Nishihara, Masashi Neo, Takahiro Katsumata
Summary: This retrospective cohort study examined the interaction between methotrexate and glucocorticoids in patients with rheumatoid arthritis using bDMARDs. The results showed that the interaction between the doses of methotrexate and glucocorticoids significantly influenced the risk of bacterial infections. Higher doses of glucocorticoids were a significant risk factor for bacterial infections when high doses of methotrexate were used.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Letter
Clinical Neurology
Kaito Nakamura, Yoshinori Okamoto, Hitoshi Wakama, Junya Matsuyama, Takashi Ishitani, Shuhei Otsuki, Masashi Neo
EUROPEAN SPINE JOURNAL
(2023)
Article
Clinical Neurology
Kaito Nakamura, Yoshinori Okamoto, Hitoshi Wakama, Junya Matsuyama, Takashi Ishitani, Shuhei Otsuki, Masashi Neo
Summary: This retrospective case-control study found an association between global spinal malalignment and rapid progression of hip arthrosis. Significant differences were identified in pelvic tilt, sagittal vertical axis, and T1 pelvic angle between the rapid progression group and the non-rapid progression group. Logistic regression analysis and ROC curve analysis showed that pelvic tilt, sagittal vertical axis, and T1 pelvic angle were predictive of rapid progression of hip arthrosis.
EUROPEAN SPINE JOURNAL
(2023)
Article
Orthopedics
Yoshihiro Hirai, Hiroaki Shima, Kosho Togei, Toshito Yasuda, Masashi Neo
Summary: Hallux valgus surgery and open reduction of the lesser metatarsophalangeal joints with metatarsal shortening osteotomy can help treat severe hallux valgus with lesser metatarsophalangeal joint dislocation effectively.
JOURNAL OF FOOT & ANKLE SURGERY
(2023)
Article
Clinical Neurology
Yoshinori Okamoto, Hitoshi Wakama, Junya Matsuyama, Kaito Nakamura, Takafumi Saika, Shuhei Otsuki, Masashi Neo
Summary: This study evaluated the association between preoperative global alignment and proportion score and low back pain after total hip arthroplasty. The results showed that patients with a relative pelvic version below -7 degrees and a relative lumbar lordosis below -15 degrees were more likely to experience low back pain after THA. The measurement of relative pelvic version may be a useful predictor of THA outcome.
EUROPEAN SPINE JOURNAL
(2023)
Article
Nutrition & Dietetics
Yoko Urashima, Tatsuya Ueno, Chiyuki Takeda, Hiroshi Kusaba, Rina Tanaka, Karin Noda, Kanako Kawakami, Takuo Murakami, Aoi Kawaguchi, Yuka Suemitsu, Kazuya Urashima, Kaoru Suzuki, Kazumi Kurachi, Masami Nishihara, Masashi Neo, Michiaki Myotoku, Takuro Kobori, Tokio Obata
Summary: Casein, digested soy protein, and dextrin decrease the gastric absorption of phenytoin, while digested casein further reduces the absorption by weakening tight junctions. On the other hand, digested casein and dextrin increase the permeability rate of phenytoin. These findings provide guidance for selecting enteral nutrients for orally administered phenytoin.
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
(2023)
Letter
Clinical Neurology
Takashi Fujishiro, Takuya Obo, Masahiro Mizutani, Yoshiharu Nakaya, Sachio Hayama, Yoshitada Usami, Masashi Neo
WORLD NEUROSURGERY
(2023)
Article
Orthopedics
Shuhei Otsuki, Shunsuke Sezaki, Yoshinori Okamoto, Takashi Ishitani, Hitoshi Wakama, Masashi Neo
Summary: This study evaluated the safety and efficacy of a novel meniscal scaffold for the treatment of irreparable meniscal injuries. The results showed that the scaffold could tolerate shear forces and had therapeutic potentials for irreparable meniscal tears in humans.
Article
Orthopedics
Yasuo Itami, Orr Limpisvasti, Michelle H. McGarry, Nilay A. Patel, Charles C. Lin, Thomas Dooney, Teruhisa Mihata, Masashi Neo, Thay Q. Lee
Summary: The purpose of this study was to compare the biomechanical characteristics of a new locking loop stitch (LLS) to the traditional Krackow stitch. The LLS showed less deformation, higher stiffness, and higher yield load compared to the Krackow stitch. Therefore, LLS can be a viable alternative to the Krackow stitch for graft fixation.
CLINICS IN ORTHOPEDIC SURGERY
(2023)
Article
Chemistry, Medicinal
T. Amadutsumi, Y. Urashima, K. Urashima, K. Suzuki, K. Kurachi, M. Nishihara, M. Neo, M. Myotoku, T. Kobori, T. Obata
Summary: This study investigated the interaction between levetiracetam (LEV) and enteral nutrients (ENs). Co-administration of LEV with Terumeal (R) Soft, an EN, reduced the absorption of LEV, which could be prevented by administering Terumeal (R) Soft 2 hours after LEV administration. Semi-solid Racol (R) NF altered the absorption of LEV, while liquid Racol (R) NF did not affect LEV pharmacokinetics.